The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
GENERATION OF CYTOTOXIC T LYMPHOCYTES IN VITRO
Open accessAbstract
Mouse cytotoxic T lymphocytes (CTL) were generated in mixed leukocyte cultures (MLC) using spleen cells as responding cells and irradiated allogeneic spleen cells as stimulating cells. Cytotoxicity was assessed by a quantitative 51Cr assay system and the relative frequency of CTL in individual cell populations was estimated from dose-response curves. Inclusion of 2-mercaptoethanol in the MLC medium resulted in a 20–40-fold increase in the relative number of CTL generated at the peak of the response. Under these culture conditions, cell-mediated cytotoxic activity was detectable in MLC populations as early as 48 h after the onset of the cultures. When spleen cells from mice immunized with allogeneic tumor cells 2–4 mo previously were cultured with irradiated spleen cells of the same alloantigenic specificity (MLC-Imm), it was found that the cell-mediated cytotoxic response was detectable earlier and reached higher levels than that observed in a primary MLC. At the peak of the response, MLC-Imm populations were observed to lyse up to 50% of the target cells within 3 h at a lymphocyte: target cell ratio of 0.3:1. Immunological and physical characterization of the effector cells generated in MLC-Imm indicated that they were medium to large-sized T lymphocytes. Altogether, these studies suggested the existence of an anamnestic cell-mediated cytotoxic response in MLC-Imm.
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Cites
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Self-citationCell-Mediated Cytotoxicity, Allograft Rejection, and Tumor Immunity
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Specific in vitro Cytotoxicity of Thymus-derived Lymphocytes sensitized to Alloantigens
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PROMOTION OF REPLICATION IN LYMPHOID CELLS BY SPECIFIC THIOLS AND DISULFIDES IN VITRO
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Enhancement of antibody synthesis in vitro by mercaptoethanol
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Cited by
Immunological memory and protective immunity: understanding their relation
Ahmed, R., & Gray, D. (1996) · Science · doi:10.1126/science.272.5258.54 · Cited by 1,775
Cytolytic T-cell cytotoxicity is mediated through perforin and Fas lytic pathways
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Mosley, B., Beckmann, M. P., March, C. J., Idzerda, R. L., Gimpel, S. D., VandenBos, T., Friend, D., Alpert, A., Anderson, D., Jackson, J., et al. (1989) · Cell · doi:10.1016/0092-8674(89)90295-x · Cited by 624
Idzerda, R. L., March, C. J., Mosley, B., Lyman, S. D., Vanden Bos, T., Gimpel, S. D., Din, W. S., Grabstein, K. H., Widmer, M. B., & Park, L. S. (1990) · The Journal of experimental medicine · doi:10.1084/jem.171.3.861 · Cited by 420
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Metadata
- Authors
- Cerottini, J.-C., Engers, H. D., MacDonald, H. R., & Brunner, K. T.
- Venue
- The Journal of Experimental Medicine
- Publisher
- Rockefeller University Press
- Published
- 1974-09-01
- Volume
- 140
- Issue
- 3
- Pages
- 703-717
- ISSN
- 0022-1007 (ISSN-L)
- 1540-9538
- OpenAlex
- W2142492759
- Cited by
- 478
- References
- 9
- FWCI
- 12.02
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Cerottini, J.-C., Engers, H. D., MacDonald, H. R., & Brunner, K. T. (1974). GENERATION OF CYTOTOXIC T LYMPHOCYTES IN VITRO. The Journal of Experimental Medicine. https://doi.org/10.1084/jem.140.3.703
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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