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The major histocompatibility complex-restricted antigen receptor on T cells. I. Isolation with a monoclonal antibody.

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Haskins, K, Kubo, R, White, J, Pigeon, M, Kappler, J, Marrack, P·1983·The Journal of experimental medicine

Abstract

An antibody-secreting B cell hybridoma, KJ1-26.1, has been prepared from mice immunized with the T cell hybridoma DO-11.10, which recognizes chicken ovalbumin in association with I-Ad (cOVA/I-Ad). KJ1-26.1 blocks I-restricted antigen recognition by DO-11.10 and a subclone of this T cell hybridoma, DO-11.10.24, which has the same specificity for cOVA/I-Ad as its parent. KJ1-26.1 does not block I-restricted antigen recognition by any other T cell hybridoma tested, including a number of T cell hybridomas closely related to DO-11.10, with similar, but not identical, specificities for antigen/I. Moreover, KJ1-26.1 binds to DO-11.10 and DO-11.10.24, but not to any other T cell hybridomas tested, including three subclones of DO-11.10 that have lost the ability to recognize cOVA/I-Ad. Thus, in every regard KJ1-26.1 appears to be binding to all or part of the receptors for antigen/I on the T cell hybridoma DO-11.10. KJ1-26.1 appears to bind to approximately 15,000 molecules/cell on the surface of DO-11.10. The antibody precipitates an 80,000 dimer from the cells, which on reduction migrates as 40-44,000 monomers. The receptor(s) for antigen/I on DO-11.10 therefore includes molecules with these properties.

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Metadata

Authors
Haskins, K., Kubo, R., White, J., Pigeon, M., Kappler, J., & Marrack, P.
Venue
The Journal of experimental medicine
Published
1983-04-01
Volume
157
Issue
4
Pages
1149-1169
ISSN
  • 0022-1007 (ISSN-L)
  • 1540-9538
OpenAlex
W2036160208
Cited by
846
References
1
FWCI
33.31
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: The major histocompatibility complex-restricted antigen receptor on T cells. I. Isolation with a monoclonal antibody. https://app.cochat.ai/papers/10.1084/jem.157.4.1149
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Haskins, K., Kubo, R., White, J., Pigeon, M., Kappler, J., & Marrack, P. (1983). The major histocompatibility complex-restricted antigen receptor on T cells. I. Isolation with a monoclonal antibody. The Journal of experimental medicine. https://doi.org/10.1084/jem.157.4.1149

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