The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
Endotoxin-responsive sequences control cachectin/tumor necrosis factor biosynthesis at the translational level.
Open accessAbstract
The biosynthesis of cachectin/TNF is largely regulated at a post-transcriptional level. Bacterial endotoxin, which strongly induces cachectin/TNF production, thus seems to elicit at least some of its effects by altering the macrophage cytoplasmic milieu. It has previously been shown that the 3'-untranslated TTATTTAT element present in numerous cytokines and proto-oncogenes is capable of repressing the translation of mRNA molecules in which it is represented. Using constructs in which the CAT coding sequence is followed by varying segments of the cachectin/TNF 3'-untranslated region, we now demonstrate that downstream sequences present in the cachectin/TNF mRNA are sufficient to mediate greater than 200-fold induction of CAT synthesis in response to activation by endotoxin. Induction of CAT activity is not attributable to a change in cytoplasmic mRNA concentration, but to a marked enhancement of translational efficiency. The response to endotoxin represents "derepression," and is conferred chiefly by the translationally repressive TTATTTAT element, acting in concert with essential flanking sequences.
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Cites
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Self-citationControl of Cachectin (Tumor Necrosis Factor) Synthesis: Mechanisms of Endotoxin Resistance
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Self-citationBasic methods in molecular biology
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Self-citationRemoval of poly(A) and consequent degradation of c-fos mRNA facilitated by 3′ AU-rich sequences
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Tili, E., Michaille, J.-J., Cimino, A., Costinean, S., Dumitru, C. D., Adair, B., Fabbri, M., Alder, H., Liu, C. G., Calin, G. A., et al. (2007) · The Journal of Immunology · doi:10.4049/jimmunol.179.8.5082 · Cited by 1,304
Kedersha, N. L., Gupta, M., Li, W., Miller, I., & Anderson, P. (1999) · The Journal of Cell Biology · doi:10.1083/jcb.147.7.1431 · Cited by 1,256
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Metadata
- Authors
- Han, J., Brown, T., & Beutler, B.
- Venue
- The Journal of experimental medicine
- Publisher
- Rockefeller University Press
- Published
- 1990-02-01
- Volume
- 171
- Issue
- 2
- Pages
- 465-475
- ISSN
- 0022-1007 (ISSN-L)
- 1540-9538
- OpenAlex
- W1893715971
- Cited by
- 477
- References
- 20
- FWCI
- 10.67
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Han, J., Brown, T., & Beutler, B. (1990). Endotoxin-responsive sequences control cachectin/tumor necrosis factor biosynthesis at the translational level. The Journal of experimental medicine. https://doi.org/10.1084/jem.171.2.465
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
