The DOI matches its Crossref record; 41 of the 41 references it cites were found in a registry or an index.
Interleukin 10(IL-10) inhibits cytokine synthesis by human monocytes: an autoregulatory role of IL-10 produced by monocytes.
Open accessAbstract
In the present study we demonstrate that human monocytes activated by lipopolysaccharides (LPS) were able to produce high levels of interleukin 10 (IL-10), previously designated cytokine synthesis inhibitory factor (CSIF), in a dose dependent fashion. IL-10 was detectable 7 h after activation of the monocytes and maximal levels of IL-10 production were observed after 24-48 h. These kinetics indicated that the production of IL-10 by human monocytes was relatively late as compared to the production of IL-1 alpha, IL-1 beta, IL-6, IL-8, tumor necrosis factor alpha (TNF alpha), and granulocyte colony-stimulating factor (G-CSF), which were all secreted at high levels 4-8 h after activation. The production of IL-10 by LPS activated monocytes was, similar to that of IL-1 alpha, IL-1 beta, IL-6, IL-8, TNF alpha, granulocyte-macrophage colony-stimulating factor (GM-CSF), and G-CSF, inhibited by IL-4. Furthermore we demonstrate here that IL-10, added to monocytes, activated by interferon gamma (IFN-gamma), LPS, or combinations of LPS and IFN-gamma at the onset of the cultures, strongly inhibited the production of IL-1 alpha, IL-1 beta, IL-6, IL-8, TNF alpha, GM-CSF, and G-CSF at the transcriptional level. Viral-IL-10, which has similar biological activities on human cells, also inhibited the production of TNF alpha and GM-CSF by monocytes following LPS activation. Activation of monocytes by LPS in the presence of neutralizing anti-IL-10 monoclonal antibodies resulted in the production of higher amounts of cytokines relative to LPS treatment alone, indicating that endogenously produced IL-10 inhibited the production of IL-1 alpha, IL-1 beta, IL-6, IL-8, TNF alpha, GM-CSF, and G-CSF. In addition, IL-10 had autoregulatory effects since it strongly inhibited IL-10 mRNA synthesis in LPS activated monocytes. Furthermore, endogenously produced IL-10 was found to be responsible for the reduction in class II major histocompatibility complex (MHC) expression following activation of monocytes with LPS. Taken together our results indicate that IL-10 has important regulatory effects on immunological and inflammatory responses because of its capacity to downregulate class II MHC expression and to inhibit the production of proinflammatory cytokines by monocytes.
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“IL-10 inhibits production of pro-inflammatory cytokines, such as TNF127.”
From Exerkines in health, resilience and disease (2022) · cites this paper as [127]
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Cites
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“Total ILNA was isolated from 20 x 106 monocytes by the guanidinium thioeyanateCsC1 procedure (22).”
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Self-citationIL-10 acts on the antigen-presenting cell to inhibit cytokine production by Th1 cells
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“-6 sense primer 5'-ATGAACTCCTTCTCCACAAGC-3' (nt 1 - 21), Ib6 anti sense primer 5'-CTACATTTGCCGAAGAGCCCTCAGGCTGGACTG-3' (nt 810 - 777) (17), Ib8 sense primer 5'-ATGACTTCCAAGCTGGCCGTG-3' (nt 1 - 21), I1-8 anti sense primer 5'-TTATGAATTCTCAGCCCTCTTCAAAAACTTCTC-Y (nt 302 - 269) (15), IL-10 sense primer 5'-ATGCCCCAAGCTGAGA…”
Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs
March, C. J., Mosley, B., Larsen, A., Cerretti, D. P., Braedt, G., Price, V. L., Gillis, S. A., Henney, C. S., Kronheim, S. R., Grabstein, K. H., et al. (1985) · Nature · doi:10.1038/315641a0 · Cited by 1,559 · cited 2×
“The following oligonucleotides were used for Southern analysis of PCR products: IL-lot: 5'-CATGGGTGCTTATAAGTCATC-Y (nt 500-521) (16); IL-1/3: 5'-CGATCACTGAACTGCACGCTCCGGG-3' (nt 444 469) (16); Ib6: 5'GAGGTATACCTAGAGTACCTC-Y (nt 510 - 531) (17); IL-8: 5'-TAAAGACATACTCCAAACCTT-3' (nt 200 - 221) (15); IL-10: 5'-CAGGTGAAG…”
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“Brain levels of TNF-α, IL-6, IL1β, and IL-10 were determined by enzyme immunoassay kits, as previously described (de Waal Malefyt et al., 1991) and according to the manufacturer's guidelines (eBioscience, Vienna, Austria).”
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Metadata
- Authors
- de Waal Malefyt, R., Abrams, J., Bennett, B., Figdor, C. G., & de Vries, J. E.
- Affiliations
- Department of Human Immunology, DNAX Research Institute, Palo Alto, California 94304.
- ORCID
- Figdor, C G
- Venue
- The Journal of experimental medicine
- Publisher
- Rockefeller University Press
- Published
- 1991-11-01
- Volume
- 174
- Issue
- 5
- Pages
- 1209-1220
- ISSN
- 0022-1007 (ISSN-L)
- 1540-9538
- PMC
- PMC2119001
- OpenAlex
- W2191654227
- Cited by
- 3,762
- References
- 41
- Citations by year
- 2026: 25 · 2025: 41 · 2024: 45 · 2023: 60 · 2022: 58 · 2021: 68 · 2020: 67 · 2019: 80 · 2018: 69 · 2017: 71 · 2016: 97 · 2015: 98 · 2014: 132 · 2013: 107 · 2012: 122
- FWCI
- 39.70
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by-nc-sa
- Locations
- The Journal of Experimental Medicine (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Radboud Repository (Radboud University) (submittedVersion)
- Radboud Repository (Radboud University) (submittedVersion, other-oa)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Cytokine Signaling Pathways and Interactions
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
- Keywords
- IL-10, monocytes, cytokine synthesis inhibition, lipopolysaccharide stimulation, interferon-gamma, autoregulation, cytokine gene expression, MHC class II expression, viral IL-10, TNF-α, GM-CSF, granulocyte colony-stimulating factor, interleukin-6, interleukin-8
- MeSH terms
- Histocompatibility Antigens Class II, Base Sequence, Cells, Cultured, Homeostasis, Humans, Interferon-gamma, Interleukin-1, Lipopolysaccharides, Molecular Sequence Data, Monocytes, RNA, Messenger, Transcription, Genetic, Interleukin-4, Interleukin-6, Granulocyte-Macrophage Colony-Stimulating Factor, Cytokines, Transforming Growth Factor beta, Interleukin-10
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