The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
Selective Suppression of Interleukin-12 Induction after Macrophage Receptor Ligation
Open accessAbstract
Interleukin (IL)-12 is a monocyte- and macrophage-derived cytokine that plays a crucial role in both the innate and the acquired immune response. In this study, we examined the effects that ligating specific macrophage receptors had on the induction of IL-12 by lipopolysaccharide (LPS). We report that ligation of the macrophage Fcγ, complement, or scavenger receptors inhibited the induction of IL-12 by LPS. Both mRNA synthesis and protein secretion were diminished to near-undetectable levels following receptor ligation. Suppression was specific to IL-12 since IL-10 and tumor necrosis factor-α (TNF-α) production were not inhibited by ligating macrophage receptors. The results of several different experimental approaches suggest that IL-12 downregulation was due to extracellular calcium influxes that resulted from receptor ligation. First, preventing extracellular calcium influxes, by performing the assays in EGTA, abrogated FcγR-mediated IL-12(p40) mRNA suppression. Second, exposure of macrophages to the calcium ionophores, ionomycin or A23187, mimicked receptor ligation and inhibited IL-12(p40) mRNA induction by LPS. Finally, bone marrow–derived macrophages from FcR γ chain–deficient mice, which fail to flux calcium after receptor ligation, failed to inhibit IL-12(p40) mRNA induction. These results indicate that the calcium influxes that occur as a result of receptor ligation are responsible for inhibiting the induction of IL-12 by LPS. Hence, the ligation of phagocytic receptors on macrophages can lead to a dramatic decrease in IL-12 induction. This downregulation may be a way of limiting proinflammatory responses of macrophages to extracellular pathogens, or suppressing the development of cell-mediated immunity to intracellular pathogens.
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Cites
Development of T H 1 CD4 + T Cells Through IL-12 Produced by Listeria -Induced Macrophages
Hsieh, C.-S., Macatonia, S. E., Tripp, C. S., Wolf, S. F., O'Garra, A., & Murphy, K. M. (1993) · Science · doi:10.1126/science.8097338 · Cited by 3,134
Kobayashi, M., Fitz, L., Ryan, M., Hewick, R. M., Clark, S. C., Chan, S., Loudon, R., Sherman, F., Perussia, B., & Trinchieri, G. (1989) · The Journal of experimental medicine · doi:10.1084/jem.170.3.827 · Cited by 1,953
Manetti, R., Parronchi, P., Giudizi, M. G., Piccinni, M. P., Maggi, E., Trinchieri, G., & Romagnani, S. (1993) · The Journal of experimental medicine · doi:10.1084/jem.177.4.1199 · Cited by 1,665
D'Andrea, A., Aste-Amezaga, M., Valiante, N. M., Ma, X., Kubin, M., & Trinchieri, G. (1993) · The Journal of experimental medicine · doi:10.1084/jem.178.3.1041 · Cited by 1,385
D'Andrea, A., Rengaraju, M., Valiante, N. M., Chehimi, J., Kubin, M., Aste, M., Chan, S. H., Kobayashi, M., Young, D., & Nickbarg, E. (1992) · The Journal of experimental medicine · doi:10.1084/jem.176.5.1387 · Cited by 1,144
IL-12-Deficient Mice Are Defective in IFNγ Production and Type 1 Cytokine Responses
Magram, J., Connaughton, S. E., Warrier, R. R., Carvajal, D. M., Wu, C.-Y., Ferrante, J., Stewart, C., Sarmiento, U., Faherty, D. A., & Gately, M. K. (1996) · Immunity · doi:10.1016/s1074-7613(00)80413-6 · Cited by 989
Seder, R. A., Gazzinelli, R., Sher, A., & Paul, W. E. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.21.10188 · Cited by 976
FcR γ chain deletion results in pleiotrophic effector cell defects
Takai, T., Li, M., Sylvestre, D., Clynes, R., & Ravetch, J. V. (1994) · Cell · doi:10.1016/0092-8674(94)90115-5 · Cited by 951
Self-citationWolf, S. F., Temple, P. A., Kobayashi, M., Young, D., Dicig, M., Lowe, L., Dzialo, R., Fitz, L., Ferenz, C., & Hewick, R. M. (1991) · The Journal of Immunology · doi:10.4049/jimmunol.146.9.3074 · Cited by 845
Tripp, C. S., Wolf, S. F., & Unanue, E. R. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.8.3725 · Cited by 776
Cited by
Protective and pathogenic functions of macrophage subsets
Murray, P. J., & Wynn, T. A. (2011) · Nature Reviews Immunology · doi:10.1038/nri3073 · Cited by 5,269
The many faces of macrophage activation
Mosser, D. M. (2003) · Journal of Leukocyte Biology · doi:10.1189/jlb.0602325 · Cited by 1,902
Self-citationCollaborative Induction of Inflammatory Responses by Dectin-1 and Toll-like Receptor 2
Gantner, B. N., Simmons, R. M., Canavera, S. J., Akira, S., & Underhill, D. M. (2003) · The Journal of Experimental Medicine · doi:10.1084/jem.20021787 · Cited by 1,643
Sutterwala, F. S., Ogura, Y., Szczepanik, M., Lara-Tejero, M., Lichtenberger, G. S., Grant, E. P., Bertin, J., Coyle, A. J., Galán, J. E., Askenase, P. W., et al. (2006) · Immunity · doi:10.1016/j.immuni.2006.02.004 · Cited by 980
Interleukin-12: A Cytokine at the Interface of Inflammation and Immunity
Trinchieri, G. (1998) · Advances in Immunology · doi:10.1016/s0065-2776(08)60387-9 · Cited by 760
Metadata
- Authors
- Sutterwala, F. S., Noel, G. J., Clynes, R., & Mosser, D. M.
- Venue
- The Journal of Experimental Medicine
- Publisher
- Rockefeller University Press
- Published
- 1997-06-02
- Volume
- 185
- Issue
- 11
- Pages
- 1977-1985
- ISSN
- 0022-1007 (ISSN-L)
- 1540-9538
- OpenAlex
- W2101647368
- Cited by
- 351
- References
- 39
- FWCI
- 7.64
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Sutterwala, F. S., Noel, G. J., Clynes, R., & Mosser, D. M. (1997). Selective Suppression of Interleukin-12 Induction after Macrophage Receptor Ligation. The Journal of Experimental Medicine. https://doi.org/10.1084/jem.185.11.1977
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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