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Cited by 129HIV Research and TreatmentTelomeres, Telomerase, and SenescenceHIV-related health complications and treatments

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Telomere Length, Telomerase Activity, and Replicative Potential in HIV Infection: Analysis of CD4+ and CD8+T Cells from HIV-discordant Monozygotic Twins

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Palmer, Larry D., Weng, Nan-ping, Levine, Bruce L., June, Carl H., Lane, H. Clifford, Hodes, Richard J.·1997·The Journal of Experimental Medicine
DOI 10.1084/jem.185.7.1381PMC PMC2196247
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Abstract

To address the possible role of replicative senescence in human immunodeficiency virus (HIV) infection, telomere length, telomerase activity, and in vitro replicative capacity were assessed in peripheral blood T cells from HIV+ and HIV− donors. Genetic and age-specific effects on these parameters were controlled by studying HIV-discordant pairs of monozygotic twins. Telomere terminal restriction fragment (TRF) lengths from CD4+ T cells of HIV+ donors were significantly greater than those from HIV− twins. In contrast, telomere lengths in CD8+ T cells from HIV+ donors were shorter than in HIV− donors. The in vitro replicative capacity of CD4+ cells from HIV+ donors was equivalent to that of HIV− donors in response to stimulation through T cell receptor CD3 and CD28. Little or no telomerase activity was detected in freshly isolated CD4+ or CD8+ lymphocytes from HIV+ or HIV− donors, but was induced by in vitro stimulation of both HIV+ and HIV− donor cells. These results suggest that HIV infection is associated with alterations in the population dynamics of both CD4+ and CD8+ T cells, but fail to provide evidence for clonal exhaustion or replicative senescence as a mechanism underlying the decline in CD4+ T cells of HIV-infected donors.

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Figure 1 Telomere length in CD4+ and CD8+ T cells. Genomic DNA was isolated from purified peripheral blood CD4+ and CD8+ T cells, digested, separated by electrophoresis, and the dried and denatured gel was hybridized with a 32P end-labeled oligonucleotide (CCCTAA)3 probe. Hybridized gels were subjected to PhosphorImager analysis.
Figure 1 Telomere length in CD4+ and CD8+ T cells. Genomic DNA was isolated from purified peripheral blood CD4+ and CD8+ T cells, digested, separated by electrophoresis, and the dried and denatured gel was hybridized with a 32P end-labeled oligonucleotide (CCCTAA)3 probe. Hybridized gels were subjected to PhosphorImager analysis.CC BY-NC-SA · PubMed Central
Figure 2 Telomere length in CD4+ and CD8+ T cells from HIV-positive and -negative twins. TRF analysis was carried out as described in Fig. 1, and mean TRF length calculated. (A) TRF length is shown for CD4+ T cells from HIV-negative (stippled bars) and HIV-positive (closed bars) twin donors. (B) TRF length is shown for CD8+ T cells from HIVnegative (stippled bars) and HIV-positive (closed bars) twin donors. (C) The difference in TRF length for HIV-positive and HIV-negative twin donors is shown for CD4+ (closed bar) and CD8+ (stippled bar) T cells. Positive values indicate TRF length that is greater in the HIV-positive twin, and negative values indicate TRF length that is greater in the HIV-negative twin.
Figure 2 Telomere length in CD4+ and CD8+ T cells from HIV-positive and -negative twins. TRF analysis was carried out as described in Fig. 1, and mean TRF length calculated. (A) TRF length is shown for CD4+ T cells from HIV-negative (stippled bars) and HIV-positive (closed bars) twin donors. (B) TRF length is shown for CD8+ T cells from HIVnegative (stippled bars) and HIV-positive (closed bars) twin donors. (C) The difference in TRF length for HIV-positive and HIV-negative twin donors is shown for CD4+ (closed bar) and CD8+ (stippled bar) T cells. Positive values indicate TRF length that is greater in the HIV-positive twin, and negative values indicate TRF length that is greater in the HIV-negative twin.CC BY-NC-SA · PubMed Central
Figure 3 Telomerase activity in T cells from HIV-positive and HIVnegative donors. CD4+ and CD8+ T cells were isolated from HIV-positive and HIV-negative donors by negative selection. Telomerase activity was assayed in freshly isolated cells and after in vitro stimulation with antiCD3 and anti-CD28. T cells were stimulated for 5 d with a combination of bead-bound anti-CD3 and CD28 mAbs. Results shown are representative of 15 HIV+ donors and their HIV− controls. 293 is a transformed human kidney cell line that expresses high levels of telomerase and is used here as a positive control. HUVEC are normal epithelial cells isolated from human umbilical vein used here as a negative control.
Figure 3 Telomerase activity in T cells from HIV-positive and HIVnegative donors. CD4+ and CD8+ T cells were isolated from HIV-positive and HIV-negative donors by negative selection. Telomerase activity was assayed in freshly isolated cells and after in vitro stimulation with antiCD3 and anti-CD28. T cells were stimulated for 5 d with a combination of bead-bound anti-CD3 and CD28 mAbs. Results shown are representative of 15 HIV+ donors and their HIV− controls. 293 is a transformed human kidney cell line that expresses high levels of telomerase and is used here as a positive control. HUVEC are normal epithelial cells isolated from human umbilical vein used here as a negative control.CC BY-NC-SA · PubMed Central
Figure 4 Replicative potential of CD4+ T cells from HIV-positive and HIV-negative donors. CD4+ T cells were repeatedly stimulated with immobilized anti-CD3 and anti-CD28. Population expansion was measured until cell cultures were unresponsive to further stimulation. The data shown are representative of five twin pairs studied.
Figure 4 Replicative potential of CD4+ T cells from HIV-positive and HIV-negative donors. CD4+ T cells were repeatedly stimulated with immobilized anti-CD3 and anti-CD28. Population expansion was measured until cell cultures were unresponsive to further stimulation. The data shown are representative of five twin pairs studied.CC BY-NC-SA · PubMed Central

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Metadata

Authors
Palmer, L. D., Weng, N.-P., Levine, B. L., June, C. H., Lane, H. C., & Hodes, R. J.
Affiliations
National Institutes of Health (US)
ORCID
Venue
The Journal of Experimental Medicine
Publisher
Rockefeller University Press
Published
1997-04-07
Volume
185
Issue
7
Pages
1381-1386
ISSN
  • 0022-1007 (ISSN-L)
  • 1540-9538
DOI
10.1084/jem.185.7.1381
PMC
PMC2196247
OpenAlex
W2114119969
Cited by
129
References
20
Citations by year
2026: 1 · 2024: 2 · 2022: 1 · 2021: 3 · 2019: 1 · 2018: 4 · 2017: 3 · 2016: 2 · 2015: 1 · 2014: 3 · 2013: 5 · 2012: 2
FWCI
5.96
Type
journal article
Language
en
Open access
Locations
  • The Journal of Experimental Medicine (publishedVersion)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Telomeres, Telomerase, and Senescence
  • Health Sciences › Medicine › Infectious Diseases › HIV-related health complications and treatments
Keywords
HIV infection, telomere length, telomerase activity, replicative senescence, CD4+ T cells, CD8+ T cells, terminal restriction fragments, CD4+ T cell decline
MeSH terms
Adult, Cells, Cultured, Humans, Lymphocyte Activation, Middle Aged, Twins, Monozygotic, CD4-Positive T-Lymphocytes, HIV Infections, T-Lymphocyte Subsets, Telomere, Cellular Senescence, CD8-Positive T-Lymphocytes, Telomerase

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