The DOI matches its Crossref record; 55 of the 55 references it cites were found in a registry or an index.
A Novel Sialic Acid Binding Site on Factor H Mediates Serum Resistance of Sialylated Neisseria gonorrhoeae
Open accessAbstract
Factor H (fH), a key alternative complement pathway regulator, is a cofactor for factor I–mediated cleavage of C3b. fH consists of 20 short consensus repeat (SCR) domains. Sialic acid binding domains have previously been localized to fH SCRs 6–10 and 13. To examine fH binding on a sialylated microbial surface, we grew Neisseria gonorrhoeae in the presence of 5′-cytidinemonophospho-N-acetylneuraminic acid, which sialylates lipooligosaccharide and converts to serum resistance gonococci previously sensitive to nonimmune serum killing. fH domains necessary for binding sialylated gonococci were determined by incubating organisms with recombinant human fH (rH) and nine mutant rH molecules (deletions spanning the entire fH molecule). rH and all mutant rH molecules that contained SCRs 16–20 bound to the sialylated strain; no mutant molecule bound to serum-sensitive nonsialylated organisms. Sialic acid was demonstrated to be the fH target by flow cytometry that showed a fourfold increase in fH binding that was reversed by neuraminidase-mediated cleavage of sialic acid off gonococci. Functional specificity of fH was confirmed by decreased total C3 binding and almost complete conversion to iC3b on sialylated gonococci. Sialic acid can therefore bind fH uniquely through SCRs 16–20. This blocks complement pathway activation for N. gonorrhoeae at the level of C3.
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Advances in the Biology and Chemistry of Sialic Acids
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Complement control protein factor H: The good, the bad, and the inadequate
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Metadata
- Authors
- Ram, S., Sharma, A. K., Simpson, S. D., Gulati, S., McQuillen, D. P., Pangburn, M. K., & Rice, P. A.
- Venue
- The Journal of Experimental Medicine
- Publisher
- Rockefeller University Press
- Published
- 1998-03-02
- Volume
- 187
- Issue
- 5
- Pages
- 743-752
- ISSN
- 0022-1007 (ISSN-L)
- 1540-9538
- OpenAlex
- W2073745575
- Cited by
- 398
- References
- 55
- FWCI
- 6.81
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: A Novel Sialic Acid Binding Site on Factor H Mediates Serum Resistance of Sialylated Neisseria gonorrhoeae. https://app.cochat.ai/papers/10.1084/jem.187.5.743
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- Ram, S., Sharma, A. K., Simpson, S. D., Gulati, S., McQuillen, D. P., Pangburn, M. K., & Rice, P. A. (1998). A Novel Sialic Acid Binding Site on Factor H Mediates Serum Resistance of Sialylated Neisseria gonorrhoeae. The Journal of Experimental Medicine. https://doi.org/10.1084/jem.187.5.743
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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