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Cited by 111Cellular transport and secretionMast cells and histamineCalcium signaling and nucleotide metabolism

Synaptotagmin II Negatively Regulates Ca2+-triggered Exocytosis of Lysosomes in Mast Cells

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Baram, Dana, Adachi, Roberto, Medalia, Ora, Tuvim, Michael, Dickey, Burton F., Mekori, Yoseph A., Sagi-Eisenberg, Ronit·1999·The Journal of Experimental Medicine
DOI 10.1084/jem.189.10.1649PMC PMC2193646
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Abstract

Synaptotagmins (Syts) I and II are believed to act as Ca2+ sensors in the control of neurotransmission. Here we demonstrate that mast cells express Syt II in their lysosomal fraction. We further show that activation of mast cells by either aggregation of FcεRI or by Ca2+ ionophores results in exocytosis of lysosomes, in addition to the well documented exocytosis of their secretory granules. Syt II directly regulates lysosomal exocytosis, whereby overexpression of Syt II inhibited Ca2+-triggered release of the lysosomal processed form of cathepsin D, whereas suppression of Syt II expression markedly potentiated this release. These findings provide evidence for a novel function of Syt II in negatively regulating Ca2+-triggered exocytosis of lysosomes, and suggest that Syt II–regulated secretion from lysosomes may play an important role in mast cell biology.

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  • Syts constitute a group of membrane proteins that regulate vesicle docking and fusion in processes such as exocytosis and phagocytosis.

    “Syts constitute a group of membrane proteins that regulate vesicle docking and fusion in processes such as exocytosis (11, 23) and phagocytosis (11, 24, 25).”

    From Macrophage Cytokines: Involvement in Immunity and Infectious Diseases (2014) · cites this paper as [23]

    The Discussion (page not available) identifies Syt II as a regulator of lysosomal exocytosis in mast cells and proposes that it may control a granule-fusion event. Our copy does not establish a role for synaptotagmins in phagocytosis or specifically in vesicle docking. (LLM, conf=0.86)

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Cited by

Sorted by citations

Authors
Baram, D., Adachi, R., Medalia, O., Tuvim, M., Dickey, B. F., Mekori, Y. A., & Sagi-Eisenberg, R.
Affiliations
  • Tel Aviv University (IL)
  • From the *Department of Cell Biology and Histology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, 69978, Israel; the ‡Allergy and Clinical Immunology Unit, Sapir Medical Center, Kfar Saba, 44281, Israel; and the §Departments of Medicine and Cell Biology, Baylor College of Medicine, Houston, Texas 77030
ORCID
Venue
The Journal of Experimental Medicine
Publisher
Rockefeller University Press
Published
1999-05-17
Volume
189
Issue
10
Pages
1649-1658
ISSN
  • 0022-1007 (ISSN-L)
  • 1540-9538
DOI
10.1084/jem.189.10.1649
PMC
PMC2193646
OpenAlex
W2042316173
Cited by
111
References
2
Citations by year
2025: 3 · 2024: 3 · 2022: 1 · 2021: 1 · 2019: 2 · 2018: 7 · 2017: 2 · 2016: 4 · 2015: 2 · 2014: 7 · 2013: 2 · 2012: 3
FWCI
3.43
Type
journal article
Language
en
Open access
Locations
  • The Journal of Experimental Medicine (publishedVersion)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
Funders
  • Israel Science Foundation
  • Tel Aviv University
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Immunology and Microbiology › Immunology › Mast cells and histamine
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Calcium signaling and nucleotide metabolism
Keywords
synaptotagmin II, mast cells, lysosomal exocytosis, calcium-triggered exocytosis, cathepsin D, calcium ionophore, lysosomes, secretory granules, calcium sensor
MeSH terms
Calcimycin, Animals, beta-N-Acetylhexosaminidases, Calcium, Cathepsin D, Cytoplasmic Granules, Exocytosis, Gene Expression Regulation, Lysosomes, Mast Cells, Mice, Inbred BALB C, Nerve Tissue Proteins, RNA, Messenger, Serotonin, Tetradecanoylphorbol Acetate, Transfection, Rats, Wistar, Receptors, IgE, Synaptotagmin II, Mice, Rats

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