The DOI matches its Crossref record; 60 of the 60 references it cites were found in a registry or an index.
Inhibition of Ca2+ Signaling by Mycobacterium tuberculosis Is Associated with Reduced Phagosome–Lysosome Fusion and Increased Survival within Human Macrophages
Open accessAbstract
Complement receptor (CR)-mediated phagocytosis of Mycobacterium tuberculosis by macrophages results in intracellular survival, suggesting that M. tuberculosis interferes with macrophage microbicidal mechanisms. As increases in cytosolic Ca2+ concentration ([Ca2+]c) promote phagocyte antimicrobial responses, we hypothesized that CR phagocytosis of M. tuberculosis is accompanied by altered Ca2+ signaling. Whereas the control complement (C)-opsonized particle zymosan (COZ) induced a 4.6-fold increase in [Ca2+]c in human macrophages, no change in [Ca2+]c occurred upon addition of live, C-opsonized virulent M. tuberculosis. Viability of M. tuberculosis and ingestion via CRs was required for infection of macrophages in the absence of increased [Ca2+]c, as killed M. tuberculosis or antibody (Ab)-opsonized, live M. tuberculosis induced elevations in [Ca2+]c similar to COZ. Increased [Ca2+]c induced by Ab-opsonized bacilli was associated with a 76% reduction in intracellular survival, compared with C-opsonized M. tuberculosis. Similarly, reversible elevation of macrophage [Ca2+]c with the ionophore A23187 reduced intracellular viability by 50%. Ionophore-mediated elevation of [Ca2+]c promoted the maturation of phagosomes containing live C-opsonized bacilli, as evidenced by acidification and accumulation of lysosomal protein markers. These data demonstrate that M. tuberculosis inhibits CR-mediated Ca2+ signaling and indicate that this alteration of macrophage activation contributes to inhibition of phagosome–lysosome fusion and promotion of intracellular mycobacterial survival.
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Cites
A new generation of Ca2+ indicators with greatly improved fluorescence properties.
Grynkiewicz, G., Poenie, M., & Tsien, R. Y. (1985) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)83641-4 · Cited by 21,607
Calcium's Role in Mechanotransduction during Muscle Development
Benavides Damm, T., & Egli, M. (2014) · Cellular Physiology and Biochemistry · doi:10.1159/000356667 · Cited by 14,502
Polazzi, E., Mengoni, I., Caprini, M., Peña-Altamira, E., Kurtys, E., & Monti, B. (2013) · Neurosignals · doi:10.1159/000337115 · Cited by 8,994
Ashburner, M. (1989) · Cold Spring Harbor Laboratory eBooks · Cited by 6,756
Thastrup, O., Cullen, P. J., Drøbak, B. K., Hanley, M. R., & Dawson, A. P. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.7.2466 · Cited by 3,270
Clapham, D. E. (1995) · Cell · doi:10.1016/0092-8674(95)90408-5 · Cited by 1,959
Sturgill-Koszycki, S., Schlesinger, P. H., Chakraborty, P., Haddix, P. L., Collins, H. L., Fok, A. K., Allen, R. D., Gluck, S. L., Heuser, J., & Russell, D. G. (1994) · Science · doi:10.1126/science.8303277 · Cited by 1,302
Structure and function of the leukocyte adhesion molecules CD11/CD18
Arnaout, M. (1990) · Blood · doi:10.1182/blood.v75.5.1037.1037 · Cited by 1,035
Elementary and global aspects of calcium signalling.
Berridge, M. J. (1997) · The Journal of Physiology · doi:10.1113/jphysiol.1997.sp021927 · Cited by 1,018
Armstrong, J. A., & Hart, P. D. (1971) · The Journal of Experimental Medicine · doi:10.1084/jem.134.3.713 · Cited by 990
Cited by
Gutierrez, M. G., Master, S. S., Singh, S. B., Taylor, G. A., Colombo, M. I., & Deretic, V. (2004) · Cell · doi:10.1016/j.cell.2004.11.038 · Cited by 2,252
Mycobacterium tuberculosis Pathogenesis and Molecular Determinants of Virulence
Smith, I. (2003) · Clinical Microbiology Reviews · doi:10.1128/cmr.16.3.463-496.2003 · Cited by 1,154
Phagocytosis of Microbes: Complexity in Action
Underhill, D. M., & Ozinsky, A. (2002) · Annual Review of Immunology · doi:10.1146/annurev.immunol.20.103001.114744 · Cited by 1,124
Survival of Mycobacteria in Macrophages Is Mediated by Coronin 1-Dependent Activation of Calcineurin
Jayachandran, R., Sundaramurthy, V., Combaluzier, B., Mueller, P., Korf, H., Huygen, K., Miyazaki, T., Albrecht, I., Massner, J., & Pieters, J. (2007) · Cell · doi:10.1016/j.cell.2007.04.043 · Cited by 310
Kyrmizi, I., Ferreira, H., Carvalho, A., Figueroa, J. A. L., Zarmpas, P., Cunha, C., Akoumianaki, T., Stylianou, K., Deepe, G. S., Samonis, G., et al. (2018) · Nature Microbiology · doi:10.1038/s41564-018-0167-x · Cited by 96
Metadata
- Authors
- Malik, Z. A., Denning, G. M., & Kusner, D. J.
- Venue
- The Journal of Experimental Medicine
- Publisher
- Rockefeller University Press
- Published
- 2000-01-17
- Volume
- 191
- Issue
- 2
- Pages
- 287-302
- ISSN
- 0022-1007 (ISSN-L)
- 1540-9538
- OpenAlex
- W2158155888
- Cited by
- 331
- References
- 60
- FWCI
- 11.91
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Malik, Z. A., Denning, G. M., & Kusner, D. J. (2000). Inhibition of Ca2+ Signaling by Mycobacterium tuberculosis Is Associated with Reduced Phagosome–Lysosome Fusion and Increased Survival within Human Macrophages. The Journal of Experimental Medicine. https://doi.org/10.1084/jem.191.2.287
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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