The DOI matches its Crossref record; 68 of the 69 references it cites were found in a registry or an index.
Requirement for Ras Guanine Nucleotide Releasing Protein 3 in Coupling Phospholipase C-γ2 to Ras in B Cell Receptor Signaling
Open accessAbstract
Two important Ras guanine nucleotide exchange factors, Son of sevenless (Sos) and Ras guanine nucleotide releasing protein (RasGRP), have been implicated in controlling Ras activation when cell surface receptors are stimulated. To address the specificity or redundancy of these exchange factors, we have generated Sos1/Sos2 double- or RasGRP3-deficient B cell lines and determined their ability to mediate Ras activation upon B cell receptor (BCR) stimulation. The BCR requires RasGRP3; in contrast, epidermal growth factor receptor is dependent on Sos1 and Sos2. Furthermore, we show that BCR-induced recruitment of RasGRP3 to the membrane and the subsequent Ras activation are significantly attenuated in phospholipase C-γ2–deficient B cells. This defective Ras activation is suppressed by the expression of RasGRP3 as a membrane-attached form, suggesting that phospholipase C-γ2 regulates RasGRP3 localization and thereby Ras activation.
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“Several biochemical pathways and processes were shown to negatively regulate PLCγ2 activity and the resulting calcium flux after BCR engagement. These include RasGRP3 (39, 40), Bam32 (41), hematopoietic adaptor protein Dok-3 (downstream of kinase-3) with Grb2 (42), Themis2, an adaptor protein with no known enzymatic activity, via constitutive binding to Lyn, Grb2, and PLCγ2 to mediate its function”
From The role of PLCγ2 in immunological disorders, cancer, and neurodegeneration (2021) · cites this paper as [39]
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All ras proteins are polyisoprenylated but only some are palmitoylated
Hancock, J. F., Magee, A. I., Childs, J. E., & Marshall, C. J. (1989) · Cell · doi:10.1016/0092-8674(89)90054-8 · Cited by 1,775
Clonal selection and learning in the antibody system
Rajewsky, K. (1996) · Nature · doi:10.1038/381751a0 · Cited by 1,701
Egan, S. E., Giddings, B. W., Brooks, M. W., Buday, L., Sizeland, A. M., & Weinberg, R. A. (1993) · Nature · doi:10.1038/363045a0 · Cited by 1,200
Increasing complexity of Ras signaling
Campbell, S. L., Khosravi-Far, R., Rossman, K. L., Clark, G. J., & Der, C. J. (1998) · Oncogene · doi:10.1038/sj.onc.1202174 · Cited by 1,079
Buday, L., & Downward, J. (1993) · Cell · doi:10.1016/0092-8674(93)90146-h · Cited by 1,064
Hancock, J. F., Paterson, H., & Marshall, C. J. (1990) · Cell · doi:10.1016/0092-8674(90)90294-o · Cited by 1,042
Li, N., Batzer, A., Daly, R. J., Yajnik, V., Skolnik, E. Y., Chardin, P., Bar‐Sagi, D., Margolis, B. L., & Schlessinger, J. (1993) · Nature · doi:10.1038/363085a0 · Cited by 938
Ras proteins: different signals from different locations
Hancock, J. F. (2003) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1105 · Cited by 856
Stimulation of p21ras upon T-cell activation
Downward, J., Graves, J. D., Warne, P. H., Rayter, S., & Cantrell, D. A. (1990) · Nature · doi:10.1038/346719a0 · Cited by 841
Bar‐Sagi, D., & Hall, A. (2000) · Cell · doi:10.1016/s0092-8674(00)00115-x · Cited by 800
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- 1992, Mol. Cell. Biol., 12, 3305
- 2002, Nat. Rev. Mol. Cell Biol., 3, 339
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Neumann, K. (2008) · PUB – Publications at Bielefeld University (Bielefeld University) · Cited by 1
Stevens, R. L., Yang, Y., & Li, L. (2004) · Clinical & Experimental Allergy Reviews · doi:10.1111/j.1472-9725.2004.00042.x · Cited by 1
Brezski, R. J. (2007) · Scholarly Commons (University of Pennsylvania)
Grabbe, A. (2005) · PUB – Publications at Bielefeld University (Bielefeld University)
Ligationbut Not T Cells, to Antigen Receptor 3BP2 Deficiency Impairs the Response of B
de la Fuente, M. Á., Kumar, L., Lu, B., & Raif, S. G. (2013)
Szatkowski, D. (2007) · DuEPublico (University of Duisburg-Essen)
Signal Transduction by the B Cell Antigen Receptor
Engels, N., & Wienands, J. (2016) · Elsevier eBooks · doi:10.1016/b978-0-12-374279-7.11001-x
Perova, T. (2014) · TSpace (University of Toronto)
Regulatory roles of PI3Ks and PH domain-containing adaptor protein Bam32 in humoral immune responses
Zhang, T. (2010) · Mspace (University of Manitoba)
Untersuchung der Rolle von BRD7 und DOT1L im Prä-B-Zellrezeptor-Signalweg
Peter, J. L. (2023) · Publication Server of Goethe University Frankfurt am Main (Goethe University Frankfurt) · doi:10.21248/gups.73382
Signal Transduction by the B Cell Antigen Receptor
Engels, N., & Wienands, J. (2025) · Elsevier eBooks · doi:10.1016/b978-0-12-824465-4.00179-4
Xie, J., Zhang, S., Lee, W., Tao, H., Pan, Y., Luo, X., & Zhong, X. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.10.05.756823
Li, X., Duan, X., Liu, N., Tang, W., He, Y., Zhou, D., Yan, J., & Zhou, Y. (2026) · Cell Biochemistry and Function · doi:10.1002/cbf.70315
Metadata
- Authors
- Oh-hora, M., Johmura, S., Hashimoto, A., Hikida, M., & Kurosaki, T.
- Affiliations
- 1Department of Molecular Genetics, Institute for Liver Research, Kansai Medical University, Moriguchi 570-8506, Japan
- Kansai Medical University (JP)
- Venue
- The Journal of Experimental Medicine
- Publisher
- Rockefeller University Press
- Published
- 2003-12-15
- Volume
- 198
- Issue
- 12
- Pages
- 1841-1851
- ISSN
- 0022-1007 (ISSN-L)
- 1540-9538
- PMC
- PMC2194160
- OpenAlex
- W2131566341
- Cited by
- 113
- References
- 69
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 2 · 2023: 2 · 2022: 2 · 2021: 2 · 2020: 4 · 2019: 4 · 2018: 1 · 2017: 2 · 2016: 4 · 2015: 4 · 2014: 3 · 2013: 6 · 2012: 4
- FWCI
- 3.57
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by-nc-sa
- Locations
- The Journal of Experimental Medicine (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Funders
- Uehara Memorial Foundation
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Tyrosine Phosphatases
- Keywords
- RasGRP3, SOS1, SOS2, Ras guanine nucleotide exchange factor, Ras activation, B cell receptor signaling, PLCγ2, epidermal growth factor receptor, membrane recruitment, B-cell lines
- MeSH terms
- Amino Acid Sequence, Animals, Mice, Inbred C57BL, Molecular Sequence Data, Type C Phospholipases, Receptors, Antigen, B-Cell, Signal Transduction, ras Proteins, Guanine Nucleotide Exchange Factors, ras Guanine Nucleotide Exchange Factors, Son of Sevenless Proteins, SOS1 Protein, Mice, Phospholipase C gamma
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