The DOI matches its Crossref record; 51 of the 51 references it cites were found in a registry or an index.
DC-SIGN–mediated Infectious Synapse Formation Enhances X4 HIV-1 Transmission from Dendritic Cells to T Cells
Open accessAbstract
Dendritic cells (DCs) are essential for the early events of human immunodeficiency virus (HIV) infection. Model systems of HIV sexual transmission have shown that DCs expressing the DC-specific C-type lectin DC-SIGN capture and internalize HIV at mucosal surfaces and efficiently transfer HIV to CD4+ T cells in lymph nodes, where viral replication occurs. Upon DC–T cell clustering, internalized HIV accumulates on the DC side at the contact zone (infectious synapse), between DCs and T cells, whereas HIV receptors and coreceptors are enriched on the T cell side. Viral concentration at the infectious synapse may explain, at least in part, why DC transmission of HIV to T cells is so efficient. Here, we have investigated the role of DC-SIGN on primary DCs in X4 HIV-1 capture and transmission using small interfering RNA–expressing lentiviral vectors to specifically knockdown DC-SIGN. We demonstrate that DC-SIGN− DCs internalize X4 HIV-1 as well as DC-SIGN+ DCs, although binding of virions is reduced. Strikingly, DC-SIGN knockdown in DCs selectively impairs infectious synapse formation between DCs and resting CD4+ T cells, but does not prevent the formation of DC–T cells conjugates. Our results demonstrate that DC-SIGN is required downstream from viral capture for the formation of the infectious synapse between DCs and T cells. These findings provide a novel explanation for the role of DC-SIGN in the transfer and enhancement of HIV infection from DCs to T cells, a crucial step for HIV transmission and pathogenesis.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The cited source says what the claim attributes to it.
In the citing paper
“However, the results of other studies indicate the existence of a DC-SIGN-independent mechanism of trans-infection with HIV-1 viral particles [81,82,83,84].”
From HIV-1 and HTLV-1 Transmission Modes: Mechanisms and Importance for Virus Spread (2022) · cites this paper as [81]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells
Geijtenbeek, T. B. H., Kwon, D. S., Torensma, R., van Vliet, S. J., van Duijnhoven, G. C. F., Middel, J., Cornelissen, I. L. M. H. A., Nottet, H. S. L. M., KewalRamani, V. N., Littman, D. R., et al. (2000) · Cell · doi:10.1016/s0092-8674(00)80694-7 · Cited by 2,447
Self-citationMultiply attenuated lentiviral vector achieves efficient gene delivery in vivo
Zufferey, R., Nagy, D., Mandel, R. J., Naldini, L., & Trono, D. (1997) · Nature Biotechnology · doi:10.1038/nbt0997-871 · Cited by 1,977
T-cell priming by dendritic cells in lymph nodes occurs in three distinct phases
Mempel, T. R., Henrickson, S. E., & von Andrian, U. H. (2004) · Nature · doi:10.1038/nature02238 · Cited by 1,720
Geijtenbeek, T. B. H., Torensma, R., van Vliet, S. J., van Duijnhoven, G. C. F., Adema, G. J., van Kooyk, Y., & Figdor, C. G. (2000) · Cell · doi:10.1016/s0092-8674(00)80693-5 · Cited by 1,701
Self-citationCollaborative Induction of Inflammatory Responses by Dectin-1 and Toll-like Receptor 2
Gantner, B. N., Simmons, R. M., Canavera, S. J., Akira, S., & Underhill, D. M. (2003) · The Journal of Experimental Medicine · doi:10.1084/jem.20021787 · Cited by 1,682
Mycobacteria Target DC-SIGN to Suppress Dendritic Cell Function
Geijtenbeek, T. B. H., van Vliet, S. J., Koppel, E. A., Sánchez‐Hernández, M., Vandenbroucke‐Grauls, C. M. J. E., Appelmelk, B. J., & van Kooyk, Y. (2002) · The Journal of Experimental Medicine · doi:10.1084/jem.20021229 · Cited by 1,079
Self-citationSpread of HTLV-I Between Lymphocytes by Virus-Induced Polarization of the Cytoskeleton
Igakura, T., Stinchcombe, J. C., Goon, P. K. C., Taylor, G. P., Weber, J. N., Griffiths, G. M., Tanaka, Y., Osame, M., & Bangham, C. R. M. (2003) · Science · doi:10.1126/science.1080115 · Cited by 755
Recruitment of HIV and Its Receptors to Dendritic Cell-T Cell Junctions
McDonald, D., Wu, L., Bohks, S. M., KewalRamani, V. N., Unutmaz, D., & Hope, T. J. (2003) · Science · doi:10.1126/science.1084238 · Cited by 707
Engering, A. J., Geijtenbeek, T. B. H., van Vliet, S. J., Wijers, M., van Liempt, E., Demaurex, N., Lanzavecchia, A., Fransen, J. A. M., Figdor, C. G., Piguet, V., et al. (2002) · The Journal of Immunology · doi:10.4049/jimmunol.168.5.2118 · Cited by 630
Self-citationHIV-1 Cell to Cell Transfer across an Env-induced, Actin-dependent Synapse
Jolly, C., Kashefi, K., Hollinshead, M., & Sattentau, Q. J. (2004) · The Journal of Experimental Medicine · doi:10.1084/jem.20030648 · Cited by 623
Flagged first, then by citations
Research works printed but not linked: 3
- 2003, Curr. Top. Microbiol. Immunol., 276, 1
- 2003, Curr. Top. Microbiol. Immunol., 276, 31
- 2001, Nat. Immunol., 2, 925
Cited by
Sorted by citations
Items 201–240 of 240
Deka, H., Ghosh, A., & Baishya, D. (2025) · Glycoconjugate Journal · doi:10.1007/s10719-025-10179-w · Cited by 1
Innate Recognition of HIV-1 Glycans: Implications for Infection, Transmission, and Immunity
van der Aar, A. M. G., Gringhuis, S. I., & Geijtenbeek, T. B. H. (2013) · Springer eBooks · doi:10.1007/978-1-4614-8872-9_2 · Cited by 1
Self-citationMucosal Dendritic Cells in HIV-1 Susceptibility: A Critical Role for C-type Lectin Receptors
Hertoghs, N., van Pul, L., & Geijtenbeek, T. B. H. (2017) · Future Virology · doi:10.2217/fvl-2017-0020 · Cited by 1
Self-citationAcquired Immune Deficiency Syndrome
Khan, M. M. (2016) · Springer eBooks · doi:10.1007/978-3-319-30273-7_9 · Cited by 1
Lectin-like interactions in virus–cell recognition
Steffen, I., Tsegaye, T. S., & Pöhlmann, S. (2010) · Elsevier eBooks · doi:10.1016/b978-0-12-374546-0.00028-6 · Cited by 1
Stochastic Model for Langerhans Cells and HIV Dynamics in VivoPreprint
Mbogo, R. W., Luboobi, L. S., & Odhiambo, J. (2013) · SSRN Electronic Journal · doi:10.2139/ssrn.2324112 · Cited by 1
Interactions of Hemorrhagic Fever Viruses with Dendritic Cells
Pöhlmann, S. (2006) · Wiley eBooks · doi:10.1002/9783527619696.ch40
Almeida, R. R. (2014) · Digital Library of Theses and Dissertations (Universidade de São Paulo) · doi:10.11606/t.5.2014.tde-29102014-160344
Papel de la quimiocina CXCL12/SDF-1 en la evolución del tropismo viral en la infección por el VIH-1
Fernández, N. G. (2014) · LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas)
UC781: BETA-CYCLODEXTRIN COMPLEXATION AND FORMULATION AS AN ANTI-HIV MICROBICIDE
Yang, H. (2008) · D-Scholarship@Pitt (University of Pittsburgh)
Seeking a mechanism of action for the anti-HIV properties of the CD8 antiviral factor, CAF
Shridhar, V. (2011) · D-Scholarship@Pitt (University of Pittsburgh)
Chemokine Receptors and HIV/AIDS
Stantchev, T. S., & Broder, C. C. (2007) · Humana Press eBooks · doi:10.1007/978-1-59745-020-1_13
Characterisation of llama antibody fragments able to act as HIV-1 entry inhibitors
Quigley, A. F. (2009) · OpenGrey (Institut de l'Information Scientifique et Technique)
Piguet, V., Garcia, E., Pion, M., Arrighi, J.-F., Abraham, Blot, G., Pelchen–Matthews, A., Collinson, L., Marsh, M., & Piguet, V. (2005) · Annales de Dermatologie et de Vénéréologie · doi:10.1016/s0151-9638(05)79624-1
Self-citationDendritic Cell-Mediated Viral Transmission: A Potential Drug Target?
de Witte, L. D., van Kooyk, Y., & Geijtenbeek, T. B. H. (2006) · Future Virology · doi:10.2217/17460794.1.5.615
Self-citationLlewellyn, G. N. (2012) · Deep Blue (University of Michigan)
Almeida, R. R. (2011) · Digital Library of Theses and Dissertations (Universidade de São Paulo) · doi:10.11606/d.5.2011.tde-27072011-174551
[HIV1 Env N-glycans and their interactions with the immune system].
Reynard, F., Bedin, F., & Verrier, B. (2006) · PubMed · doi:10.1684/vir.2011.4242
Affecting Langerhans Cell Function Enhances HIV-1 Susceptibility by Herpes Simplex Virus Type 2
Geijtenbeek, T. B. H., & de Jong, M. A. W. P. (2010)
Self-citationHealth Sciences Research Commons
Cuadrado, E., van Hamme, J. L., & van Dort, K. A. (2012)
Fairman, P. (2013) · uO Research (University of Ottawa) · doi:10.20381/ruor-2947
Gray, C. M., Gumbi, P. P., Zembe, L., Radebe, M., & Walker, B. D. (2012) · Elsevier eBooks · doi:10.1016/b978-1-4557-0695-2.00004-3
Implication de DC-SIGN et DC-SIGNR dans la transmission mère-enfant du VIH-1
Boily-Larouche, G. (2012) · doi:10.71781/25869
Induction of Autophagy in HIV-1-Uninfected Cells
Espert, L., & Biard‐Piechaczyk, M. (2013) · Elsevier eBooks · doi:10.1016/b978-0-12-405877-4.00007-x
Gray, C. M., & Walker, B. D. (2008) · Elsevier eBooks · doi:10.1016/b978-1-4160-2882-6.50008-3
Bedeutung zellulärer Anheftungsfaktoren für die Ausbreitung des humanen Immundefizienz-Virus
Chaipan, C. (2008) · OPUS FAU (Kooperativer Bibliotheksverbund Berlin-Brandenburg (KOBV), on behalf of the Universitätsbibliothek Erlangen-Nürnberg)
Wang, W., Truong, K., Ye, C., Sharma, S., He, H., Liu, L., Wen, M., Misra, A., Zhou, P., & Kimata, J. T. (2023) · Frontiers in Immunology · doi:10.3389/fimmu.2023.1167965 · Cited by 1
Janevska, M., Witkowski, W., Vermeíre, J., Borowicz, M., Naessens, E., Vanderstraeten, H., Nauwynck, H., Favoreel, H. W., & Verhasselt, B. (2025) · Journal of Virology · doi:10.1128/jvi.02066-24
Immune reconstitution in paediatric patients with acquired immunodeficiency
Pensieroso, S. (2008) · Cineca Institutional Research Information System (Tor Vergata University)
Caractérisation des activités antivirales des anticorps anti-VIH-1 et anti-SARS-CoV-2
Dufloo, J. (2021) · theses.fr (ABES) · doi:10.70675/215798eaz7f82z4e57za8eazf4453d37b8c8
Bonaventure, B. (2020) · theses.fr (ABES) · doi:10.70675/768c3190z3177z4b25z88b9z9a029e425462
Etude de la transmission intercellulaire du VIH-1 vers les macrophages par fusion cellule-cellule
Mascarau, R. (2022) · theses.fr (ABES) · doi:10.70675/2e3aacaazcd4fz49a5zb350zcd4933447d78
C-Type Lectins: Multifaceted Receptors in Phagocyte Biology
Cambi, A., & Figdor, C. G. (2014) · ASM Press eBooks · doi:10.1128/9781555816650.ch7
Metadata
- Authors
- Arrighi, J.-F., Pion, M., Garcia, E., Escola, J.-M., van Kooyk, Y., Geijtenbeek, T. B., & Piguet, V.
- Affiliations
- University Hospital of Geneva (CH)
- Amsterdam UMC Location Vrije Universiteit Amsterdam (NL)
- Corresponding author
- Piguet, Vincent
- Venue
- The Journal of Experimental Medicine
- Publisher
- Rockefeller University Press
- Published
- 2004-11-15
- Volume
- 200
- Issue
- 10
- Pages
- 1279-1288
- ISSN
- 0022-1007 (ISSN-L)
- 1540-9538
- PMC
- PMC2211914
- OpenAlex
- W2155011421
- Cited by
- 240
- References
- 51
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 1 · 2023: 4 · 2022: 4 · 2021: 4 · 2020: 3 · 2019: 4 · 2018: 7 · 2017: 5 · 2016: 6 · 2015: 11 · 2014: 14 · 2013: 11 · 2012: 20
- FWCI
- 7.55
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by-nc-sa
- Locations
- The Journal of Experimental Medicine (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Amsterdam UMC (VU Amsterdam) - Institutional Repository (publishedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Pure Amsterdam UMC (submittedVersion)
- Funders
- National Science Foundation
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (67200, 68785)
- Topics
- Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
- Health Sciences › Medicine › Infectious Diseases › HIV/AIDS Research and Interventions
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Keywords
- DC-SIGN, X4 HIV-1, dendritic cells, HIV transmission, CD4+ T cells, C-type lectin, viral capture, HIV co-receptor, HIV pathogenesis, mucosal HIV transmission
- MeSH terms
- DC-Specific ICAM-3 Grabbing Nonintegrin, Antibodies, Monoclonal, Binding Sites, Cells, Cultured, Dendritic Cells, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Genetic Vectors, Humans, Microscopy, Fluorescence, Receptors, Cell Surface, T-Lymphocytes, HIV-1, HIV Infections, Cell Adhesion Molecules, Lentivirus, RNA, Small Interfering, Lectins, C-Type
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
