Recruitment of HIV and Its Receptors to Dendritic Cell-T Cell Junctions
Abstract
Monocyte-derived dendritic cells (MDDCs) can efficiently bind and transfer HIV infectivity without themselves becoming infected. Using live-cell microscopy, we found that HIV was recruited to sites of cell contact in MDDCs. Analysis of conjugates between MDDCs and T cells revealed that, in the absence of antigen-specific signaling, the HIV receptors CD4, CCR5, and CXCR4 on the T cell were recruited to the interface while the MDDCs concentrated HIV to the same region. We propose that contact between dendritic cells and T cells facilitates transmission of HIV by locally concentrating virus, receptor, and coreceptor during the formation of an infectious synapse.
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Within a few minutes after formation of cell conjugates, CD4, CCR5, CXCR4, and HIV-1 accumulate at intercellular contact sites, and captured HIV-1 particles are located on or near the MDDC surface.
“Within a few minutes after the formation of the cell conjugates, the accumulation of CD4, CCR5, CXCR4, and HIV-1 was observed at the intercellular contact sites, and the captured HIV-1 particles were located on or near the MDDC surface [89].”
From HIV-1 and HTLV-1 Transmission Modes: Mechanisms and Importance for Virus Spread (2022) · cites this paper as [89]
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“Within a few minutes after the formation of the cell conjugates, the accumulation of CD4, CCR5, CXCR4, and HIV-1 was observed at the intercellular contact sites, and the captured HIV-1 particles were located on or near the MDDC surface [89].”
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- Authors
- McDonald, D., Wu, L., Bohks, S. M., KewalRamani, V. N., Unutmaz, D., & Hope, T. J.
- Affiliations
- Vanderbilt University (US)
- Corresponding author
- Hope, Thomas J.
- Venue
- Science
- Published
- 2003-05-23
- Volume
- 300
- Issue
- 5623
- Pages
- 1295-1297
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2083889661
- Cited by
- 707
- References
- 1
- Citations by year
- 2026: 3 · 2025: 8 · 2024: 8 · 2023: 15 · 2022: 19 · 2021: 12 · 2020: 20 · 2019: 20 · 2018: 22 · 2017: 18 · 2016: 20 · 2015: 27 · 2014: 33 · 2013: 46 · 2012: 45
- FWCI
- 18.72
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Allergy and Infectious Diseases (R01AI052051, R01 AI49131, R01AI049131, R01-AI47770, R01AI047770, R01-AI52051)
- Topics
- Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
- Health Sciences › Medicine › Infectious Diseases › HIV/AIDS Research and Interventions
- Health Sciences › Medicine › Oncology › Chemokine receptors and signaling
- Keywords
- HIV transmission, dendritic cells, monocyte-derived dendritic cells, HIV receptor, CD4, CCR5, CXCR4, viral coreceptor
- MeSH terms
- Cell Adhesion, Cell Membrane, Cells, Cultured, Dendritic Cells, Fluorescent Antibody Technique, Humans, Intercellular Junctions, Lipopolysaccharides, Luciferases, Luminescent Proteins, Models, Biological, Monocytes, Virion, CD4-Positive T-Lymphocytes, HIV-1, CD4 Antigens, Receptors, HIV, Gene Products, vpr, Coculture Techniques, Receptors, CCR5, Receptors, CXCR4, Green Fluorescent Proteins, vpr Gene Products, Human Immunodeficiency Virus
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