PROTEIN COAGULATION AND ITS REVERSAL
Open accessAbstract
1. By a procedure involving the use of acid acetone hemoglobin may be rapidly separated into a precipitate of denatured globin and an acetone solution of heme. 2. By neutralization procedures the denatured globin may be largely converted into a soluble, apparently native form which can combine with heme to form hemoglobin. 3. The heme may be obtained in acetone-free, slightly alkaline solution without the use of strong alkali which may modify the heme.
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The folding paradigm stipulated that unfolded molecules spontaneously fold into the same shape, with the linear amino acid sequence specifying the folded native state.
“The folding paradigm stipulated that unfolded molecules will always spontaneously fold into the same shape; that is, the linear amino acid sequence specifies a protein’s folded native state.”
From AlphaFold, Artificial Intelligence (AI), and Allostery (2022) · cites this paper as [142]
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- Authors
- Anson, M. L., & Mirsky, A. E.
- Affiliations
- Rockefeller University Hospital (US)
- Venue
- Journal of General Physiology
- Publisher
- Rockefeller University Press
- Published
- 1930-03-20
- Volume
- 13
- Issue
- 4
- Pages
- 469-476
- ISSN
- 0022-1295 (ISSN-L)
- 1540-7748
- PMC
- PMC2141062
- OpenAlex
- W2125550241
- Cited by
- 497
- References
- 10
- Citations by year
- 2024: 1 · 2023: 1 · 2022: 2 · 2021: 1 · 2020: 1 · 2019: 1 · 2017: 2 · 2016: 1 · 2015: 1 · 2014: 3 · 2013: 1
- FWCI
- 10.60
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by-nc-sa
- Locations
- The Journal of General Physiology (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Hemoglobin structure and function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein purification and stability
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Blood properties and coagulation
- Keywords
- protein coagulation, protein denaturation, hemoglobin, globin, heme
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