The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
SaeR Binds a Consensus Sequence within Virulence Gene Promoters to Advance USA300 Pathogenesis
Open accessAbstract
This investigation examines the role of the SaeR/S 2-component system in USA300, a prominent circulating clone of community-associated methicillin-resistant Staphylococcus aureus. Using a saeR/S isogenic deletion mutant of USA300 (USA300DeltasaeR/S) in murine models of sepsis and soft-tissue infection revealed that this sensory system is critical to pathogenesis of USA300 during both superficial and invasive infection. Oligonucleotide microarray and real-time reverse-transcriptase polymerase chain reaction identified numerous extracellular virulence genes that are down-regulated in USA300DeltasaeR/S. Unexpectedly, an up-regulation of mecA and mecR1 corresponded to increased methicillin resistance in USA300DeltasaeR/S. 5'-RACE analysis defined transcript start sites for sbi, efb, mecA, lukS-PV, hlb, SAUSA300_1975, and hla, to underscore a conserved consensus sequence within promoter regions of genes under strong SaeR/S transcriptional regulation. Electrophoretic mobility shift assay experiments illustrated direct binding of SaeR(His) to promoter regions containing the conserved consensus sequence. Collectively, the findings of this investigation demonstrate that SaeR/S directly interacts with virulence gene promoters to significantly influence USA300 pathogenesis.
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Klevens, R. M. (2007) · JAMA · doi:10.1001/jama.298.15.1763 · Cited by 3,726
Miller, L. G., Perdreau-Remington, F., Rieg, G., Mehdi, S., Perlroth, J., Bayer, A. S., Tang, A. W., Phung, T. O., & Spellberg, B. (2005) · New England Journal of Medicine · doi:10.1056/nejmoa042683 · Cited by 1,053
Wang, R., Braughton, K. R., Kretschmer, D., Bach, T.-H. L., Queck, S. Y., Li, M., Kennedy, A. D., Dorward, D. W., Klebanoff, S. J., Peschel, A., et al. (2007) · Nature Medicine · doi:10.1038/nm1656 · Cited by 1,046
Insights into Mechanisms Used by Staphylococcus aureus to Avoid Destruction by Human Neutrophils
Voyich, J. M., Braughton, K. R., Sturdevant, D. E., Whitney, A. R., Saïd-Salim, B., Porcella, S. F., Long, R. D., Dorward, D. W., Gardner, D. J., Kreiswirth, B. N., et al. (2005) · The Journal of Immunology · doi:10.4049/jimmunol.175.6.3907 · Cited by 563
Self-citationEvolution of virulence in epidemic community-associated methicillin-resistant Staphylococcus aureus
Li, M., Diep, B. A., Villaruz, A. E., Braughton, K. R., Jiang, X., DeLeo, F. R., Chambers, H. F., Lu, Y., & Otto, M. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0900743106 · Cited by 394 · cited 1×
“common genes between strains [41], we verified the importance”
BackgroundWardenburg, J. B., Palazzolo‐Ballance, A. M., Otto, M., Schneewind, O., & DeLeo, F. R. (2008) · The Journal of Infectious Diseases · doi:10.1086/592053 · Cited by 236
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The Bicomponent Pore-Forming Leucocidins of Staphylococcus aureus
Alonzo, F., & Torres, V. J. (2014) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00055-13 · Cited by 276
Kiedrowski, M. R., Kavanaugh, J. S., Malone, C. L., Mootz, J. M., Voyich, J. M., Smeltzer, M. S., Bayles, K. W., & Horswill, A. R. (2011) · PLoS ONE · doi:10.1371/journal.pone.0026714 · Cited by 268
Self-citationCodY, a master integrator of metabolism and virulence in Gram-positive bacteria
Brinsmade, S. R. (2017) · Current Genetics · doi:10.1007/s00294-016-0656-5 · Cited by 89
Metadata
- Authors
- Nygaard, T. K., Pallister, K. B., Ruzevich, P., Griffith, S., Vuong, C., & Voyich, J. M.
- Venue
- The Journal of Infectious Diseases
- Publisher
- Oxford University Press (OUP)
- Published
- 2010-01-15
- Volume
- 201
- Issue
- 2
- Pages
- 241-254
- ISSN
- 0022-1899 (ISSN-L)
- 1537-6613
- OpenAlex
- W1968319760
- Cited by
- 162
- References
- 6
- FWCI
- 6.07
- Type
- journal article
- Language
- en
- Open access
- green
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- Nygaard, T. K., Pallister, K. B., Ruzevich, P., Griffith, S., Vuong, C., & Voyich, J. M. (2010). SaeR Binds a Consensus Sequence within Virulence Gene Promoters to Advance USA300 Pathogenesis. The Journal of Infectious Diseases. https://doi.org/10.1086/649570
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