Cited by 275Antimicrobial Resistance in StaphylococcusBacterial biofilms and quorum sensingBacterial Genetics and Biotechnology

Staphylococcus aureus Small‐Colony Variants Are Adapted Phenotypes for Intracellular Persistence

Tuchscherr, Lorena, Heitmann, Vanessa, Hussain, Muzaffar, Viemann, Dorothee, Roth, Johannes, von Eiff, Christof, Peters, Georg, Becker, Karsten +1 more·2010·The Journal of Infectious Diseases

Abstract

BACKGROUND: Staphylococcus aureus is an important human pathogen of endovascular diseases, which can take a chronic course with a high relapse rate despite antimicrobial treatment. Thus far, persistent and antibiotic-refractory infections have been largely associated with a subpopulation of S. aureus, the small-colony variants (SCVs). METHODS: In this work, we used endothelial cells to investigate infection with the highly virulent wild-type isolate (6850), 2 stable isogenic SCV phenotypes (hemB mutant IIb13 and JB1), and the complemented mutant. RESULTS: All strains were highly invasive in endothelial cells but largely differed in host response induction. Microarray analysis showed that wild-type phenotypes up-regulated a large number of endothelial genes (including genes involved in innate immunity), whereas the SCVs did not cause these dramatic changes. The inflammatory response and cytotoxicity were strongest shortly after infection and largely decreased within the following days, which was accompanied by a fast elimination of intracellular wild-type bacteria. By contrast, SCVs survived within endothelial cells at high numbers. CONCLUSION: S. aureus intracellular persistence via the development of an adapted subpopulation of SCVs most likely represents an important strategy of S. aureus to hide within the host cells, which could be a reservoir for chronic infections.

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Crossref · 10.1086/656047

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Authors
Tuchscherr, L., Heitmann, V., Hussain, M., Viemann, D., Roth, J., von Eiff, C., Peters, G., Becker, K., & Löffler, B.
Affiliations
University Hospital Münster (DE)
Venue
The Journal of Infectious Diseases
Published
2010-10
Volume
202
Issue
7
Pages
1031-1040
ISSN
  • 0022-1899 (ISSN-L)
  • 1537-6613
OpenAlex
W2134765452
Cited by
275
References
36
Citations by year
2026: 13 · 2025: 16 · 2024: 17 · 2023: 20 · 2022: 16 · 2021: 23 · 2020: 15 · 2019: 19 · 2018: 16 · 2017: 16 · 2016: 15 · 2015: 23 · 2014: 17 · 2013: 21 · 2012: 13
FWCI
10.06
Type
journal article
Language
en
Open access
closed
Locations
  • The Journal of Infectious Diseases (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
Staphylococcus aureus, small colony variants, intracellular persistence, endothelial cells, host response, innate immunity, bacterial invasion, chronic infection, antibiotic-refractory infection, endovascular infection, cytotoxicity, inflammation
MeSH terms
Cells, Cultured, Cytoplasm, Humans, Staphylococcus aureus, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, Endothelial Cells, Host-Pathogen Interactions

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