Detection of Staphylococcus aureus by 16S rRNA directed in situ hybridisation in a patient with a brain abscess caused by small colony variants
Open accessAbstract
A 45 year old man was admitted to hospital with a right sided facial paralysis and three month history of seizures. Computed tomography showed a left temporal mass including both intracerebral and extracerebral structures. Ten years earlier the patient had undergone a neurosurgical intervention in the same anatomical region to treat a subarachnoid haemorrhage. In tissue samples and pus obtained during neurosurgery, Staphylococcus aureus was detected by a 16S rRNA-directed in situ hybridisation technique. Following long term cultivation, small colony variants (SCV) of methicillin resistant S aureus were identified. The patient was treated successfully with a combination of vancomycin and rifampin followed by prolonged treatment with teicoplanin, with no sign of infection on follow up nine months after discharge. This is the first report in which S aureus SCV have been identified as causative organisms in a patient with brain abscess and in which in situ hybridisation has been used to detect S aureus in a clinical specimen containing SCV. Antimicrobial agents such as rifampin which have intracellular activity should be included in treatment of infections caused by S aureus SCV.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
This correlates with the clinical evidence that SCVs are able to persist in host tissues, resisting clearance by immune cells that expose the pathogen to the oxidative burst.
“This correlates with the clinical evidence that SCVs are able to persist in host tissues, resisting clearance by immune cells that expose the pathogen to the oxidative burst (1–14, 36, 37).”
From Staphylococcus aureus Adapts to Oxidative Stress by Producing H <sub>2</sub> O <sub>2</sub> -Resistant Small-Colony Variants via the SOS Response (2015) · cites this paper as [7]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Identification of methicillin-resistant strains of staphylococci by polymerase chain reaction
Murakami, K., Minamide, W., Wada, K., Nakamura, E., Teraoka, H., & Watanabe, S. (1991) · Journal of Clinical Microbiology · doi:10.1128/jcm.29.10.2240-2244.1991 · Cited by 939 · see citing works
Persistent and Relapsing Infections Associated with Small-Colony Variants of Staphylococcus aureus
Proctor, R. A., van Langevelde, P., Kristjansson, M., Maslow, J. N., & Arbeit, R. D. (1995) · Clinical Infectious Diseases · doi:10.1093/clinids/20.1.95 · Cited by 437 · see citing works
Kahl, B., Herrmann, M., Everding, A. S., Koch, H. G., Becker, K., Harms, E., Proctor, R. A., & Peters, G. (1998) · Journal of Infectious Diseases · doi:10.1086/515238 · Cited by 357 · see citing works
von Eiff, C., Bettin, D., Proctor, R. A., Rolauffs, B., Lindner, N. J., Winkelmann, W., & Peters, G. (1997) · Clinical Infectious Diseases · doi:10.1086/516962 · Cited by 249 · see citing works
Staphylococcal Small Colony Variants Have Novel Mechanisms for Antibiotic Resistance
Proctor, R. A., Kahl, B. C., von Eiff, C., Vaudaux, P. E., Lew, D. P., & Peters, G. (1998) · Clinical Infectious Diseases · doi:10.1086/514906 · Cited by 205 · see citing works
Small Colony Variants in Staphylococcal Infections: Diagnostic and Therapeutic Implications
Proctor, R. A., & Peters, G. (1998) · Clinical Infectious Diseases · doi:10.1086/514706 · Cited by 160 · see citing works
Staphylococcus aureus small colony variants, electron transport and persistent infections
McNamara, P. J., & Proctor, R. A. (2000) · International Journal of Antimicrobial Agents · doi:10.1016/s0924-8579(99)00170-3 · Cited by 127 · see citing works
Krimmer, V., Merkert, H., von Eiff, C., Frosch, M., Eulert, J., Löhr, J. F., Hacker, J. H., & Ziebuhr, W. (1999) · Journal of Clinical Microbiology · doi:10.1128/jcm.37.8.2667-2673.1999 · Cited by 88 · see citing works
Small-colony variants of Staphylococcus aureus.
Looney, W. J. (2000) · PubMed · Cited by 39 · see citing works
von Eiff, C., Peters, G., & Proctor, R. A. (2001) · Infectious agents and pathogenesis · doi:10.1007/0-306-46848-4_2 · Cited by 3 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Proctor, R. A., von Eiff, C., Kahl, B. C., Becker, K., McNamara, P., Herrmann, M., & Peters, G. (2006) · Nature Reviews Microbiology · doi:10.1038/nrmicro1384 · Cited by 1,175 · see citing works
Tuchscherr, L., Medina, E., Hussain, M., Völker, W., Heitmann, V., Niemann, S., Holzinger, D., Roth, J., Proctor, R. A., Becker, K., et al. (2011) · EMBO Molecular Medicine · doi:10.1002/emmm.201000115 · Cited by 437 · see citing works
Kahl, B. C., Becker, K., & Löffler, B. (2016) · Clinical Microbiology Reviews · doi:10.1128/cmr.00069-15 · Cited by 325 · see citing works
Tuchscherr, L., Heitmann, V., Hussain, M., Viemann, D., Roth, J., von Eiff, C., Peters, G., Becker, K., & Löffler, B. (2010) · The Journal of Infectious Diseases · doi:10.1086/656047 · Cited by 275 · see citing works
<i>Staphylococcus aureus</i> Small Colony Variants in Prosthetic Joint Infection
Sendi, P., Rohrbach, M., Graber, P., Frei, R., Ochsner, P. E., & Zimmerli, W. (2006) · Clinical Infectious Diseases · doi:10.1086/507633 · Cited by 232 · see citing works
Garcia, L. G., Lemaire, S., Kahl, B. C., Becker, K., Proctor, R. A., Denis, O., Tulkens, P. M., & Van Bambeke, F. (2013) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dkt072 · Cited by 177 · see citing works
Painter, K. L., Strange, E., Parkhill, J., Bamford, K. B., Armstrong-James, D., & Edwards, A. M. (2015) · Infection and Immunity · doi:10.1128/iai.03016-14 · Cited by 153 · see citing works
Small colony variants (SCVs) of Staphylococcus aureus – A bacterial survival strategy
Kahl, B. C. (2014) · Infection, Genetics and Evolution · doi:10.1016/j.meegid.2013.05.016 · Cited by 118 · see citing works
- Authors
- Kipp, F.
- Affiliations
- University of Münster (DE)
- Corresponding author
- Kipp, F
- ORCID
- Kipp, F
- Venue
- Journal of Neurology, Neurosurgery & Psychiatry
- Publisher
- BMJ
- Published
- 2003-07-01
- Volume
- 74
- Issue
- 7
- Pages
- 1000-1002
- ISSN
- 0022-3050 (ISSN-L)
- PMC
- PMC1738524
- OpenAlex
- W2081183044
- Cited by
- 48
- References
- 10
- Citations by year
- 2024: 2 · 2023: 2 · 2022: 1 · 2020: 3 · 2019: 2 · 2017: 2 · 2016: 3 · 2015: 3 · 2014: 3 · 2013: 4 · 2012: 2
- FWCI
- 1.55
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Neurology Neurosurgery & Psychiatry (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
- Health Sciences › Medicine › Surgery › Infectious Diseases and Tuberculosis
- Keywords
- Staphylococcus aureus, small colony variants, brain abscess, methicillin-resistant Staphylococcus aureus, rifampicin, vancomycin, teicoplanin, facial paralysis, seizures, intracranial infection, subarachnoid hemorrhage
- MeSH terms
- Brain Abscess, Humans, Male, Middle Aged, RNA, Ribosomal, 16S, Staphylococcal Infections, Staphylococcus aureus, Methicillin Resistance, In Situ Hybridization
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
