Cited by 493D Printing in Biomedical ResearchBone Tissue Engineering MaterialsInnovations in Concrete and Construction Materials

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Bioglass incorporated methacrylated collagen bioactive ink for 3D printing of bone tissue

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Kajave, Nilabh S, Schmitt, Trevor, Nguyen, Thuy-Uyen, Gaharwar, Akhilesh K, Kishore, Vipuil·2021·Biomedical Materials

DOI 10.1088/1748-605x/abc744PMC PMC8326306

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Abstract

Bioactive three-dimensional (3D) printed scaffolds are promising candidates for bone tissue engineering (BTE) applications. Here, we introduce a bioactive ink composed of Bioglass 45S5 (BG) and methacrylated collagen (CMA) for 3D printing of biomimetic constructs that resemble the organic and inorganic composition of native bone tissue. A uniform dispersion of BG particles within the collagen network improved stability and reduced swelling of collagen hydrogels. Rheological testing showed significant improvement in the yield stress and percent recovery of 3D printed constructs upon BG incorporation. Further, addition of BG improved the bone bioactivity of 3D printed constructs in stimulated body fluid. BG incorporated CMA (BG-CMA) constructs maintained high cell viability and enhanced alkaline phosphatase activity of human mesenchymal stem cells. In addition, cell-mediated calcium deposition was significantly higher on BG-CMA constructs, compared to CMA alone. In conclusion, 3D printed BG-CMA constructs have significant potential for use in BTE applications.

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Authors: Kajave, N. S., Schmitt, T., Nguyen, T.-U., Gaharwar, A. K., & Kishore, V.
Affiliations: Florida Institute of Technology (US)
Texas A&M University (US)
Corresponding author: Kishore, Vipuil
ORCID: Nguyen, Thuy-Uyen
Gaharwar, Akhilesh K
Kishore, Vipuil
Venue: Biomedical Materials
Publisher: IOP Publishing
Published: 2021-02-26
Volume: 16
Issue: 3
Pages: 035003
ISSN: 1748-6041 (ISSN-L)
1748-605X
DOI: 10.1088/1748-605x/abc744
PMC: PMC8326306
OpenAlex: W3096856907
Cited by: 46
References: 53
Citations by year: 2026: 6 · 2025: 9 · 2024: 8 · 2023: 8 · 2022: 9 · 2021: 6
FWCI: 1.81
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: publishingsupport.iopscience.iop.org/iop-standard/v1 (vor)
iopscience.iop.org/info/page/text-and-data-mining (tdm)
Locations: Biomedical Materials (publishedVersion)
PubMed (publishedVersion)
PubMed Central (acceptedVersion)
Funders: Center for Scientific Review (1R15AR071102)
Topics: Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
Physical Sciences › Engineering › Building and Construction › Innovations in Concrete and Construction Materials
Keywords: 45S5 bioactive glass, 3D bioprinting, bone tissue engineering, collagen gel, rheological properties, mesenchymal stem cells, alkaline phosphatase activity, calcium deposition
MeSH terms: Bone and Bones, Ceramics, Collagen, Humans, Ink, Tissue Engineering, Tissue Scaffolds, Mesenchymal Stem Cells, Printing, Three-Dimensional

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Bioglass incorporated methacrylated collagen bioactive ink for 3D printing of bone tissue

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