Cited by 7Algal biology and biofuel productionMicrobial Metabolic Engineering and BioproductionLipid metabolism and biosynthesis

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Enhancement of lipid production of Chlorella sp. 042 by mutagenesis

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Rahman, D Y, Rachmayati, R, Widyaningrum, D N, Susilaningsih, D·2020·IOP Conference Series Earth and Environmental Science

Abstract

Abstract In this study, the UV mutagenesis was performed to enhance lipid productivity in Chlorella sp. 042 isolated from East Kalimantan. One hundred colonies were screened with a method based on Nile Red fluorescence. Growth, biomass, and lipid parameters of the selected mutants (M7 and M22) were grown in a batch system of 400 mL AF6 medium and analyzed in detail. The result showed that the lipid content of M7 and M22 were higher than wild type (30.82%), 35.15%, and 43.85%, respectively. The M22 slightly had higher carbohydrate and protein content than wild type and M7. The overall result indicates that the treatment of UV mutagenesis to Chlorella sp. 042 can increase the total lipid production and other biomass content. The mutant could consecutively be used as the lipid feedstock for algal oil production.

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Crossref · 10.1088/1755-1315/439/1/012021

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Research works printed but not linked: 2

  1. Deng, 2009, Afican J. Microbial. Res., 3, 1008
  2. NIES-Collection List of Strains, Jpn. J. Phycol. (Sôrui), 57, 1

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Metadata

Authors
Rahman, D. Y., Rachmayati, R., Widyaningrum, D. N., & Susilaningsih, D.
Affiliations
Indonesian Institute of Sciences (ID)
Venue
IOP Conference Series Earth and Environmental Science
Published
2020-02-01
Volume
439
Issue
1
Pages
012021
ISSN
  • 1755-1315 (ISSN-L)
  • 1755-1307
OpenAlex
W3007885416
Cited by
7
References
17
Citations by year
2024: 1 · 2022: 2 · 2021: 2 · 2020: 2
FWCI
2.87
Type
conference paper
Language
en
Open access
Locations
IOP Conference Series Earth and Environmental Science (publishedVersion, cc-by)
Funders
Lembaga Ilmu Pengetahuan Indonesia
Topics
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Keywords
microalgal lipid production, UV mutagenesis, mutant selection, Nile red fluorescence, algal biomass composition, algal oil, biofuel feedstock

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