Optimization of an Analytical Procedure for Extraction of Lipids from Microalgae
Abstract
An optimized procedure for extraction of total and non‐polar lipids from microalgae is proposed. The effects of solvent, pretreatment (lyophilization, inactivation of lipases, and addition of antioxidants) and cell‐disruption (liquid nitrogen, sonication, and bead beating) on total lipid content, lipid class, and fatty acid composition were examined. Chloroform–methanol 1:1 was shown to be the best solvent mixture for extraction of total lipids from microalgae. When performing this extraction, lyophilized algae can be used, no pretreatment with isopropanol to inactivate the lipases is needed and addition of antioxidants is not necessary. Furthermore, cell‐disruption is not essential, although in that case two extractions must be performed in series to ensure that, irrespective of the microalgal species, all lipids are extracted. Determination of non‐polar lipid content should be performed by separation of the total lipid extract on an SPE column. Extraction using petroleum ether is only appropriate when a bead beater is used for pretreatment.
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Modification of solvent polarity (e.g., mixing hexane with ethanol or isopropanol) is necessary for high lipid recovery.
“In many cases, modification of solvent polarity (for example employing a mix of hexane with ethanol or isopropanol) is necessary for high lipid recovery (35-39).”
From Catalytic Hydroprocessing of Single-Cell Oils to Hydrocarbon Fuels: Converting microbial lipids to fuels is a promising approach to replace fossil fuels (2021) · cites this paper as [35]
Cites
A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189 · see citing works
A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Bligh, E. G., & Dyer, W. J. (1959) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/o59-099 · Cited by 54,751 · see citing works
A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Bligh, E. G., & Dyer, W. J. (1959) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/y59-099 · Cited by 23,189 · see citing works
Lipid extraction by methyl-tert-butyl ether for high-throughput lipidomics
Matyash, V., Liebisch, G., Kurzchalia, T. V., Shevchenko, A., & Schwudke, D. (2008) · Journal of Lipid Research · doi:10.1194/jlr.d700041-jlr200 · Cited by 2,615 · see citing works
Comparison of several methods for effective lipid extraction from microalgae
Lee, J.-Y., Yoo, C., Jun, S.-Y., Ahn, C.-Y., & Oh, H.-M. (2010) · Bioresource Technology · doi:10.1016/j.biortech.2009.03.058 · Cited by 1,279 · see citing works
YELLOW‐GREEN ALGAE WITH CHLOROPHYLLIDE C1,2
Guillard, R. R. L., & Lorenzen, C. J. (1972) · Journal of Phycology · doi:10.1111/j.1529-8817.1972.tb03995.x · Cited by 1,160 · see citing works
Lipids and lipid metabolism in eukaryotic algae
Guschina, I. A., & Harwood, J. L. (2006) · Progress in Lipid Research · doi:10.1016/j.plipres.2006.01.001 · Cited by 1,110 · see citing works
Form and function of phospholipids
ANSELL, G. B., Hawthorne, J. N., & DAWSON, R. M. C. (1973) · Medical Entomology and Zoology · Cited by 1,069 · see citing works
LI, H., CHENG, K., WONG, C., FAN, K., CHEN, F., & JIANG, Y. (2007) · Food Chemistry · doi:10.1016/j.foodchem.2006.06.022 · Cited by 790 · see citing works
Lipid analysis;: Isolation, separation, identification, and structural analysis of lipids
Christie, W. W. (1973) · Medical Entomology and Zoology · Cited by 755 · see citing works
Iverson, S. J., Lang, S. L. C., & Cooper, M. H. (2001) · Lipids · doi:10.1007/s11745-001-0843-0 · Cited by 719 · see citing works
Characterisation of algogenic organic matter extracted from cyanobacteria, green algae and diatoms
Henderson, R. K., Baker, A., Parsons, S. A., & Jefferson, B. (2007) · Water Research · doi:10.1016/j.watres.2007.10.032 · Cited by 707 · see citing works
Lipid Analysis: Isolation, Separation, Identification and Structural Analysis of Lipids
Berry, S. E. (2004) · Nutrition Bulletin · doi:10.1111/j.1467-3010.2003.00361.x · Cited by 471 · see citing works
Rapid method for the determination of lipid from the green alga Botryococcus braunii
Lee, S. J., Yoon, B.-D., & Oh, H.-M. (1998) · Biotechnology Techniques · doi:10.1023/a:1008811716448 · Cited by 368 · see citing works
A Simple and Rapid Solvent ExtractionMethod for Determining Total Lipids in Fish Tissue
Lee, C. M., Trevino, B., & Chaiyawat, M. (1996) · Journal of AOAC International · doi:10.1093/jaoac/79.2.487 · Cited by 337 · see citing works
SUGAR COMPOSITION OF THE CELL WALL AND THE TAXONOMY OF CHLORELLA (CHLOROPHYCEAE)1
Takeda, H. (1991) · Journal of Phycology · doi:10.1111/j.0022-3646.1991.00224.x · Cited by 179 · see citing works
Lipid analysis; A practical approach
(1994) · General Pharmacology The Vascular System · doi:10.1016/0306-3623(94)90045-0 · Cited by 178 · see citing works
Handbook of food analytical chemistry: water, proteins, enzymes, lipids, and carbohydrates.
Wrolstad, R. E., Acree, T. E., Decker, E. A., Penner, M. H., Reid, D. S., Schwartz, S. J., Shoemaker, C. F., Smith, D. H., & Sporns, P. (2005) · Wiley eBooks · doi:10.1002/0471709085 · Cited by 176 · see citing works
Comparison between extraction of lipids and fatty acids from microalgal biomass
Grima, E. M., Medina, A. R., Giménez, A. G., Pérez, J. A. S., García-Camacho, F., & Sánchez, J. L. G. (1994) · Journal of the American Oil Chemists Society · doi:10.1007/bf02542261 · Cited by 175 · see citing works
THE POLYMORPHIC DIATOM PHAEODACTYLUM TRICORNUTUM: ULTRASTRUCTURE OF ITS MORPHOTYPES1,2
Borowitzka, M. A., & Volcani, B. E. (1978) · Journal of Phycology · doi:10.1111/j.1529-8817.1978.tb00625.x · Cited by 144 · see citing works
Kangani, C. O., Kelley, D. E., & DeLany, J. P. (2008) · Journal of Chromatography B · doi:10.1016/j.jchromb.2008.08.009 · Cited by 101 · see citing works
Chen, G., JIANG, Y., & Chen, F. (2007) · Food Chemistry · doi:10.1016/j.foodchem.2007.03.008 · Cited by 86 · see citing works
Cell wall sugars of some Scenedesmus species
Takeda, H. (1996) · Phytochemistry · doi:10.1016/0031-9422(95)00952-3 · Cited by 84 · see citing works
Guckert, J. B., Cooksey, K. E., & Jackson, L. L. (1988) · Journal of Microbiological Methods · doi:10.1016/0167-7012(88)90015-2 · Cited by 82 · see citing works
On the morphology and ultrastructure of the cell wall of Spirulina platensis
van Eykelenburg, C. (1977) · Antonie van Leeuwenhoek · doi:10.1007/bf00395664 · Cited by 81 · see citing works
Anaerobic biodegradation of lipids of the marine microalga Nannochloropsis salina
Grossi, V., Blokker, P., & Damsté, J. S. S. (2001) · Organic Geochemistry · doi:10.1016/s0146-6380(01)00040-7 · Cited by 68 · see citing works
Corre, G., Templier, J., Largeau, C., Rousseau, B., & Berkaloff, C. (1996) · Journal of Phycology · doi:10.1111/j.0022-3646.1996.00584.x · Cited by 57 · see citing works
Extraction of lipids and derivative formation
Hamilton, S., Hamilton, R., & Sewell, P. A. (1992) · doi:10.1093/oso/9780199630981.003.0002 · Cited by 55 · see citing works
Dependence of Lipase Activity on Water Content and Enzyme Concentration in Reverse Micelles
Han, D., Walde, P., & Luisi, P. L. (1990) · Biocatalysis · doi:10.3109/10242429008992087 · Cited by 54 · see citing works
A glucan from the cell wall of the cyanobacterium Spirulina platensis
van Eykelenburg, C. (1978) · Antonie van Leeuwenhoek · doi:10.1007/bf00394309 · Cited by 21 · see citing works
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- Authors
- Ryckebosch, E., Muylaert, K., & Foubert, I.
- Affiliations
- KU Leuven (BE)
- Corresponding author
- Ryckebosch, Eline
- Venue
- Journal of the American Oil Chemists' Society
- Publisher
- Wiley
- Published
- 2011-07-24
- Volume
- 89
- Issue
- 2
- Pages
- 189-198
- ISSN
- 0003-021X (ISSN-L)
- 1558-9331
- OpenAlex
- W1983299190
- Cited by
- 481
- References
- 30
- Citations by year
- 2026: 20 · 2025: 36 · 2024: 33 · 2023: 37 · 2022: 31 · 2021: 40 · 2020: 34 · 2019: 43 · 2018: 29 · 2017: 36 · 2016: 42 · 2015: 33 · 2014: 36 · 2013: 19 · 2012: 10
- FWCI
- 8.90
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of the American Oil Chemists Society (publishedVersion)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Keywords
- microalgae, lipid extraction, total lipids, non-polar lipids, chloroform-methanol extraction, cell disruption, freeze-drying, lipase inactivation, antioxidants, fatty acid composition, solid-phase extraction, sonication, bead beating, liquid nitrogen
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