Cited by 481Algal biology and biofuel productionLipid metabolism and biosynthesisSeaweed-derived Bioactive Compounds

Optimization of an Analytical Procedure for Extraction of Lipids from Microalgae

Ryckebosch, Eline, Muylaert, Koenraad, Foubert, Imogen·2012·Journal of the American Oil Chemists' Society

Abstract

An optimized procedure for extraction of total and non‐polar lipids from microalgae is proposed. The effects of solvent, pretreatment (lyophilization, inactivation of lipases, and addition of antioxidants) and cell‐disruption (liquid nitrogen, sonication, and bead beating) on total lipid content, lipid class, and fatty acid composition were examined. Chloroform–methanol 1:1 was shown to be the best solvent mixture for extraction of total lipids from microalgae. When performing this extraction, lyophilized algae can be used, no pretreatment with isopropanol to inactivate the lipases is needed and addition of antioxidants is not necessary. Furthermore, cell‐disruption is not essential, although in that case two extractions must be performed in series to ensure that, irrespective of the microalgal species, all lipids are extracted. Determination of non‐polar lipid content should be performed by separation of the total lipid extract on an SPE column. Extraction using petroleum ether is only appropriate when a bead beater is used for pretreatment.

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Crossref · 10.1007/s11746-011-1903-z

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Authors
Ryckebosch, E., Muylaert, K., & Foubert, I.
Affiliations
KU Leuven (BE)
Corresponding author
Ryckebosch, Eline
Venue
Journal of the American Oil Chemists' Society
Publisher
Wiley
Published
2011-07-24
Volume
89
Issue
2
Pages
189-198
ISSN
  • 0003-021X (ISSN-L)
  • 1558-9331
OpenAlex
W1983299190
Cited by
481
References
30
Citations by year
2026: 20 · 2025: 36 · 2024: 33 · 2023: 37 · 2022: 31 · 2021: 40 · 2020: 34 · 2019: 43 · 2018: 29 · 2017: 36 · 2016: 42 · 2015: 33 · 2014: 36 · 2013: 19 · 2012: 10
FWCI
8.90
Type
journal article
Language
en
Open access
closed
Locations
Journal of the American Oil Chemists Society (publishedVersion)
Topics
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
Keywords
microalgae, lipid extraction, total lipids, non-polar lipids, chloroform-methanol extraction, cell disruption, freeze-drying, lipase inactivation, antioxidants, fatty acid composition, solid-phase extraction, sonication, bead beating, liquid nitrogen

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