The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Atlantic Salmon Interferon Genes: Cloning, Sequence Analysis, Expression, and Biological Activity
Abstract
In this work, we report cDNA cloning of two type I interferons (IFNs) from the head kidney of Atlantic salmon, called SasaIFN-alpha1 (829 bp) and SasaIFN-alpha2 (1290 bp). Both translate into 175 amino acid precursor molecules showing 95% amino acid sequence identity. The precursors have a putative 23 amino acid signal peptide, which suggests that the mature Atlantic salmon IFNs contain 152 amino acids (18.2 kDa). Salmon IFN appears to have five alpha-helices, similar to mammalian and avian type I IFNs, and showed 45% sequence identity with zebrafish IFN, up to 29% identity with mammalian IFN-alpha sequences, and 17%-18% sequence identity with mammalian IFN-beta and chicken type I IFNs. Human embryonic kidney 293 cells transfected with the SasaIFN-alpha1 cDNA gene produced high titers of acid-stable antiviral activity, which protected salmonid cells against infectious pancreatic necrosis virus (IPNV) and also induced Mx protein in the cells. Poly(I)-poly(C) induced two IFN transcripts in head kidney of Atlantic salmon. Genomic IFN sequences contained four introns and five exons, which is different from the intronless type I IFN genes of birds and mammals.
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Cites
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Cited by
Interferons, interferon‐like cytokines, and their receptors
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The interferon system of teleost fish
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Stein, C., Caccamo, M., Laird, G., & Leptin, M. (2007) · Genome Biology · doi:10.1186/gb-2007-8-11-r251 · Cited by 374
Smith, N. C., Rise, M. L., & Christian, S. L. (2019) · Frontiers in Immunology · doi:10.3389/fimmu.2019.02292 · Cited by 304
Metadata
- Authors
- Robertsen, B., Bergan, V., Røkenes, T., Larsen, R., & Albuquerque, A.
- Venue
- Journal of Interferon & Cytokine Research
- Publisher
- SAGE Publications
- Published
- 2003-10
- Volume
- 23
- Issue
- 10
- Pages
- 601-612
- ISSN
- 1079-9907 (ISSN-L)
- 1557-7465
- OpenAlex
- W2053164681
- Cited by
- 261
- References
- 34
- FWCI
- 4.16
- Type
- journal article
- Language
- en
- Open access
- closed
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