The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Zebrafish: A Preclinical Model for Drug Screening
Abstract
The zebrafish embryo has become an important vertebrate model for assessing drug effects. It is well suited for studies in genetics, embryology, development, and cell biology. Zebrafish embryos exhibit unique characteristics, including ease of maintenance and drug administration, short reproductive cycle, and transparency that permits visual assessment of developing cells and organs. Because of these advantages, zebrafish bioassays are cheaper and faster than mouse assays, and are suitable for large-scale drug screening. Here we describe the use of zebrafish bioassays for assessing toxicity, angiogenesis, and apoptosis. Using 18 chemicals, we demonstrated that toxic response, teratogenic effects, and LC(50) in zebrafish are comparable to results in mice. The effects of compounds on various organs, including the heart, brain, intestine, pancreas, cartilage, liver, and kidney, were observed in the transparent animals without complicated processing, demonstrating the efficiency of toxicity assays using zebrafish embryos. Using endogenous alkaline phosphatase staining and a whole-animal enzyme assay, we demonstrated that SU5416 and flavopiridol, compounds shown to have antiangiogenic effects in mammals, inhibit blood vessel growth in zebrafish, and this bioassay is suitable for high-throughput screening using a 96-well microplate reader. We also demonstrated that in vivo acridine orange staining can be used to visualize apoptotic events in embryos treated with brefeldin A, neomycin, or caspase inhibitors. After in vivo staining, acridine orange can be extracted and quantitated using a fluorescence microplate reader, providing a screening system for agents that modulate apoptosis.
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The Zebrafish Book : A Guide for the Laboratory Use of Zebrafish
Westerfield, M. (2000) · Medical Entomology and Zoology · Cited by 772
Zebrafish: a model system for the study of human disease
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Morpholino antisense oligomers: the case for an RNase H-independent structural type
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Small molecule developmental screens reveal the logic and timing of vertebrate development
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Mutations affecting the cardiovascular system and other internal organs in zebrafish
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Zebrafish tinman homolog demarcates the heart field and initiates myocardial differentiation
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Cited by
Zebrafish as a Model Vertebrate for Investigating Chemical Toxicity
Hill, A. J., Teraoka, H., Heideman, W., & Peterson, R. E. (2005) · Toxicological Sciences · doi:10.1093/toxsci/kfi110 · Cited by 1,374
Scholz, S., Fischer, S., Gündel, U., Küster, E., Luckenbach, T., & Voelker, D. (2008) · Environmental Science and Pollution Research · doi:10.1007/s11356-008-0018-z · Cited by 593
Zebrafish: a predictive model for assessing drug-induced toxicity
McGrath, P., & Li, C.-Q. (2008) · Drug Discovery Today · doi:10.1016/j.drudis.2008.03.002 · Cited by 521
Self-citationZebrafish embryos and larvae: A new generation of disease models and drug screens
Ali, S., Champagne, D. L., Spaink, H. P., & Richardson, M. K. (2011) · Birth Defects Research Part C: Embryo Today: Reviews · doi:10.1002/bdrc.20206 · Cited by 257
Singulani, J. D. L., Scorzoni, L., Gomes, P. C., Nazaré, A. C., Polaquini, C. R., Regasini, L. O., Fusco-Almeida, A. M., & Mendes-Giannini, M. J. S. (2017) · Future Medicinal Chemistry · doi:10.4155/fmc-2017-0096 · Cited by 33
Metadata
- Authors
- Parng, C., Seng, W. L., Semino, C., & McGrath, P.
- Venue
- ASSAY and Drug Development Technologies
- Publisher
- SAGE Publications
- Published
- 2002-11
- Volume
- 1
- Issue
- 1
- Pages
- 41-48
- ISSN
- 1540-658X (ISSN-L)
- 1557-8127
- OpenAlex
- W2149657563
- Cited by
- 403
- References
- 25
- FWCI
- 1.14
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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