The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.
A Method for Isolation of Intact, Translationally Active Ribonucleic Acid
Abstract
A method for isolation of large, translationally active RNA species is presented. The procedure involves homogenization of cells or tissues in 5 M guanidine monothiocyanate followed by direct precipitation of RNA from the guanidinium by 4 M LiCl. Modifications are described for use with tissue culture cells, yeast, tissues, or isolated nuclei. The advantages of the procedure include speed, simplicity, avoidance of an ultracentrifugation, and its applicability to large numbers of small samples. The procedure yields large mRNA precursors up to 10 kb and mRNA species which translate very well. However, small (less than 300 nucleotides) RNA species are recovered with a poor yield.
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Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease
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PELHAM, H. R. B., & JACKSON, R. J. (1976) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1976.tb10656.x · Cited by 3,926
Purification of Mouse Immunoglobulin Heavy‐Chain Messenger RNAs from Total Myeloma Tumor RNA
AUFFRAY, C., & ROUGEON, F. (1980) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1980.tb06030.x · Cited by 2,627
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Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormone
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Self-citationA restriction map of the bacteriophage T4 genome
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Cited by
A protein related to extracellular matrix proteins deleted in the mouse mutant reeler
D'Arcangelo, G., G. Miao, G., Chen, S.-C., Scares, H. D., Morgan, J. I., & Curran, T. (1995) · Nature · doi:10.1038/374719a0 · Cited by 1,748
Charge density-dependent strength of hydration and biological structure
Collins, K. D. (1997) · Biophysical Journal · doi:10.1016/s0006-3495(97)78647-8 · Cited by 1,334
Kawasaki, E. S., Clark, S. S., Coyne, M. Y., Smith, S. D., Champlin, R., Witte, O. N., & McCormick, F. P. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.15.5698 · Cited by 709
Guanylyl cyclase is a heat-stable enterotoxin receptor
Schulz, S., Green, C. K., Yuen, P. S. T., & Garbers, D. L. (1990) · Cell · doi:10.1016/0092-8674(90)90497-3 · Cited by 601
Kavanaugh, M. P., Miller, D. G., Zhang, W., Law, W., Kozak, S. L., Kabat, D., & Miller, A. D. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.15.7071 · Cited by 586
Metadata
- Authors
- CATHALA, G., SAVOURET, J.-F., MENDEZ, B., WEST, B. L., KARIN, M., MARTIAL, J. A., & BAXTER, J. D.
- Venue
- DNA
- Publisher
- SAGE Publications
- Published
- 1983-12
- Volume
- 2
- Issue
- 4
- Pages
- 329-335
- ISSN
- 0198-0238 (ISSN-L)
- OpenAlex
- W2088986997
- Cited by
- 1,447
- References
- 11
- FWCI
- 12.11
- Type
- journal article
- Language
- en
- Open access
- closed
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- CATHALA, G., SAVOURET, J.-F., MENDEZ, B., WEST, B. L., KARIN, M., MARTIAL, J. A., & BAXTER, J. D. (1983). A Method for Isolation of Intact, Translationally Active Ribonucleic Acid. DNA. https://doi.org/10.1089/dna.1983.2.329
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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