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Preadipocyte Seeded PLGA Scaffolds for Adipose Tissue Engineering
Abstract
Adipose tissue equivalents have not been addressed as yet despite the clinical need in congenital deformities, posttraumatic repair, cancer rehabilitation, and other soft tissue defects. Preadipocytes were successfully harvested from rat epididymal fat pads of Sprague-Dawley and Lewis rats and expanded ex vivo. In vitro cultures demonstrated full differentiation of preadipocytes into mature adipocytes with normal lipogenic activity. The onset of differentiation was well-controlled by regulating preadipocyte confluency. Poly(lactic-co-glycolic) acid (PLGA) polymer disks with 90% porosity, 2.5 mm thick, 12 mm diameter, pore size range of 135-633 microm were fabricated and seeded with preadipocytes at 10(5) cells/mL. Disks in vitro demonstrated fully differentiated mature adipocytes within the pores of the disks. Short-term in vivo experiments were conducted by implanting preseeded disks subcutaneously on the flanks of rats for 2 and 5 weeks. Histologic staining of harvested disks with osmium tetroxide (OsO4) revealed the formation of adipose tissue throughout the disks. Fluorescence labeling of preadipocytes confirmed that formed adipose tissue originated from seeded preadipocytes rather than from possible infiltrating perivascular tissue. This study demonstrates the potential of using primary preadipocytes as a cell source in cell-seeded polymer scaffolds for tissue engineering applications.
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Cited by
Comparison of Multi-Lineage Cells from Human Adipose Tissue and Bone Marrow
De Ugarte, D. A., Morizono, K., Elbarbary, A., Alfonso, Z., Zuk, P. A., Zhu, M., Dragoo, J. L., Ashjian, P., Thomas, B., Benhaim, P., et al. (2003) · Cells Tissues Organs · doi:10.1159/000071150 · Cited by 1,173
How smart do biomaterials need to be? A translational science and clinical point of view
Holzapfel, B. M., Reichert, J. C., Schantz, J.-T., Gbureck, U., Rackwitz, L., Nöth, U., Jakob, F., Rudert, M., Groll, J., & Hutmacher, D. W. (2013) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2012.07.009 · Cited by 552
Unger, R. E., Sartoris, A., Peters, K., Motta, A., Migliaresi, C., Kunkel, M., Bulnheim, U., Rychly, J., & James Kirkpatrick, C. (2007) · Biomaterials · doi:10.1016/j.biomaterials.2007.05.032 · Cited by 385
Mauney, J. R., Nguyen, T., Gillen, K., Kirker-Head, C., Gimble, J. M., & Kaplan, D. L. (2007) · Biomaterials · doi:10.1016/j.biomaterials.2007.08.017 · Cited by 373
Adipose-Derived Stem and Progenitor Cells as Fillers in Plastic and Reconstructive Surgery
Moseley, T. A., Zhu, M., & Hedrick, M. H. (2006) · Plastic & Reconstructive Surgery · doi:10.1097/01.prs.0000234609.74811.2e · Cited by 335
Metadata
- Authors
- Patrick, C. W., Chauvin, P. B., Hobley, J., & Reece, G. P.
- Venue
- Tissue Engineering
- Publisher
- SAGE Publications
- Published
- 1999-04
- Volume
- 5
- Issue
- 2
- Pages
- 139-151
- ISSN
- 1076-3279 (ISSN-L)
- 1557-8690
- OpenAlex
- W2052846669
- Cited by
- 283
- References
- 0
- FWCI
- 10.06
- Type
- journal article
- Language
- en
- Open access
- closed
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- Patrick, C. W., Chauvin, P. B., Hobley, J., & Reece, G. P. (1999). Preadipocyte Seeded PLGA Scaffolds for Adipose Tissue Engineering. Tissue Engineering. https://doi.org/10.1089/ten.1999.5.139
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