Engineering adipose-like tissue in vitro and in vivo utilizing human bone marrow and adipose-derived mesenchymal stem cells with silk fibroin 3D scaffolds
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Biomaterials derived from silk fibroin prepared by aqueous (AB) and organic (HFIP) solvent-based processes, along with collagen (COL) and poly-lactic acid (PLA)-based scaffolds were studied in vitro and in vivo for their utility in adipose tissue engineering strategies. For in vitro studies, human bone marrow and adipose-derived mesenchymal stem cells (hMSCs and hASCs) were seeded on the various biomaterials and cultured for 21 days in the presence of adipogenic stimulants (AD) or maintained as noninduced controls. Alamar Blue analysis revealed each biomaterial supported initial attachment of hMSCs and hASCs to similar levels for all matrices except COL in which higher levels were observed. hASCs and hMSCs cultured on all biomaterials in the presence of AD showed significant upregulation of adipogenic mRNA transcript levels (LPL, GLUT4, FABP4, PPARgamma, adipsin, ACS) to similar extents when compared to noninduced controls. Similarly Oil-Red O analysis of hASC or hMSC-seeded scaffolds displayed substantial amounts of lipid accumulating adipocytes following cultivation with AD. The data revealed AB and HFIP scaffolds supported similar extents of lipid accumulating cells while PLA and COL scaffolds qualitatively displayed lower and higher extents by comparison, respectively. Following a 4-week implantation period in a rat muscle pouch defect model, both AB and HFIP scaffolds supported in vivo adipogenesis either alone or seeded with hASCs or hMSCs as assessed by Oil-Red O analysis, however the presence of exogenous cell sources substantially increased the extent and frequency of adipogenesis observed. In contrast, COL and PLA scaffolds underwent rapid scaffold degradation and were irretrievable following the implantation period. The results suggest that macroporous 3D AB and HFIP silk fibroin scaffolds offer an important platform for cell-based adipose tissue engineering applications, and in particular, provide longer-term structural integrity to promote the maintenance of soft tissue in vivo.
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Silk fibroin-based scaffold does not contain cell-specific signal expression in its primary sequence but provides a suitable matrix for supporting the proliferation and adhesion of mesenchymal stem cells.
“Although the silk protein-based scaffold does not contain any cell-specific signal expression in its primary sequence, it provides a suitable matrix for supporting the proliferation and adhesion of mesenchymal stem cells [31,32].”
From Recent Advances in Silk Fibroin-Based Composites for Bone Repair Applications: A Review (2025) · cites this paper as [32]
The Results (page not available in our copy) show that human mesenchymal stem cells adhered to silk scaffolds, supporting the adhesion and suitable-matrix portion of the claim. The paper does not establish that the scaffolds support stem-cell proliferation or state that silk fibroin lacks cell-specific signals in its primary sequence. (LLM, conf=0.88)
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Cites
Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1006/abio.1987.9999 · Cited by 65,240 · see citing works
Altman, G. H., Diaz, F., Jakuba, C., Calabro, T., Horan, R. L., Chen, J., Lu, H., Richmond, J., & Kaplan, D. L. (2003) · Biomaterials · doi:10.1016/s0142-9612(02)00353-8 · Cited by 3,458 · see citing works
Self-citationPPARγ Is Required for the Differentiation of Adipose Tissue In Vivo and In Vitro
Rosen, E. D., Sarraf, P., Troy, A. E., Bradwin, G., Moore, K. J., Milstone, D. S., Spiegelman, B. M., & Mortensen, R. M. (1999) · Molecular Cell · doi:10.1016/s1097-2765(00)80211-7 · Cited by 2,081 · see citing works
Principles of Tissue Engineering
(2014) · Elsevier eBooks · doi:10.1016/c2011-0-07193-4 · Cited by 1,909 · see citing works
C/EBPα induces adipogenesis through PPARγ: a unified pathwayPreprint
Rosen, E. D., Hsu, C.-H., Wang, X., Sakai, S., Freeman, M. W., Gonzalez, F. J., & Spiegelman, B. M. (2002) · Genes & Development · doi:10.1101/gad.948702 · Cited by 1,497 · see citing works
Control of the peroxisomal β-oxidation pathway by a novel family of nuclear hormone receptors
Dreyer, C., Krey, G. D., Keller, H., Givel, F., Helftenbein, G., & Wahli, W. (1992) · Cell · doi:10.1016/0092-8674(92)90031-7 · Cited by 1,373 · see citing works
PPARγ Mediates High-Fat Diet–Induced Adipocyte Hypertrophy and Insulin Resistance
Kubota, N., Terauchi, Y., Miki, H., Tamemoto, H., Yamauchi, T., Komeda, K., Satoh, S., Nakano, R., Ishii, C., 杉山, 拓., et al. (1999) · Molecular Cell · doi:10.1016/s1097-2765(00)80210-5 · Cited by 1,342 · see citing works
Schoonjans, K., Staels, B., & Auwerx, J. H. (1996) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(96)00066-5 · Cited by 1,047 · see citing works
Three-dimensional aqueous-derived biomaterial scaffolds from silk fibroin
Kim, U., Park, J. H., Kim, H. J., Wada, M., & Kaplan, D. L. (2004) · Biomaterials · doi:10.1016/j.biomaterials.2004.07.044 · Cited by 979 · see citing works
Self-citationPorous 3-D Scaffolds from Regenerated Silk Fibroin
Nazarov, R., Jin, H., & Kaplan, D. L. (2004) · Biomacromolecules · doi:10.1021/bm034327e · Cited by 888 · see citing works
Self-citationThe inflammatory responses to silk films in vitro and in vivo
Meinel, L., Hofmann, S., Karageorgiou, V., Kirker‐Head, C. A., McCool, J., Gronowicz, G., Zichner, L., Langer, R., Vunjak‐Novakovic, G., & Kaplan, D. L. (2004) · Biomaterials · doi:10.1016/j.biomaterials.2004.02.047 · Cited by 785 · see citing works
Self-citationIn vitro degradation of silk fibroin
Horan, R. L., Antle, K. A., Collette, A. L., Wang, Y., Huang, J., Moreau, J. E., Volloch, V., Kaplan, D. L., & Altman, G. H. (2004) · Biomaterials · doi:10.1016/j.biomaterials.2004.09.020 · Cited by 741 · see citing works
Self-citationElectrospinning Bombyx mori Silk with Poly(ethylene oxide)
Jin, H., Fridrikh, S. V., Rutledge, G. C., & Kaplan, D. L. (2002) · Biomacromolecules · doi:10.1021/bm025581u · Cited by 715 · see citing works
Self-citationCell differentiation by mechanical stress
Altman, G. H., Horan, R. L., Martín, I., Farhadi, J., Stark, P. R. H., Volloch, V., Richmond, J. C., Vunjak‐Novakovic, G., & Kaplan, D. L. (2001) · The FASEB Journal · doi:10.1096/fj.01-0656fje · Cited by 670 · see citing works
Self-citationPPARγ, the ultimate thrifty gene
Auwerx, J. (1999) · Diabetologia · doi:10.1007/s001250051268 · Cited by 612 · see citing works
Principles of Tissue Engineering
Pegg, D. E. (1999) · Cryobiology · doi:10.1006/cryo.1999.2214 · Cited by 566 · see citing works
Meinel, L., Karageorgiou, V., Fajardo, R., Snyder, B. D., Patil, V. R. S., Zichner, L., Kaplan, D. L., Langer, R., & Vunjak‐Novakovic, G. (2003) · Annals of Biomedical Engineering · doi:10.1023/b:abme.0000007796.48329.b4 · Cited by 561 · see citing works
Self-citationSignaling Mechanisms That Regulate Glucose Transport
Czech, M., & Corvera, S. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.4.1865 · Cited by 514 · see citing works
The Adipocyte as an Endocrine Cell
Halberg, N., Asterholm, I. W., & Scherer, P. E. (2008) · Endocrinology and Metabolism Clinics of North America · doi:10.1016/j.ecl.2008.07.002 · Cited by 442 · see citing works
Cartilage tissue engineering with silk scaffolds and human articular chondrocytes
Wang, Y., Blasioli, D. J., Kim, H.-J., Kim, H. S., & Kaplan, D. L. (2006) · Biomaterials · doi:10.1016/j.biomaterials.2006.03.050 · Cited by 420 · see citing works
Self-citationHuman adipsin is identical to complement factor D and is expressed at high levels in adipose tissue.
White, R. T., Damm, D. L., Hancock, N., Rosen, B. S., Lowell, B. B., Usher, P., Flier, J. S., & Spiegelman, B. M. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)50409-4 · Cited by 409 · see citing works
Frank, O., Heim, M., Jakob, M., Barbero, A., Schäfer, D., Bendik, I., Dick, W., Heberer, M., & Martín, I. (2002) · Journal of Cellular Biochemistry · doi:10.1002/jcb.10174 · Cited by 370 · see citing works
Engineering bone‐like tissue in vitro using human bone marrow stem cells and silk scaffolds
Meinel, L., Karageorgiou, V., Hofmann, S., Fajardo, R., Snyder, B. D., Li, C., Zichner, L., Langer, R., Vunjak‐Novakovic, G., & Kaplan, D. L. (2004) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.30117 · Cited by 349 · see citing works
Self-citationEngineering cartilage‐like tissue using human mesenchymal stem cells and silk protein scaffolds
Meinel, L., Hofmann, S., Karageorgiou, V., Zichner, L., Langer, R., Kaplan, D. L., & Vunjak‐Novakovic, G. (2004) · Biotechnology and Bioengineering · doi:10.1002/bit.20252 · Cited by 310 · see citing works
Self-citationFatty Acid Binding Proteins—The Evolutionary Crossroads of Inflammatory and Metabolic Responses
Makowski, L., & Hotamışlıgil, G. S. (2004) · Journal of Nutrition · doi:10.1093/jn/134.9.2464s · Cited by 308 · see citing works
Preadipocyte Seeded PLGA Scaffolds for Adipose Tissue Engineering
Patrick, C., Chauvin, P., Hobley, J., & Reece, G. P. (1999) · Tissue Engineering · doi:10.1089/ten.1999.5.139 · Cited by 283 · see citing works
Molecular regulation of adipocyte differentiation
Cowherd, R. M., Lyle, R., & McGehee, R. E. (1999) · Seminars in Cell and Developmental Biology · doi:10.1006/scdb.1998.0276 · Cited by 271 · see citing works
Frontiers in Tissue Engineering
(1998) · Elsevier eBooks · doi:10.1016/b978-0-08-042689-1.x5001-8 · Cited by 267 · see citing works
Kimura, Y., Ozeki, M., Inamoto, T., & Tabata, Y. (2003) · Biomaterials · doi:10.1016/s0142-9612(03)00049-8 · Cited by 257 · see citing works
Tissue engineering strategies for adipose tissue repair
Patrick, C. (2001) · The Anatomical Record · doi:10.1002/ar.1113 · Cited by 255 · see citing works
Human preadipocytes seeded on freeze-dried collagen scaffolds investigated in vitro and in vivo
von Heimburg, D., Zachariah, S., Kühling, H., Heschel, I., Schoof, H., Hafemann, B., & Pallua, N. (2001) · Biomaterials · doi:10.1016/s0142-9612(00)00186-1 · Cited by 229 · see citing works
Long-Term Implantation of Preadipocyte-Seeded PLGA Scaffolds
Patrick, C., Zheng, B., Johnston, C. M., & Reece, G. P. (2002) · Tissue Engineering · doi:10.1089/107632702753725049 · Cited by 210 · see citing works
Mauney, J. R., Jaquiéry, C., Volloch, V., Heberer, M., Martín, I., & Kaplan, D. L. (2004) · Biomaterials · doi:10.1016/j.biomaterials.2004.08.020 · Cited by 183 · see citing works
Self-citationApplication and evaluation of the alamarblue assay for cell growth and survival of fibroblasts
Voytik‐Harbin, S. L., Brightman, A. O., Waisner, B. Z., Lamar, C. H., & Badylak, S. F. (1998) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1007/s11626-998-0130-x · Cited by 167 · see citing works
Samuelsson, L., Strömberg, K., Vikman, K., Bjursell, G., & Enerbäck, S. (1991) · The EMBO Journal · doi:10.1002/j.1460-2075.1991.tb04948.x · Cited by 160 · see citing works
Lazar, M. A. (2004) · Biochimie · doi:10.1016/j.biochi.2004.10.021 · Cited by 138 · see citing works
Engineering of volume-stable adipose tissues
Cho, S., Kim, S. S., Rhie, J. W., Cho, H. M., Choi, C. Y., & Kim, B. (2004) · Biomaterials · doi:10.1016/j.biomaterials.2004.09.013 · Cited by 135 · see citing works
The adipocyte as an endocrine cell1
Miner, J. L. (2004) · Journal of Animal Science · doi:10.2527/2004.823935x · Cited by 131 · see citing works
Mauney, J. R., Volloch, V., & Kaplan, D. L. (2005) · Biomaterials · doi:10.1016/j.biomaterials.2005.03.024 · Cited by 126 · see citing works
Self-citationBioreactor cultivation of osteochondral grafts
Vunjak‐Novakovic, G., Meinel, L., Altman, G. H., & Kaplan, D. L. (2005) · Orthodontics and Craniofacial Research · doi:10.1111/j.1601-6343.2005.00334.x · Cited by 120 · see citing works
Self-citationNeubauer, M., Hacker, M. C., Bauer‐Kreisel, P., Weiser, B., Fischbach, C., Schulz‐Siegmund, M., Göpferich, A. M., & Blunk, T. (2005) · Tissue Engineering · doi:10.1089/ten.2005.11.1840 · Cited by 119 · see citing works
Fischbach, C., Spruß, T., Weiser, B., Neubauer, M., Becker, C., Hacker, M. C., Göpferich, A. M., & Blunk, T. (2004) · Experimental Cell Research · doi:10.1016/j.yexcr.2004.05.036 · Cited by 115 · see citing works
Adipogenic differentiation of adipose tissue derived adult stem cells in nude mouse
Choi, Y. S., Cha, S. M., Lee, Y. Y., Kwon, S. W., Park, C. J., & Kim, M. (2006) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2006.04.128 · Cited by 104 · see citing works
Engineered Adipose Tissue Supplied by Functional Microvessels
Borges, J., Mueller, M. C., Padron, N. T., Tegtmeier, F. T., Lang, E., & Stark, G. B. (2003) · Tissue Engineering · doi:10.1089/10763270360728170 · Cited by 88 · see citing works
Kang, X., Xie, Y., & Kniss, D. A. (2005) · Tissue Engineering · doi:10.1089/ten.2005.11.458 · Cited by 80 · see citing works
Three-Dimensional in Vitro Model of Adipogenesis: Comparison of Culture Conditions
Fischbach, C., Seufert, J., Staiger, H., Hacker, M. C., Neubauer, M., Göpferich, A. M., & Blunk, T. (2004) · Tissue Engineering · doi:10.1089/107632704322791862 · Cited by 71 · see citing works
De novo adipose formation in a vascularized engineered construct
Walton, R. L., Beahm, E. K., & Wu, L. C. (2004) · Microsurgery · doi:10.1002/micr.20056 · Cited by 70 · see citing works
Induction of preadipocyte differentiation by mature fat cells in the rat.
Shillabeer, G., Forden, J., & Lau, D. C. W. (1989) · Journal of Clinical Investigation · doi:10.1172/jci114177 · Cited by 62 · see citing works
Expression and regulation of pOb24 and lipoprotein lipase genes during adipose conversion
Dani, C., Amri, E., Bertrand, B., Enerbäck, S., Bjursell, G., Grimaldi, P. A., & Ailhaud, G. P. (1990) · Journal of Cellular Biochemistry · doi:10.1002/jcb.240430202 · Cited by 56 · see citing works
Fatty acid binding proteins stabilize leukotriene A4
Zimmer, J. S. D., Dyckes, D. F., Bernlohr, D., & Murphy, R. C. (2004) · Journal of Lipid Research · doi:10.1194/jlr.m400240-jlr200 · Cited by 55 · see citing works
Lipografting: Autologous Fat Grafting for Total Facial Rejuvenation
Tzikas, T. L. (2004) · Facial Plastic Surgery · doi:10.1055/s-2004-861754 · Cited by 50 · see citing works
Kim, C.-S., Kawada, T., Yoo, H., Kwon, B., & Yu, R. (2003) · FEBS Letters · doi:10.1016/s0014-5793(03)00728-2 · Cited by 45 · see citing works
Shimomura, I., Tokunaga, K., Jiao, S., Funahashi, T., Keno, Y., Kobatake, T., Kotani, K., Suzuki, H., Yamamoto, T. T., Tarui, S., et al. (1992) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(92)90086-b · Cited by 42 · see citing works
Diabetes with partial lipodystrophy following sclerodermatous chronic graft vs. host disease
Rooney, D. P., & Ryan, M. F. (2006) · Diabetic Medicine · doi:10.1111/j.1464-5491.2006.01855.x · Cited by 34 · see citing works
Lee, K. Y., Halberstadt, C. R., Holder, W. D., & Mooney, D. J. (2000) · Elsevier eBooks · doi:10.1016/b978-012436630-5/50035-0 · Cited by 26 · see citing works
Powers, M. P., & Bosker, H. (1996) · Journal of Oral and Maxillofacial Surgery · doi:10.1016/s0278-2391(96)90386-9 · Cited by 19 · see citing works
Osseous tissue engineering in oncologic surgery
Miller, M. J. (2000) · Seminars in Surgical Oncology · doi:10.1002/1098-2388(200010/11)19:3 · Cited by 9 · see citing works
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Sorted by citations
Materials fabrication from Bombyx mori silk fibroin
Rockwood, D. N., Preda, R. C., Yücel, T., Wang, X., Lovett, M. L., & Kaplan, D. L. (2011) · Nature Protocols · doi:10.1038/nprot.2011.379 · Cited by 3,096 · see citing works
Self-citationBiopolymer-Based Hydrogels As Scaffolds for Tissue Engineering Applications: A Review
Vlierberghe, S. V., Dubruel, P., & Schacht, E. H. (2011) · Biomacromolecules · doi:10.1021/bm200083n · Cited by 1,719 · see citing works
Biodegradation of Silk Biomaterials
Cao, Y., & Wang, B. (2009) · International Journal of Molecular Sciences · doi:10.3390/ijms10041514 · Cited by 719 · see citing works
Advances in 3D cell culture technologies enabling tissue‐like structures to be created in vitro
Knight, E., & Przyborski, S. A. (2014) · Journal of Anatomy · doi:10.1111/joa.12257 · Cited by 531 · see citing works
Biomedical applications of chemically-modified silk fibroin
Murphy, A. R., & Kaplan, D. L. (2009) · Journal of Materials Chemistry · doi:10.1039/b905802h · Cited by 522 · see citing works
Self-citationPolymeric materials based on silk proteins
Hardy, J. G., Römer, L., & Scheibel, T. (2008) · Polymer · doi:10.1016/j.polymer.2008.08.006 · Cited by 506 · see citing works
Digital light processing 3D printed silk fibroin hydrogel for cartilage tissue engineering
Hong, H., Seo, Y. B., Kim, D. Y., Lee, J. S., Lee, Y. J., Lee, H., Ajiteru, O., Sultan, M. T., Lee, O. J., Kim, S. H., et al. (2019) · Biomaterials · doi:10.1016/j.biomaterials.2019.119679 · Cited by 473 · see citing works
Silk proteins for biomedical applications: Bioengineering perspectives
Kundu, B., Kurland, N. E., Bano, S., Patra, C., Engel, F. B., Yadavalli, V. K., & Kundu, S. C. (2013) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2013.09.002 · Cited by 453 · see citing works
Adipose tissue stem cells meet preadipocyte commitment: going back to the future
Cawthorn, W. P., Scheller, E. L., & MacDougald, O. A. (2011) · Journal of Lipid Research · doi:10.1194/jlr.r021089 · Cited by 440 · see citing works
Silk fibroin/hydroxyapatite composites for bone tissue engineering
Farokhi, M., Mottaghitalab, F., Samani, S., Shokrgozar, M. A., Kundu, S. C., Reis, R. L., Fatahi, Y., & Kaplan, D. L. (2017) · Biotechnology Advances · doi:10.1016/j.biotechadv.2017.10.001 · Cited by 435 · see citing works
Self-citationHighly Tunable Elastomeric Silk Biomaterials
Partlow, B. P., Hanna, C. W., Rnjak‐Kovacina, J., Moreau, J. E., Applegate, M. B., Burke, K. A., Marelli, B., Mitropoulos, A. N., Omenetto, F. G., & Kaplan, D. L. (2014) · Advanced Functional Materials · doi:10.1002/adfm.201400526 · Cited by 430 · see citing works
Self-citationElectrospun silk biomaterial scaffolds for regenerative medicine
Zhang, X., Reagan, M. R., & Kaplan, D. L. (2009) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2009.07.005 · Cited by 432 · see citing works
Self-citationMathieu, P. S., & Loboa, E. G. (2012) · Tissue Engineering Part B Reviews · doi:10.1089/ten.teb.2012.0014 · Cited by 380 · see citing works
The Adipose-derived Stem Cell: Looking Back and Looking Ahead
Zuk, P. A. (2010) · Molecular Biology of the Cell · doi:10.1091/mbc.e09-07-0589 · Cited by 350 · see citing works
The use of skin models in drug development
Mathes, S. H., Ruffner, H., & Graf‐Hausner, U. (2014) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2013.12.006 · Cited by 321 · see citing works
Injectable Biomaterials for Regenerating Complex Craniofacial Tissues
Kretlow, J. D., Young, S. W., Klouda, L., Wong, M. E., & Mikos, A. G. (2009) · Advanced Materials · doi:10.1002/adma.200802009 · Cited by 302 · see citing works
Adipose‐Derived Stem Cells for Tissue Engineering and Regenerative Medicine Applications
Dai, R., Wang, Z., Samanipour, R., Koo, K.-I., & Kim, K. (2016) · Stem Cells International · doi:10.1155/2016/6737345 · Cited by 304 · see citing works
Poly(Lactic Acid)-Based Biomaterials: Synthesis, Modification and Applications
Xiao, L., Wang, B., Yang, G., & Gauthier, M. (2012) · InTech eBooks · doi:10.5772/23927 · Cited by 283 · see citing works
Quantitative metabolic imaging using endogenous fluorescence to detect stem cell differentiation
Quinn, K. P., Sridharan, G. V., Hayden, R. S., Kaplan, D. L., Lee, K., & Georgakoudi, I. (2013) · Scientific Reports · doi:10.1038/srep03432 · Cited by 278 · see citing works
Self-citationMierke, C. T. (2019) · Reports on Progress in Physics · doi:10.1088/1361-6633/ab1628 · Cited by 262 · see citing works
Clinical and preclinical translation of cell-based therapies using adipose tissue-derived cells
Gimble, J., Guilak, F., & Bunnell, B. A. (2010) · Stem Cell Research & Therapy · doi:10.1186/scrt19 · Cited by 261 · see citing works
Self-citationAdipose Tissue Engineering for Soft Tissue Regeneration
Choi, J. H. K., Gimble, J. M., Lee, K., Marra, K. G., Rubin, J. P., Yoo, J. J., Vunjak‐Novakovic, G., & Kaplan, D. L. (2010) · Tissue Engineering Part B Reviews · doi:10.1089/ten.teb.2009.0544 · Cited by 254 · see citing works
Self-citationMembrane Potential Controls Adipogenic and Osteogenic Differentiation of Mesenchymal Stem Cells
Sundelacruz, S., Levin, M., & Kaplan, D. L. (2008) · PLoS ONE · doi:10.1371/journal.pone.0003737 · Cited by 249 · see citing works
Self-citationBarud, H. G. O., Barud, H. D. S., Cavicchioli, M., do Amaral, T. S., de Oliveira, O. B., Santos, D. M., Petersen, A. L. D. O. A., Celes, F. S., Borges, V. M., de Oliveira, C. I., et al. (2015) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2015.04.007 · Cited by 245 · see citing works
Potential of Agarose/Silk Fibroin Blended Hydrogel for in Vitro Cartilage Tissue Engineering
Singh, Y. P., Bhardwaj, N., & Mandal, B. B. (2016) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.6b08285 · Cited by 240 · see citing works
Correia, C. O., Bhumiratana, S., Yan, L., Oliveira, A. L., Gimble, J. M., Rockwood, D. N., Kaplan, D. L., Sousa, R. A., Reis, R. L., & Vunjak‐Novakovic, G. (2012) · Acta Biomaterialia · doi:10.1016/j.actbio.2012.03.019 · Cited by 235 · see citing works
Self-citationRodríguez, M. J., Brown, J. E., Giordano, J. E. M., Lin, S. J., Omenetto, F. G., & Kaplan, D. L. (2016) · Biomaterials · doi:10.1016/j.biomaterials.2016.11.046 · Cited by 221 · see citing works
Self-citationWound healing effect of electrospun silk fibroin nanomatrix in burn-model
Ju, H. W., Lee, O. J., Lee, J. M., Moon, B. M., Park, H. J., Park, Y. R., Lee, M. C., Kim, S. H., Chao, J. R., Ki, C. S., et al. (2015) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2015.12.055 · Cited by 209 · see citing works
Concise Review: Adipose-Derived Stromal Cells for Skeletal Regenerative Medicine
Lévi, B., & Longaker, M. T. (2011) · Stem Cells · doi:10.1002/stem.612 · Cited by 198 · see citing works
Adipose-Derived Stem Cells in Tissue Regeneration: A Review
Zuk, P. A. (2013) · ISRN Stem Cells · doi:10.1155/2013/713959 · Cited by 185 · see citing works
Chang, K.-H., Liao, H.-T., & Chen, J. (2013) · Acta Biomaterialia · doi:10.1016/j.actbio.2013.06.046 · Cited by 166 · see citing works
Human Adipose Stem Cells: From Bench to Bedside
De Francesco, F., Ricci, G., D'Andrea, F., Nicoletti, G. F., & Ferraro, G. A. (2015) · Tissue Engineering Part B: Reviews · doi:10.1089/ten.teb.2014.0608 · Cited by 163 · see citing works
3D electrospun silk fibroin nanofibers for fabrication of artificial skin
Sheikh, F. A., Ju, H. W., Lee, J. M., Moon, B. M., Park, H. J., Lee, O. J., Kim, J.-H., Kim, D., & Park, C. H. (2014) · Nanomedicine Nanotechnology Biology and Medicine · doi:10.1016/j.nano.2014.11.007 · Cited by 161 · see citing works
Analysis of in vitro secretion profiles from adipose-derived cell populations
Blaber, S. P., Webster, R. A., Hill, C. J., Breen, E. J., Kuah, D., Vesey, G., & Herbert, B. R. (2012) · Journal of Translational Medicine · doi:10.1186/1479-5876-10-172 · Cited by 159 · see citing works
Combining stem cells and biomaterial scaffolds for constructing tissues and cell delivery
Sakiyama-Elbert (2008) · StemBook · doi:10.3824/stembook.1.1.1 · Cited by 153 · see citing works
Silk Fibroin as a Green Material
DeBari, M. K., King, C. I., Altgold, T. A., & Abbott, R. D. (2021) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.1c00493 · Cited by 150 · see citing works
Hronik‐Tupaj, M., Rice, W. L., Cronin‐Golomb, M., Kaplan, D. L., & Georgakoudi, I. (2011) · BioMedical Engineering OnLine · doi:10.1186/1475-925x-10-9 · Cited by 149 · see citing works
Self-citationIn vitro 3D Full‐Thickness Skin‐Equivalent Tissue Model Using Silk and Collagen Biomaterials
Bellas, E., Seiberg, M., Garlick, J. A., & Kaplan, D. L. (2012) · Macromolecular Bioscience · doi:10.1002/mabi.201200262 · Cited by 148 · see citing works
Self-citationJanani, G., Kumar, M., Chouhan, D., Moses, J. C., Gangrade, A., Bhattacharjee, S., & Mandal, B. B. (2019) · ACS Applied Bio Materials · doi:10.1021/acsabm.9b00576 · Cited by 147 · see citing works
Adipose Tissue Fibrosis: Mechanisms, Models, and Importance
DeBari, M. K., & Abbott, R. D. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21176030 · Cited by 141 · see citing works
In Vitro 3D Model for Human Vascularized Adipose Tissue
Kang, J. H., Gimble, J. M., & Kaplan, D. L. (2009) · Tissue Engineering Part A · doi:10.1089/ten.tea.2008.0469 · Cited by 144 · see citing works
Self-citationHe, H., Tao, G., Wang, Y., Cai, R., Guo, P., Chen, L., Zuo, H., Zhao, P., & Xia, Q. (2017) · Materials Science and Engineering C · doi:10.1016/j.msec.2017.06.015 · Cited by 142 · see citing works
Adipose-Derived Stem Cells: Isolation, Characterization, and Differentiation Potential
Huang, S.-J., Fu, R., Shyu, W., Liu, S., Jong, G., Chiu, Y.-W., Wu, H.-S., Tsou, Y.-A., Cheng, C.-W., & Lin, S. (2013) · Cell Transplantation · doi:10.3727/096368912x655127 · Cited by 133 · see citing works
Adipogenic Potential of Adipose Stem Cell Subpopulations
Li, H., Zimmerlin, L., Marra, K. G., Donnenberg, V. S., Donnenberg, A. D., & Rubin, J. P. (2011) · Plastic & Reconstructive Surgery · doi:10.1097/prs.0b013e318221db33 · Cited by 131 · see citing works
Bombyx mori Silk Fibers: An Outstanding Family of Materials
Pereira, R. F. P., Silva, M. M. P., & Bermudez, V. D. Z. (2014) · Macromolecular Materials and Engineering · doi:10.1002/mame.201400276 · Cited by 130 · see citing works
Zhao, W., Li, X., Liu, X., Zhang, N., & Wen, X. (2014) · Materials Science and Engineering C · doi:10.1016/j.msec.2014.03.048 · Cited by 127 · see citing works
Yu, G., Wu, X., Dietrich, M. A., Polk, P., Scott, L. K., Ptitsyn, A. A., & Gimble, J. M. (2010) · Cytotherapy · doi:10.3109/14653241003649528 · Cited by 126 · see citing works
Self-citationAcharya, C., Ghosh, S. K., & Kundu, S. C. (2008) · Journal of Materials Science Materials in Medicine · doi:10.1007/s10856-008-3408-3 · Cited by 126 · see citing works
Materials-Directed Differentiation of Mesenchymal Stem Cells for Tissue Engineering and Regeneration
Leach, J. K., & Whitehead, J. (2017) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.6b00741 · Cited by 126 · see citing works
An Injectable Adipose Matrix for Soft-Tissue Reconstruction
Wu, I., Nahas, Z., Kimmerling, K. A., Rosson, G. D., & Elisseeff, J. H. (2012) · Plastic & Reconstructive Surgery · doi:10.1097/prs.0b013e31824ec3dc · Cited by 121 · see citing works
Wang, X., Sun, L., Maffini, M. V., Soto, A., Sonnenschein, C., & Kaplan, D. L. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.01.118 · Cited by 120 · see citing works
Self-citationPark, S. Y., Ki, C. S., Park, Y. H., Jung, H. M., Woo, K. M., & Kim, H. J. (2010) · Tissue Engineering Part A · doi:10.1089/ten.tea.2009.0328 · Cited by 120 · see citing works
Microenvironmental Control of Adipocyte Fate and Function
Pope, B. D., Warren, C. R., Parker, K. K., & Cowan, C. A. (2016) · Trends in Cell Biology · doi:10.1016/j.tcb.2016.05.005 · Cited by 117 · see citing works
Philips, B. J., Grahovac, T. L., Valentin, J. E., Chung, C. W., Bliley, J. M., Pfeifer, M. E., Roy, S. B., Dreifuss, S. E., Kelmendi‐Doko, A., Kling, R. E., et al. (2013) · Plastic & Reconstructive Surgery · doi:10.1097/prs.0b013e31829fe5b1 · Cited by 115 · see citing works
Tissue Engineering Craniofacial Defects With Adult Stem Cells? Are We Ready Yet?
Zuk, P. A. (2008) · Pediatric Research · doi:10.1203/pdr.0b013e31816bdf36 · Cited by 112 · see citing works
Frydrych, M., Román, S., MacNeil, S., & Chen, B. (2015) · Acta Biomaterialia · doi:10.1016/j.actbio.2015.03.004 · Cited by 111 · see citing works
Combining Stem Cells and Biomaterial Scaffolds for Constructing Tissues and Cell Delivery
Willerth, S. M., & Sakiyama‐Elbert, S. E. (2019) · StemJournal · doi:10.3233/stj-180001 · Cited by 110 · see citing works
Liu, T.-L., Miao, J., Sheng, W., Xie, Y., Huang, Q., Shan, Y., & Yang, J. (2010) · Journal of Zhejiang University SCIENCE B · doi:10.1631/jzus.b0900163 · Cited by 107 · see citing works
WAT-on-a-chip: a physiologically relevant microfluidic system incorporating white adipose tissue
Loskill, P., Thiagarajan, S., Tharp, K. M., Lee-Montiel, F. T., Jeeawoody, S., Reese, W. M., Zushin, P. H., Stahl, A., & Healy, K. E. (2017) · Lab on a Chip · doi:10.1039/c6lc01590e · Cited by 106 · see citing works
Tissue-mimicking gelatin scaffolds by alginate sacrificial templates for adipose tissue engineering
Negrini, N. C., Bonnetier, M., Giatsidis, G., Orgill, D. P., Farè, S., & Marelli, B. (2019) · Acta Biomaterialia · doi:10.1016/j.actbio.2019.01.018 · Cited by 106 · see citing works
Control of stem cell fate by engineering their micro and nanoenvironment
Griffin, M. (2015) · World Journal of Stem Cells · doi:10.4252/wjsc.v7.i1.37 · Cited by 103 · see citing works
Wang, Z., Wang, J., Jin, Y., Luo, Z., Yang, W., Xie, H., Huang, K., & Wang, L. (2015) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.5b06804 · Cited by 103 · see citing works
Kuo, K.-C., Lin, R., Tien, H.-W., Wu, P.-Y., Li, Y., Melero‐Martin, J. M., & Chen, Y. (2015) · Acta Biomaterialia · doi:10.1016/j.actbio.2015.09.002 · Cited by 103 · see citing works
Silk as a Biomaterial to Support Long-Term Three-Dimensional Tissue Cultures
Abbott, R. D., Kimmerling, E. P., Cairns, D. M., & Kaplan, D. L. (2016) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.5b12114 · Cited by 101 · see citing works
Self-citationRanakoti, L., Gangil, B., Bhandari, P., Singh, T., Sharma, S. K., Singh, J., & Singh, S. (2023) · Molecules · doi:10.3390/molecules28020485 · Cited by 101 · see citing works
Mandal, B. B., & Kundu, S. C. (2008) · Biotechnology and Bioengineering · doi:10.1002/bit.21835 · Cited by 100 · see citing works
Xu, L., Zhang, Z., Jorgensen, A. M., Yang, Y., Jin, Q., Zhang, G., Cao, G., Fu, Y., Zhao, W., Ju, J., et al. (2023) · Materials Today Bio · doi:10.1016/j.mtbio.2023.100550 · Cited by 101 · see citing works
Cell-delivery therapeutics for adipose tissue regeneration☆
Bauer‐Kreisel, P., Göpferich, A. M., & Blunk, T. (2010) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2010.04.003 · Cited by 99 · see citing works
Stem cells from adipose tissue
Witkowska-Zimny, M., & Walenko, K. (2011) · Cellular & Molecular Biology Letters · doi:10.2478/s11658-011-0005-0 · Cited by 98 · see citing works
Adipose tissue engineering: state of the art, recent advances and innovative approaches
Tanzi, M. C., & Farè, S. (2009) · Expert Review of Medical Devices · doi:10.1586/erd.09.37 · Cited by 95 · see citing works
Krontiras, P., Gatenholm, P., & Hägg, D. A. (2014) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.33198 · Cited by 92 · see citing works
Biospinning by silkworms: Silk fiber matrices for tissue engineering applications
Mandal, B. B., & Kundu, S. C. (2009) · Acta Biomaterialia · doi:10.1016/j.actbio.2009.08.035 · Cited by 89 · see citing works
Choi, K. Y., Ajiteru, O., Hong, H., Suh, Y. J., Sultan, M. T., Lee, H., Lee, J. S., Lee, Y. J., Lee, O. J., Kim, S. H., et al. (2022) · Acta Biomaterialia · doi:10.1016/j.actbio.2023.04.034 · Cited by 86 · see citing works
Depolarization Alters Phenotype, Maintains Plasticity of Predifferentiated Mesenchymal Stem Cells
Sundelacruz, S., Levin, M. G., & Kaplan, D. L. (2013) · Tissue Engineering Part A · doi:10.1089/ten.tea.2012.0425.rev · Cited by 86 · see citing works
Self-citationMesenchymal Stem Cells for Craniofacial Tissue Regeneration: Designing Hydrogel Delivery Vehicles
Salinas, C. N., & Anseth, K. S. (2009) · Journal of Dental Research · doi:10.1177/0022034509341553 · Cited by 85 · see citing works
Natural Polymer‐Based Materials for Wound Healing Applications
Ho, T. T., Tran, H. A., Doan, V. K., Maitz, J., Li, Z., Wise, S. G., Lim, K. S., & Rnjak‐Kovacina, J. (2024) · Advanced NanoBiomed Research · doi:10.1002/anbr.202300131 · Cited by 88 · see citing works
Zhang, Q., Hubenak, J. R., Iyyanki, T. S., Alred, E. J., Turza, K. C., Davis, G., Chang, E. I.-F., Branch-Brooks, C. D., Beahm, E. K., & Butler, C. E. (2015) · Biomaterials · doi:10.1016/j.biomaterials.2015.09.024 · Cited by 84 · see citing works
Abbott, R. D., Wang, R. Y., Reagan, M. R., Chen, Y. G., Borowsky, F. E., Zieba, A., Marra, K. G., Rubin, J. P., Ghobrial, I. M., & Kaplan, D. L. (2016) · Advanced Healthcare Materials · doi:10.1002/adhm.201600211 · Cited by 81 · see citing works
Self-citationJongpaiboonkit, L., King, W. J., & Murphy, W. L. (2008) · Tissue Engineering Part A · doi:10.1089/ten.tea.2008.0096 · Cited by 80 · see citing works
Ji, S. T., Kim, H., Yun, J., Chung, J. S., & Kwon, S. (2017) · Stem Cells International · doi:10.1155/2017/3945403 · Cited by 77 · see citing works
Biomaterials for Three-Dimensional Cell Culture: From Applications in Oncology to Nanotechnology
Saydé, T., Hamoui, O. E., Aliès, B., Gaudin, K., Lespès, G., & Battu, S. (2021) · Nanomaterials · doi:10.3390/nano11020481 · Cited by 75 · see citing works
Fabrication of Porous Extracellular Matrix Scaffolds from Human Adipose Tissue
Choi, J. S., Yang, H.-J., Kim, B. S., Kim, J. D., Lee, S., Lee, E. K., Park, K., Cho, Y. W., & Lee, H. Y. (2009) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2009.0276 · Cited by 75 · see citing works
Silk Fibroin–Substrate Interactions at Heterogeneous Nanocomposite Interfaces
Grant, A. M., Kim, H. S., Dupnock, T. L., Hu, K., Yingling, Y. G., & Tsukruk, V. V. (2016) · Advanced Functional Materials · doi:10.1002/adfm.201601268 · Cited by 75 · see citing works
Liu, J., Yan, L., Yang, W. C., Lan, Y., Zhu, Q., Xu, H., Zheng, C., & Guo, R. (2019) · Bioactive Materials · doi:10.1016/j.bioactmat.2019.03.001 · Cited by 74 · see citing works
Vallée, M., Côté, J.-A., & Fradette, J. (2008) · Pathologie Biologie · doi:10.1016/j.patbio.2008.04.010 · Cited by 74 · see citing works
Hsu, V., Stransky, C. A., Bucky, L. P., & Percec, I. (2012) · Aesthetic Surgery Journal · doi:10.1177/1090820x12455658 · Cited by 74 · see citing works
Adipose Tissue Regeneration: A State of the Art
Casadei, A., Epis, R., Ferroni, L., Tocco, I., Gardin, C., Bressan, E. A., Sivolella, S., Vindigni, V., Pinton, P., Mucci, G., et al. (2012) · BioMed Research International · doi:10.1155/2012/462543 · Cited by 73 · see citing works
Song, M., Liu, Y., & Hui, L. (2017) · Molecular Medicine Reports · doi:10.3892/mmr.2017.7857 · Cited by 73 · see citing works
Sustainable Three-Dimensional Tissue Model of Human Adipose Tissue
Bellas, E., Marra, K. G., & Kaplan, D. L. (2013) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2012.0620 · Cited by 72 · see citing works
Self-citationSystematic tuning of pore morphologies and pore volumes in macroporous materials by freezing
Qian, L., Ahmed, A., Foster, A. J., Rannard, S. P., Cooper, A. I., & Zhang, H. (2009) · Journal of Materials Chemistry · doi:10.1039/b903461g · Cited by 72 · see citing works
Regulation of Stem Cell Fate by Nanomaterial Substrates
Mashinchian, O., Turner, L.-A., Dalby, M. J., Laurent, S., Shokrgozar, M. A., Bonakdar, S., Imani, M., & Mahmoudi, M. (2015) · Nanomedicine · doi:10.2217/nnm.14.225 · Cited by 71 · see citing works
Ferraro, G. A., Francesco, F. D., Nicoletti, G., Paino, F., Desiderio, V., Tirino, V., & D’Andrea, F. (2012) · Journal of Cellular Biochemistry · doi:10.1002/jcb.24443 · Cited by 71 · see citing works
Jiang, J., & Papoutsakis, E. T. (2012) · Advanced Healthcare Materials · doi:10.1002/adhm.201200169 · Cited by 71 · see citing works
Adipose stem cell-based soft tissue regeneration
Philips, B. J., Marra, K. G., & Rubin, J. P. (2011) · Expert Opinion on Biological Therapy · doi:10.1517/14712598.2012.644533 · Cited by 70 · see citing works
Silk Fibroin Scaffolds for Urologic Tissue Engineering
Sack, B. S., Mauney, J. R., & Estrada, C. R. (2016) · Current Urology Reports · doi:10.1007/s11934-015-0567-x · Cited by 69 · see citing works
Self-citationKayabölen, A., Keskin, D. S., Aykan, A., Karslıoğlu, Y., Zor, F., & Tezcaner, A. (2017) · Biomedical Materials · doi:10.1088/1748-605x/aa6a63 · Cited by 69 · see citing works
Liu, T., Xu, J., Pan, X., Ding, Z., Xie, H., Wang, X., & Xie, H. (2021) · Bioactive Materials · doi:10.1016/j.bioactmat.2021.01.015 · Cited by 69 · see citing works
Adipose tissue as a stem cell source for musculoskeletal regeneration
Gimble, J. M. (2010) · Frontiers in Bioscience-Scholar · doi:10.2741/s133 · Cited by 67 · see citing works
Self-citationHanson, A. D., Marvel, S. W., Bernacki, S. H., Banes, A. J., van Aalst, J. A., & Loboa, E. G. (2009) · Annals of Biomedical Engineering · doi:10.1007/s10439-009-9648-7 · Cited by 67 · see citing works
Injectable Silk Foams for Soft Tissue Regeneration
Bellas, E., Lo, T. J., Fournier, E., Brown, J. E., Abbott, R. D., Gil, E. S., Marra, K. G., Rubin, J. P., Leisk, G. G., & Kaplan, D. L. (2014) · Advanced Healthcare Materials · doi:10.1002/adhm.201400506 · Cited by 67 · see citing works
Self-citation
- Authors
- Mauney, J. R., Nguyen, T., Gillen, K., Kirker-Head, C., Gimble, J. M., & Kaplan, D. L.
- Affiliations
- Tufts University (US)
- Pennington Biomedical Research Center (US)
- Corresponding author
- Kaplan, David L.
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2007-12
- Volume
- 28
- Issue
- 35
- Pages
- 5280-5290
- ISSN
- 0142-9612 (ISSN-L)
- PMC
- PMC2695965
- OpenAlex
- W2095560028
- Cited by
- 373
- References
- 57
- Citations by year
- 2026: 4 · 2025: 8 · 2024: 9 · 2023: 10 · 2022: 12 · 2021: 22 · 2020: 11 · 2019: 21 · 2018: 16 · 2017: 29 · 2016: 25 · 2015: 25 · 2014: 35 · 2013: 34 · 2012: 30
- FWCI
- 9.15
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute of Biomedical Imaging and Bioengineering (R01EB003210, P41EB002520)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Silk-based biomaterials and applications
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- adipose tissue engineering, silk fibroin scaffolds, adipose-derived stem cells, bone marrow mesenchymal stem cells, adipogenesis, collagen scaffolds, polylactic acid scaffolds, scaffold degradation, cell seeding, macroporous scaffolds
- MeSH terms
- Adipose Tissue, Adult, Animals, Biocompatible Materials, Bone Marrow Cells, Cell Differentiation, Cells, Cultured, Fibroins, Humans, Male, Rats, Nude, Bombyx, Tissue Engineering, Silk, Rats, Tissue Scaffolds, Mesenchymal Stem Cells
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