The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Effects of Initial Seeding Density and Fluid Perfusion Rate on Formation of Tissue-Engineered Bone
Open accessAbstract
We describe a novel bioreactor system for tissue engineering of bone that enables cultivation of up to six tissue constructs simultaneously, with direct perfusion and imaging capability. The bioreactor was used to investigate the relative effects of initial seeding density and medium perfusion rate on the growth and osteogenic differentiation patterns of bone marrow–derived human mesenchymal stem cells (hMSCs) cultured on three-dimensional scaffolds. Fully decellularized bovine trabecular bone was used as a scaffold because it provided suitable “biomimetic” topography, biochemical composition, and mechanical properties for osteogenic differentiation of hMSCs. Trabecular bone plugs were completely denuded of cellular material using a serial treatment with hypotonic buffers and detergents, seeded with hMSCs, and cultured for 5 weeks. Increasing seeding density from 30 × 10 6 cells/mL to 60 × 10 6 cells/mL did not measurably influence the characteristics of tissue-engineered bone, in contrast to an increase in the perfusion rate from 100 μms −1 to 400 μms −1 , which radically improved final cell numbers, cell distributions throughout the constructs, and the amounts of bone proteins and minerals. Taken together, these findings suggest that the rate of medium perfusion during cultivation has a significant effect on the characteristics of engineered bone.
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Citations
Cites
Liu, X. S., Sajda, P., Saha, P. K., Wehrli, F. W., & Guo, X. E. (2006) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.060716 · Cited by 207
Cited by
Spiller, K. L., Nassiri, S., Witherel, C. E., Anfang, R. R., Ng, J., Nakazawa, K. R., Yu, T., & Vunjak-Novakovic, G. (2015) · Biomaterials · doi:10.1016/j.biomaterials.2014.10.017 · Cited by 773
Self-citationEngineered Microenvironments for Controlled Stem Cell Differentiation
Burdick, J. A., & Vunjak-Novakovic, G. (2009) · Tissue Engineering Part A · doi:10.1089/ten.tea.2008.0131 · Cited by 485
Self-citationEngineering anatomically shaped human bone grafts
Grayson, W. L., Fröhlich, M., Yeager, K., Bhumiratana, S., Chan, M. E., Cannizzaro, C., Wan, L. Q., Liu, X. S., Guo, X. E., & Vunjak-Novakovic, G. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0905439106 · Cited by 425
Self-citationCheng, C. W., Solorio, L. D., & Alsberg, E. (2014) · Biotechnology Advances · doi:10.1016/j.biotechadv.2013.12.012 · Cited by 358
Tissue Engineered Bone Grafts: Biological Requirements, Tissue Culture and Clinical Relevance
Frohlich, M., Grayson, W., Wan, L., Marolt, D., Drobnic, M., & Vunjak- Novakovic, G. (2008) · Current Stem Cell Research & Therapy · doi:10.2174/157488808786733962 · Cited by 342
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Metadata
- Authors
- Grayson, W. L., Bhumiratana, S., Cannizzaro, C., Chao, P.-H. G., Lennon, D. P., Caplan, A. I., & Vunjak-Novakovic, G.
- Venue
- Tissue Engineering Part A
- Publisher
- SAGE Publications
- Published
- 2008-07-11
- Volume
- 14
- Issue
- 11
- Pages
- 1809-1820
- ISSN
- 1937-3341 (ISSN-L)
- 1937-335X
- OpenAlex
- W2070921289
- Cited by
- 245
- References
- 1
- FWCI
- 10.23
- Type
- journal article
- Language
- en
- Open access
- green
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- Grayson, W. L., Bhumiratana, S., Cannizzaro, C., Chao, P.-H. G., Lennon, D. P., Caplan, A. I., & Vunjak-Novakovic, G. (2008). Effects of Initial Seeding Density and Fluid Perfusion Rate on Formation of Tissue-Engineered Bone. Tissue Engineering Part A. https://doi.org/10.1089/ten.tea.2007.0255
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