Engineered Microenvironments for Controlled Stem Cell Differentiation
Open accessAbstract
In a developing organism, tissues emerge from coordinated sequences of cell renewal, differentiation, and assembly that are orchestrated by spatial and temporal gradients of multiple regulatory factors. The composition, architecture, signaling, and biomechanics of the cellular microenvironment act in concert to provide the necessary cues regulating cell function in the developing and adult organism. With recent major advances in stem cell biology, tissue engineering is becoming increasingly oriented toward biologically inspired in vitro cellular microenvironments designed to guide stem cell growth, differentiation, and functional assembly. The premise is that to unlock the full potential of stem cells, at least some aspects of the dynamic three-dimensional (3D) environments that are associated with their renewal, differentiation, and assembly in native tissues need to be reconstructed. In the general context of tissue engineering, we discuss the environments for guiding stem cell function by an interactive use of biomaterial scaffolds and bioreactors, and focus on the interplay between molecular and physical regulatory factors. We highlight some illustrative examples of controllable cell environments developed through the interaction of stem cell biology and tissue engineering at multiple levels.
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Cells in the body perform bioactivities in response to stimulation from a highly complex, 3D microenvironment.
“Cells in our body perform bioactivities in response to the stimulation from a highly complex, 3D microenvironment (15,98,112).”
From Modeling physiological events in 2D vs. 3D cell culture (2017) · cites this paper as [15]
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Flagged first, then by citations
Research works printed but not linked: 1
- [58]Gerecht S Cannizzaro C Figallo E Elvassore N Vunjak-Novakovic G Masters J Palsson B Thomson J Bioreactors for the 3D cultivation of human embryonic stem cells Human Embryonic Stem Cells, Human Cell Culture Series Springer Verlag 2007
Cited by
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Cell-laden microengineered gelatin methacrylate hydrogels
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Modeling physiological events in 2D vs. 3D cell culture
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Synthetic alternatives to Matrigel
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Barrila, J., Radtke, A. L., Crabbé, A., Sarker, S. F., Herbst-Kralovetz, M. M., Ott, C. M., & Nickerson, C. A. (2010) · Nature Reviews Microbiology · doi:10.1038/nrmicro2423 · Cited by 302 · see citing works
Review: <i>in vitro</i> microvessel models
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3D Bioprinting of Tumor Models for Cancer Research
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Chondroitin sulfate glycosaminoglycan hydrogels create endogenous niches for neural stem cells
Karumbaiah, L., Enam, S. F., Brown, A. C., Saxena, T., Betancur, M. I., Barker, T. H., & Bellamkonda, R. V. (2015) · Bioconjugate Chemistry · doi:10.1021/acs.bioconjchem.5b00397 · Cited by 73 · see citing works
- Authors
- Burdick, J. A., & Vunjak-Novakovic, G.
- Affiliations
- University of Pennsylvania (US)
- Columbia University
- Corresponding author
- Burdick, Jason A.
- ORCID
- Burdick, Jason A.
- Venue
- Tissue Engineering Part A
- Publisher
- SAGE Publications
- Published
- 2008-08-08
- Volume
- 15
- Issue
- 2
- Pages
- 205-219
- ISSN
- 1937-3341 (ISSN-L)
- 1937-335X
- PMC
- PMC2716398
- OpenAlex
- W1977608305
- Cited by
- 485
- References
- 136
- Citations by year
- 2026: 4 · 2025: 7 · 2024: 7 · 2023: 23 · 2022: 18 · 2021: 15 · 2020: 21 · 2019: 24 · 2018: 22 · 2017: 35 · 2016: 33 · 2015: 36 · 2014: 36 · 2013: 45 · 2012: 44
- FWCI
- 2.84
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Tissue Engineering Part A (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Columbia Academic Commons (Columbia University)
- Funders
- National Institutes of Health (2r01hl076485-16)
- National Heart, Lung, and Blood Institute (R01-HL076485, HL076485)
- National Cancer Institute (CO 21631)
- National Institute of Biomedical Imaging and Bioengineering (EB-002520, P41EB002520)
- National Institute of Dental and Craniofacial Research (K22DE015761, DE16525, R01DE016525)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- stem cell differentiation, engineered microenvironments, biomaterial scaffolds, bioreactors, 3D cell culture, cell mechanics, tissue engineering, signal transduction, cell assemblies, histogenesis
- MeSH terms
- Animals, Biocompatible Materials, Cell Differentiation, Humans, Stem Cells, Bioreactors, Tissue Engineering, Biomimetics
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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
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