Cited by 4853D Printing in Biomedical ResearchCellular Mechanics and InteractionsTissue Engineering and Regenerative Medicine

Engineered Microenvironments for Controlled Stem Cell Differentiation

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Burdick, Jason A., Vunjak-Novakovic, Gordana·2009·Tissue Engineering Part A

Abstract

In a developing organism, tissues emerge from coordinated sequences of cell renewal, differentiation, and assembly that are orchestrated by spatial and temporal gradients of multiple regulatory factors. The composition, architecture, signaling, and biomechanics of the cellular microenvironment act in concert to provide the necessary cues regulating cell function in the developing and adult organism. With recent major advances in stem cell biology, tissue engineering is becoming increasingly oriented toward biologically inspired in vitro cellular microenvironments designed to guide stem cell growth, differentiation, and functional assembly. The premise is that to unlock the full potential of stem cells, at least some aspects of the dynamic three-dimensional (3D) environments that are associated with their renewal, differentiation, and assembly in native tissues need to be reconstructed. In the general context of tissue engineering, we discuss the environments for guiding stem cell function by an interactive use of biomaterial scaffolds and bioreactors, and focus on the interplay between molecular and physical regulatory factors. We highlight some illustrative examples of controllable cell environments developed through the interaction of stem cell biology and tissue engineering at multiple levels.

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Crossref · 10.1089/ten.tea.2008.0131

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  • Cells in the body perform bioactivities in response to stimulation from a highly complex, 3D microenvironment.

    “Cells in our body perform bioactivities in response to the stimulation from a highly complex, 3D microenvironment (15,98,112).”

    From Modeling physiological events in 2D vs. 3D cell culture (2017) · cites this paper as [15]

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Research works printed but not linked: 1

  1. [58]Gerecht S Cannizzaro C Figallo E Elvassore N Vunjak-Novakovic G Masters J Palsson B Thomson J Bioreactors for the 3D cultivation of human embryonic stem cells Human Embryonic Stem Cells, Human Cell Culture Series Springer Verlag 2007

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Authors
Burdick, J. A., & Vunjak-Novakovic, G.
Affiliations
  • University of Pennsylvania (US)
  • Columbia University
Corresponding author
Burdick, Jason A.
Venue
Tissue Engineering Part A
Published
2008-08-08
Volume
15
Issue
2
Pages
205-219
ISSN
  • 1937-3341 (ISSN-L)
  • 1937-335X
OpenAlex
W1977608305
Cited by
485
References
136
Citations by year
2026: 4 · 2025: 7 · 2024: 7 · 2023: 23 · 2022: 18 · 2021: 15 · 2020: 21 · 2019: 24 · 2018: 22 · 2017: 35 · 2016: 33 · 2015: 36 · 2014: 36 · 2013: 45 · 2012: 44
FWCI
2.84
Type
journal article
Language
en
Open access
Locations
  • Tissue Engineering Part A (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Columbia Academic Commons (Columbia University)
Funders
  • National Institutes of Health (2r01hl076485-16)
  • National Heart, Lung, and Blood Institute (R01-HL076485, HL076485)
  • National Cancer Institute (CO 21631)
  • National Institute of Biomedical Imaging and Bioengineering (EB-002520, P41EB002520)
  • National Institute of Dental and Craniofacial Research (K22DE015761, DE16525, R01DE016525)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
Keywords
stem cell differentiation, engineered microenvironments, biomaterial scaffolds, bioreactors, 3D cell culture, cell mechanics, tissue engineering, signal transduction, cell assemblies, histogenesis
MeSH terms
Animals, Biocompatible Materials, Cell Differentiation, Humans, Stem Cells, Bioreactors, Tissue Engineering, Biomimetics

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