The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Engineering anatomically shaped human bone grafts
Open accessAbstract
The ability to engineer anatomically correct pieces of viable and functional human bone would have tremendous potential for bone reconstructions after congenital defects, cancer resections, and trauma. We report that clinically sized, anatomically shaped, viable human bone grafts can be engineered by using human mesenchymal stem cells (hMSCs) and a “biomimetic” scaffold-bioreactor system. We selected the temporomandibular joint (TMJ) condylar bone as our tissue model, because of its clinical importance and the challenges associated with its complex shape. Anatomically shaped scaffolds were generated from fully decellularized trabecular bone by using digitized clinical images, seeded with hMSCs, and cultured with interstitial flow of culture medium. A bioreactor with a chamber in the exact shape of a human TMJ was designed for controllable perfusion throughout the engineered construct. By 5 weeks of cultivation, tissue growth was evidenced by the formation of confluent layers of lamellar bone (by scanning electron microscopy), markedly increased volume of mineralized matrix (by quantitative microcomputer tomography), and the formation of osteoids (histologically). Within bone grafts of this size and complexity cells were fully viable at a physiologic density, likely an important factor of graft function. Moreover, the density and architecture of bone matrix correlated with the intensity and pattern of the interstitial flow, as determined in experimental and modeling studies. This approach has potential to overcome a critical hurdle—in vitro cultivation of viable bone grafts of complex geometries—to provide patient-specific bone grafts for craniofacial and orthopedic reconstructions.
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Cites
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Craniofacial Tissue Engineering by Stem Cells
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Effects of Initial Seeding Density and Fluid Perfusion Rate on Formation of Tissue-Engineered Bone
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Self-citationAn image-based approach for designing and manufacturing craniofacial scaffolds
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Cited by
Bone Tissue Engineering: Recent Advances and Challenges
Amini, A. R., Laurencin, C. T., & Nukavarapu, S. P. (2012) · Critical Reviews in Biomedical Engineering · doi:10.1615/critrevbiomedeng.v40.i5.10 · Cited by 2,336
Nanotechnological strategies for engineering complex tissues
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Inflammation, fracture and bone repair
Loi, F., Córdova, L. A., Pajarinen, J., Lin, T.-H., Yao, Z., & Goodman, S. B. (2016) · Bone · doi:10.1016/j.bone.2016.02.020 · Cited by 1,293
Functional and Biomimetic Materials for Engineering of the Three-Dimensional Cell Microenvironment
Huang, G., Li, F., Zhao, X., Ma, Y., Li, Y., Lin, M., Jin, G., Lu, T. J., Genin, G. M., & Xu, F. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00094 · Cited by 825
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Self-citation
Metadata
- Authors
- Grayson, W. L., Fröhlich, M., Yeager, K., Bhumiratana, S., Chan, M. E., Cannizzaro, C., Wan, L. Q., Liu, X. S., Guo, X. E., & Vunjak-Novakovic, G.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2009-10-09
- Volume
- 107
- Issue
- 8
- Pages
- 3299-3304
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2094049785
- Cited by
- 425
- References
- 27
- FWCI
- 15.14
- Type
- journal article
- Language
- en
- Open access
- green
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- Grayson, W. L., Fröhlich, M., Yeager, K., Bhumiratana, S., Chan, M. E., Cannizzaro, C., Wan, L. Q., Liu, X. S., Guo, X. E., & Vunjak-Novakovic, G. (2010). Engineering anatomically shaped human bone grafts. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0905439106
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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