Kidney Spheroids Recapitulate Tubular Organoids Leading to Enhanced Tubulogenic Potency of Human Kidney-Derived Cells
Abstract
Cell-based approaches utilizing autologous human renal cells require their isolation, expansion in vitro, and reintroduction back into the host for renal tissue regeneration. Nevertheless, human kidney epithelial cells (hKEpCs) lose their phenotype, dedifferentiate, and assume the appearance of fibroblasts after relatively few passages in culture. We hypothesized that growth conditions may influence hKEpC phenotype and function. hKEpCs retrieved from human nephrectomy tissue samples showed the ability to reproducibly form kidney spheres when grown in suspension culture developed in nonadherent conditions. Genetic labeling and time-lapse microscopy indicated, at least in part, the aggregation of hKEpCs into 3D spheroids rather than formation of pure clonally expanded spheres. Characterization of hKEpC spheroids by real-time polymerase chain reaction and FACS analysis showed upregulation of some renal developmental and "stemness" markers compared with monolayer and mostly an EpCAM(+)CD24(+)CD133(+)CD44(+) spheroid cell phenotype. Oligonucleotide microarrays, which were used to identify global transcriptional changes accompanying spheroid formation, showed predominantly upregulation of cell matrix/cell contact molecules and cellular biogenesis processes and downregulation of cell cycle, growth, and locomotion. Accordingly, hKEpC spheroids slowly proliferated as indicated by low Ki-67 staining, but when grafted in low cell numbers onto the chorioallantoic membrane (CAM) of the chick embryo, they exclusively reconstituted various renal tubular epithelia. Moreover, efficient generation of kidney spheroids was observed after long-term monolayer culture resulting in reestablishment of tubulogenic capacity upon CAM grafting. Thus, generation of a tubular organoid in hKEpC spheroids may provide a functional benefit for kidney-derived cells in vivo.
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Spheroid cell culture has been developed for research on cancer, stem cells, and many organs, including liver, kidney, and heart.
“This approach has been developed in the research of cancer (7, 76, 102), stem cells (17, 120), and many organs (such as liver, kidney, heart, etc.) (3,16,64,75).”
From Modeling physiological events in 2D vs. 3D cell culture (2017) · cites this paper as [16]
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- Authors
- Buzhor, E., Harari-Steinberg, O., Omer, D., Metsuyanim, S., Jacob-Hirsch, J., Noiman, T., Dotan, Z., Goldstein, R. S., & Dekel, B.
- Affiliations
- Tel Aviv University (IL)
- Sheba Medical Center, Pediatric Stem Cell Research Institute, Edmond and Lili Safra Children's Hospital, Tel Hashomer, Israel.
- Bar-Ilan University (IL)
- Sheba Cancer Research Center, Sheba Medical Center, Tel Hashomer, Israel.
- Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.
- Sheba Medical Center (IL)
- Corresponding author
- Dekel, Benjamin
- Venue
- Tissue Engineering Part A
- Publisher
- SAGE Publications
- Published
- 2011-09
- Volume
- 17
- Issue
- 17-18
- Pages
- 2305-2319
- ISSN
- 1937-3341 (ISSN-L)
- 1937-335X
- OpenAlex
- W1969366572
- Cited by
- 72
- References
- 48
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 2 · 2023: 5 · 2022: 7 · 2021: 9 · 2020: 9 · 2019: 1 · 2018: 2 · 2017: 9 · 2016: 1 · 2015: 7 · 2014: 7 · 2013: 9 · 2012: 1
- FWCI
- 2.21
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Tissue Engineering Part A (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Renal and related cancers
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- kidney regeneration, 3D cell culture, spheroid formation, dedifferentiation, stem cell markers, gene expression changes
- MeSH terms
- Cells, Cultured, Flow Cytometry, Humans, Kidney, Organoids, Spheroids, Cellular, Cell Culture Techniques, Microarray Analysis
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