A Chitosan-Hyaluronan-Based Hydrogel-Hydrocolloid Supports In Vitro Culture and Differentiation of Human Mesenchymal Stem/Stromal Cells
Abstract
Three-dimensional (3D) cell culture platforms are increasingly utilized due to their ability to more closely mimic the in vivo microenvironment compared to traditional two-dimensional methods. Limitations of currently available 3D materials include lack of cell attachment, long polymerization times, and inclusion of undefined xenobiotics, and cytotoxic cross-linkers. Evaluated here is a unique hydrogel comprised of polyelectrolytic complex (PEC) fibers formed by hyaluronic acid and chitosan (CT). When hydrated with fetal bovine serum containing human mesenchymal stem/stromal cells (hMSCs), a hydrogel with an elastic modulus of 264±38 Pa formed in seconds with cells distributed throughout the matrix. Scanning electron microscopy showed a lattice-like meshwork of PEC fibers forming irregular compartments. hMSCs showed 48% viability during the first 24 h, with cell populations thereafter reaching a steady state for 14 days. hMSCs in the matrix were induced to differentiate to chondrogenic, osteogenic, and adipogenic phenotypes. Emergent features, at days 56 and 70, consisted of chondrogenesis on the surface of hydrogels induced to osteogenic and adipogenic phenotypes. Results indicate that this matrix may be useful for tissue engineering and disease modeling applications.
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Hyaluronic acid and mixtures of hyaluronic acid and chitosan have been extensively used in the growth and construction of neural organoids.
“Similarly, hyaluronic acid and mixtures of hyaluronic acid and chitosan have been extensively used in the growth and construction of neural organoids111–116.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [112]
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- Authors
- Lindborg, B. A., Brekke, J. H., Scott, C. M., Chai, Y. W., Ulrich, C., Sandquist, L., Kokkoli, E., & O'Brien, T. D.
- Affiliations
- University of Minnesota (US)
- Bioactive Regenerative Therapeutics, Inc., Duluth, Minnesota.
- Ascension (US)
- Stem Cell Institute, University of Minnesota, Minneapolis, Minnesota.
- Venue
- Tissue Engineering Part A
- Publisher
- SAGE Publications
- Published
- 2015-06
- Volume
- 21
- Issue
- 11-12
- Pages
- 1952-1962
- ISSN
- 1937-3341 (ISSN-L)
- 1937-335X
- OpenAlex
- W1937223179
- Cited by
- 37
- References
- 38
- Citations by year
- 2025: 2 · 2024: 2 · 2023: 1 · 2022: 3 · 2021: 6 · 2020: 2 · 2019: 6 · 2018: 6 · 2017: 3 · 2016: 5 · 2015: 1
- FWCI
- 2.06
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Tissue Engineering Part A (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- chitosan, hyaluronic acid, hydrogels, hydrocolloids, 3D cell culture, chondrogenic differentiation, osteogenic differentiation, adipogenesis, tissue engineering scaffolds, disease modeling
- MeSH terms
- Bone Marrow Cells, Cell Differentiation, Colloids, Humans, Hyaluronic Acid, Materials Testing, Microscopy, Electron, Scanning, Osteocytes, Phenotype, Rheology, Viscosity, Adipocytes, Cell Culture Techniques, Chondrocytes, Hydrogels, Tissue Engineering, Chitosan, Elastic Modulus, Mesenchymal Stem Cells, Cellular Microenvironment
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