The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
Laser Printing of Skin Cells and Human Stem Cells
Abstract
Laser printing based on laser-induced forward transfer (LIFT) is a new biofabrication technique for the arrangement of biological materials or living cells in well-defined patterns. In the current study, skin cell lines (fibroblasts/keratinocytes) and human mesenchymal stem cells (hMSC) were chosen for laser printing experiments due to their high potential in regeneration of human skin and new application possibilities of stem cell therapy. To evaluate the influence of LIFT on the cells, their survival rate, their proliferation and apoptotic activity, and the DNA damages and modifications of their cell surface markers were assessed and statistically evaluated over several days. The cells survived the transfer procedure with a rate of 98% +/- 1% standard error of the mean (skin cells) and 90% +/- 10% (hMSC), respectively. All used cell types maintain their ability to proliferate after LIFT. Further, skin cells and hMSC did not show an increase of apoptosis or DNA fragmentation. In addition, the hMSC keep their phenotype as proven by fluorescence activated cell sorting (FACS) analysis. This study demonstrates LIFT as a suitable technique for unharmed computer-controlled positioning of different cell types and a promising tool for future applications in the ex vivo generation of tissue replacements.
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Cites
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Cell and organ printing 1: Protein and cell printers
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“By combining cell solutions with materials that can form stable gels 14 it would further be possible to print 3D tissues layer-by-layer.”
Biological Laser Printing: A Novel Technique for Creating Heterogeneous 3-dimensional Cell Patterns
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Survival and Proliferative Ability of Various Living Cell Types after Laser-Induced Forward Transfer
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Cited by
3D bioprinting of tissues and organs
Murphy, S. V., & Atala, A. (2014) · Nature Biotechnology · doi:10.1038/nbt.2958 · Cited by 7,034
Recent advances in 3D printing of biomaterials
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Progress in 3D bioprinting technology for tissue/organ regenerative engineering
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The bioink: A comprehensive review on bioprintable materials
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Recent advances in biomaterials for 3D scaffolds: A review
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Metadata
- Authors
- Koch, L., Kuhn, S., Sorg, H., Gruene, M., Schlie, S., Gaebel, R., Polchow, B., Reimers, K., Stoelting, S., Ma, N., Vogt, P. M., Steinhoff, G., & Chichkov, B.
- Venue
- Tissue Engineering Part C: Methods
- Publisher
- SAGE Publications
- Published
- 2010-10
- Volume
- 16
- Issue
- 5
- Pages
- 847-854
- ISSN
- 1937-3384 (ISSN-L)
- 1937-3392
- OpenAlex
- W2014482422
- Cited by
- 476
- References
- 19
- FWCI
- 6.99
- Type
- journal article
- Language
- en
- Open access
- closed
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- Koch, L., Kuhn, S., Sorg, H., Gruene, M., Schlie, S., Gaebel, R., Polchow, B., Reimers, K., Stoelting, S., Ma, N., Vogt, P. M., Steinhoff, G., & Chichkov, B. (2010). Laser Printing of Skin Cells and Human Stem Cells. Tissue Engineering Part C: Methods. https://doi.org/10.1089/ten.tec.2009.0397
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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