The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Testosterone Signaling through Internalizable Surface Receptors in Androgen Receptor-free Macrophages
Open accessAbstract
Testosterone acts on cells through intracellular transcription-regulating androgen receptors (ARs). Here, we show that mouse IC-21 macrophages lack the classical AR yet exhibit specific nongenomic responses to testosterone. These manifest themselves as testosterone-induced rapid increase in intracellular free [Ca 2+], which is due to release of Ca 2+from intracellular Ca 2+stores. This Ca 2+mobilization is also inducible by plasma membrane-impermeable testosterone-BSA. It is not affected by the AR blockers cyproterone and flutamide, whereas it is completely inhibited by the phospholipase C inhibitor U-73122 and pertussis toxin. Binding sites for testosterone are detectable on the surface of intact IC-21 cells, which become selectively internalized independent on caveolae and clathrin-coated vesicles upon agonist stimulation. Internalization is dependent on temperature, ATP, cytoskeletal elements, phospholipase C, and G-proteins. Collectively, our data provide evidence for the existence of G-protein-coupled, agonist-sequestrable receptors for testosterone in plasma membranes, which initiate a transcription-independent signaling pathway of testosterone.
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Cites
Gene regulation by steroid hormones
Beato, M. (1989) · Cell · doi:10.1016/0092-8674(89)90237-7 · Cited by 3,383
Inhibition of oxytocin receptor function by direct binding of progesterone
Grazzini, E., Guillon, G., Mouillac, B., & Zingg, H. H. (1998) · Nature · doi:10.1038/33176 · Cited by 466
Identification of androgen receptors in normal human osteoblast-like cells.
Colvard, D. S., Eriksen, E. F., Keeting, P. E., Wilson, E. M., Lubahn, D. B., French, F. S., Riggs, B. L., & Spelsberg, T. C. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.3.854 · Cited by 462 · cited 1×
“Also, rat osteoblasts are known to be typical testosterone target cells containing intracellular AR (Colvard et al., 1989).”
Background
Cited by
Multiple Actions of Steroid Hormones—A Focus on Rapid, Nongenomic Effects
Falkenstein, E., Tillmann, H.-C., Christ, M., Feuring, M., & Wehling, M. (2000) · Pharmacological Reviews · doi:10.1016/s0031-6997(24)01468-6 · Cited by 991
The Roles of Androgen Receptors and Androgen-Binding Proteins in Nongenomic Androgen Actions
Heinlein, C. A., & Chang, C. (2002) · Molecular Endocrinology · doi:10.1210/me.2002-0070 · Cited by 568
Nongenomic steroid action: controversies, questions, and answers
LÖSEL, R. M., FALKENSTEIN, E., FEURING, M., SCHULTZ, A., TILLMANN, H.-C., ROSSOL-HASEROTH, K., & WEHLING, M. (2003) · Physiological Reviews · doi:10.1152/physrev.00003.2003 · Cited by 561
Non-genomic actions of androgens
Foradori, C. D., Weiser, M. J., & Handa, R. J. (2008) · Frontiers in Neuroendocrinology · doi:10.1016/j.yfrne.2007.10.005 · Cited by 493
The testis in immune privilege
Fijak, M., & Meinhardt, A. (2006) · Immunological Reviews · doi:10.1111/j.1600-065x.2006.00438.x · Cited by 483
Metadata
- Authors
- Benten, W. P. M., Lieberherr, M., Stamm, O., Wrehlke, C., Guo, Z., & Wunderlich, F.
- Venue
- Molecular Biology of the Cell
- Published
- 1999-10
- Volume
- 10
- Issue
- 10
- Pages
- 3113-3123
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- OpenAlex
- W2137889099
- Cited by
- 253
- References
- 3
- FWCI
- 5.44
- Type
- journal article
- Language
- en
- Open access
- green
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- Benten, W. P. M., Lieberherr, M., Stamm, O., Wrehlke, C., Guo, Z., & Wunderlich, F. (1999). Testosterone Signaling through Internalizable Surface Receptors in Androgen Receptor-free Macrophages. Molecular Biology of the Cell. https://doi.org/10.1091/mbc.10.10.3113
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