The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Activation of RhoA and ROCK Are Essential for Detachment of Migrating Leukocytes
Open accessAbstract
Detachment of the rear of the cell from its substratum is an important aspect of locomotion. The signaling routes involved in this adhesive release are largely unknown. One of the few candidate proteins to play a role is RhoA, because activation of RhoA in many cell types leads to contraction, a mechanism probably involved in detachment. To study the role of RhoA in detachment regulation, we analyzed several subsets of expert migratory leukocytes by video microscopy. In contrast to fast-migrating neutrophils, eosinophils do not detach the rear of the cell unless stimulated with serum. When measuring the amount of active RhoA, with the use of a GST-Rhotekin pulldown assay, we found that serum is an excellent activator of RhoA in granulocytes. Inhibition of RhoA or one of Rho's target proteins, the kinase ROCK, in neutrophils leads to the phenotype seen in eosinophils: the rear of the cell is firmly attached to the substratum, whereas the cell body is highly motile. ROCK-inhibition leads to impaired migration of granulocytes in filters, on glass, and through endothelial monolayers. Also, the ROCK signaling pathway is involved in changes of integrin-mediated adhesion. Eosinophil transduction by a tat-fusion construct containing active RhoA resulted in detachment stimulation in the presence of chemoattractant. From these results we conclude that activation of the RhoA-ROCK pathway is essential for detachment of migratory leukocytes.
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Cites
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Cited by
ROCKs: multifunctional kinases in cell behaviour
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Cell migration: Rho GTPases lead the way
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Rho‐kinase/ROCK: A key regulator of the cytoskeleton and cell polarity
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Tat peptide-mediated cellular delivery: back to basics
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Metadata
- Authors
- Alblas, J., Ulfman, L., Hordijk, P., & Koenderman, L.
- Venue
- Molecular Biology of the Cell
- Published
- 2001-07
- Volume
- 12
- Issue
- 7
- Pages
- 2137-2145
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- OpenAlex
- W2104485568
- Cited by
- 256
- References
- 28
- FWCI
- 6.62
- Type
- journal article
- Language
- en
- Open access
- green
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- Alblas, J., Ulfman, L., Hordijk, P., & Koenderman, L. (2001). Activation of RhoA and ROCK Are Essential for Detachment of Migrating Leukocytes. Molecular Biology of the Cell. https://doi.org/10.1091/mbc.12.7.2137
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