Mitotic spindle function in Saccharomyces cerevisiae requires a balance between different types of kinesin-related motors.
Open accessAbstract
Two Saccharomyces cerevisiae kinesin-related motors, Cin8p and Kip1p, perform an essential role in the separation of spindle poles during spindle assembly and a major role in spindle elongation. Cin8p and Kip1p are also required to prevent an inward spindle collapse prior to anaphase. A third kinesin-related motor, Kar3p, may act antagonistically to Cin8p and Kip1p since loss of Kar3p partially suppresses the spindle collapse in cin8 kip1 mutants. We have tested the relationship between Cin8p and Kar3p by overexpressing both motors using the inducible GAL1 promoter. Overexpression of KAR3 results in a shrinkage of spindle size and a temperature-dependent inhibition of the growth of wild-type cells. Excess Kar3p has a stronger inhibitory effect on the growth of cin8 kip1 mutants and can completely block anaphase spindle elongation in these cells. In contrast, overexpression of CIN8 leads to premature spindle elongation in all cells tested. This is the first direct demonstration of antagonistic motors acting on the intact spindle and suggests that spindle length is determined by the relative activity of Kar3p-like and Cin8p/Kip1p-like motors.
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Kar3 interaction with Cik1 alters motor structure and function
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Motor proteins of the eukaryotic cytoskeleton
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Self-citationGenome-wide Analysis Reveals Novel and Discrete Functions for Tubulin Carboxy-Terminal Tails
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Novelsfi1Alleles Uncover Additional Functions for Sfi1p in Bipolar Spindle Assembly and Function
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Motor guidance by long-range communication on the microtubule highway
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Complex Fluids and Soft Structures in the Human Body
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- Authors
- Saunders, W., Lengyel, V, & Hoyt, M. A.
- Affiliations
- University of Pittsburgh (US)
- Venue
- Molecular Biology of the Cell
- Published
- 1997-06
- Volume
- 8
- Issue
- 6
- Pages
- 1025-1033
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- PMC
- PMC305711
- OpenAlex
- W2126277736
- Cited by
- 160
- References
- 25
- Citations by year
- 2025: 6 · 2024: 2 · 2023: 2 · 2022: 2 · 2021: 6 · 2020: 6 · 2019: 9 · 2018: 4 · 2017: 5 · 2016: 3 · 2015: 6 · 2014: 6 · 2013: 6 · 2012: 4
- FWCI
- 3.09
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Molecular Biology of the Cell (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (2r01gm040714-10)
- National Institute of General Medical Sciences (GM-40714)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- mitotic spindle, spindle assembly, spindle elongation, anaphase, spindle length, Saccharomyces cerevisiae
- MeSH terms
- Anaphase, Cell Cycle, Cell Movement, Cell Survival, Fungal Proteins, Microtubule-Associated Proteins, Spindle Apparatus, Saccharomyces cerevisiae, Kinesins, Saccharomyces cerevisiae Proteins
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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
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