Cited by 160Microtubule and mitosis dynamicsFungal and yeast genetics researchGenomics and Chromatin Dynamics

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Mitotic spindle function in Saccharomyces cerevisiae requires a balance between different types of kinesin-related motors.

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Saunders, W, Lengyel, V, Hoyt, M A·1997·Molecular Biology of the Cell

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Abstract

Two Saccharomyces cerevisiae kinesin-related motors, Cin8p and Kip1p, perform an essential role in the separation of spindle poles during spindle assembly and a major role in spindle elongation. Cin8p and Kip1p are also required to prevent an inward spindle collapse prior to anaphase. A third kinesin-related motor, Kar3p, may act antagonistically to Cin8p and Kip1p since loss of Kar3p partially suppresses the spindle collapse in cin8 kip1 mutants. We have tested the relationship between Cin8p and Kar3p by overexpressing both motors using the inducible GAL1 promoter. Overexpression of KAR3 results in a shrinkage of spindle size and a temperature-dependent inhibition of the growth of wild-type cells. Excess Kar3p has a stronger inhibitory effect on the growth of cin8 kip1 mutants and can completely block anaphase spindle elongation in these cells. In contrast, overexpression of CIN8 leads to premature spindle elongation in all cells tested. This is the first direct demonstration of antagonistic motors acting on the intact spindle and suggests that spindle length is determined by the relative activity of Kar3p-like and Cin8p/Kip1p-like motors.

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Authors: Saunders, W., Lengyel, V, & Hoyt, M. A.
Affiliations: University of Pittsburgh (US)
Venue: Molecular Biology of the Cell
Publisher: American Society for Cell Biology (ASCB)
Published: 1997-06
Volume: 8
Issue: 6
Pages: 1025-1033
ISSN: 1059-1524 (ISSN-L)
1939-4586
DOI: 10.1091/mbc.8.6.1025
PMC: PMC305711
OpenAlex: W2126277736
Cited by: 160
References: 25
Citations by year: 2025: 6 · 2024: 2 · 2023: 2 · 2022: 2 · 2021: 6 · 2020: 6 · 2019: 9 · 2018: 4 · 2017: 5 · 2016: 3 · 2015: 6 · 2014: 6 · 2013: 6 · 2012: 4
FWCI: 3.09
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Locations: Molecular Biology of the Cell (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: National Institutes of Health (2r01gm040714-10)
National Institute of General Medical Sciences (GM-40714)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Keywords: mitotic spindle, spindle assembly, spindle elongation, anaphase, spindle length, Saccharomyces cerevisiae
MeSH terms: Anaphase, Cell Cycle, Cell Movement, Cell Survival, Fungal Proteins, Microtubule-Associated Proteins, Spindle Apparatus, Saccharomyces cerevisiae, Kinesins, Saccharomyces cerevisiae Proteins

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Mitotic spindle function in Saccharomyces cerevisiae requires a balance between different types of kinesin-related motors.

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