CoChat
Sign Up
Cited by 160Microtubule and mitosis dynamicsFungal and yeast genetics researchGenomics and Chromatin Dynamics

The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.

Mitotic spindle function in Saccharomyces cerevisiae requires a balance between different types of kinesin-related motors.

Open access
Saunders, W, Lengyel, V, Hoyt, M A·1997·Molecular Biology of the Cell
DOI 10.1091/mbc.8.6.1025PMC PMC305711
Open this paper in CoChat — free account

Abstract

Two Saccharomyces cerevisiae kinesin-related motors, Cin8p and Kip1p, perform an essential role in the separation of spindle poles during spindle assembly and a major role in spindle elongation. Cin8p and Kip1p are also required to prevent an inward spindle collapse prior to anaphase. A third kinesin-related motor, Kar3p, may act antagonistically to Cin8p and Kip1p since loss of Kar3p partially suppresses the spindle collapse in cin8 kip1 mutants. We have tested the relationship between Cin8p and Kar3p by overexpressing both motors using the inducible GAL1 promoter. Overexpression of KAR3 results in a shrinkage of spindle size and a temperature-dependent inhibition of the growth of wild-type cells. Excess Kar3p has a stronger inhibitory effect on the growth of cin8 kip1 mutants and can completely block anaphase spindle elongation in these cells. In contrast, overexpression of CIN8 leads to premature spindle elongation in all cells tested. This is the first direct demonstration of antagonistic motors acting on the intact spindle and suggests that spindle length is determined by the relative activity of Kar3p-like and Cin8p/Kip1p-like motors.

Overview

Integrity signals

Registry

Crossref · 10.1091/mbc.8.6.1025

Cites

25 of 25 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

The text we found for this paper looks like another document, so its claims and self-check weren’t run. The registry and citation checks still apply.

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The text we found for this paper looks like another document, so the self-check wasn’t run.

Citations

Cites

Flagged first, then by citations

Cited by

Sorted by citations

See all 160

Related

See all

Metadata

Authors
Saunders, W., Lengyel, V, & Hoyt, M. A.
Affiliations
University of Pittsburgh (US)
Venue
Molecular Biology of the Cell
Publisher
American Society for Cell Biology (ASCB)
Published
1997-06
Volume
8
Issue
6
Pages
1025-1033
ISSN
  • 1059-1524 (ISSN-L)
  • 1939-4586
DOI
10.1091/mbc.8.6.1025
PMC
PMC305711
OpenAlex
W2126277736
Cited by
160
References
25
Citations by year
2025: 6 · 2024: 2 · 2023: 2 · 2022: 2 · 2021: 6 · 2020: 6 · 2019: 9 · 2018: 4 · 2017: 5 · 2016: 3 · 2015: 6 · 2014: 6 · 2013: 6 · 2012: 4
FWCI
3.09
Type
journal article
Language
en
Open access
Locations
  • Molecular Biology of the Cell (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (2r01gm040714-10)
  • National Institute of General Medical Sciences (GM-40714)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Keywords
mitotic spindle, spindle assembly, spindle elongation, anaphase, spindle length, Saccharomyces cerevisiae
MeSH terms
Anaphase, Cell Cycle, Cell Movement, Cell Survival, Fungal Proteins, Microtubule-Associated Proteins, Spindle Apparatus, Saccharomyces cerevisiae, Kinesins, Saccharomyces cerevisiae Proteins

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review