The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
Drs2p-related P-type ATPases Dnf1p and Dnf2p Are Required for Phospholipid Translocation across the Yeast Plasma Membrane and Serve a Role in Endocytosis
Open accessAbstract
Plasma membranes in eukaryotic cells display asymmetric lipid distributions with aminophospholipids concentrated in the inner and sphingolipids in the outer leaflet. This asymmetry is maintained by ATP-driven lipid transporters whose identities are unknown. The yeast plasma membrane contains two P-type ATPases, Dnf1p and Dnf2p, with structural similarity to ATPase II, a candidate aminophospholipid translocase from bovine chromaffin granules. Loss of Dnf1p and Dnf2p virtually abolished ATP-dependent transport of NBD-labeled phosphatidylethanolamine, phosphatidylserine, and phosphatidylcholine from the outer to the inner plasma membrane leaflet, leaving transport of sphingolipid analogs unaffected. Labeling with trinitrobenzene sulfonic acid revealed that the amount of phosphatidylethanolamine exposed on the surface of Δdnf1Δdnf2 cells increased twofold relative to wild-type cells. Phosphatidylethanolamine exposure by Δdnf1Δdnf2 cells further increased upon removal of Drs2p, an ATPase II homolog in the yeast Golgi. These changes in lipid topology were accompanied by a cold-sensitive defect in the uptake of markers for bulk-phase and receptor-mediated endocytosis. Our findings demonstrate a requirement for Dnf1p and Dnf2p in lipid translocation across the yeast plasma membrane. Moreover, it appears that Dnf1p, Dnf2p and Drs2p each help regulate the transbilayer lipid arrangement in the plasma membrane, and that this regulation is critical for budding endocytic vesicles.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Bligh, E. G., & Dyer, W. J. (1959) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/o59-099 · Cited by 54,751
Rouser, G., Fleischer, S., & Yamamoto, A. (1970) · Lipids · doi:10.1007/bf02531316 · Cited by 3,274
A receptor for phosphatidylserine-specific clearance of apoptotic cells
Fadok, V. A., Bratton, D. L., Rose, D. M., Pearson, A., Ezekewitz, R. A. B., & Henson, P. M. (2000) · Nature · doi:10.1038/35011084 · Cited by 1,491
Biological Membranes as Bilayer Couples. A Molecular Mechanism of Drug-Erythrocyte Interactions
Sheetz, M. P., & Singer, S. J. (1974) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.71.11.4457 · Cited by 1,385
Robinson, J. S., Klionsky, D. J., Banta, L. M., & Emr, S. D. (1988) · Molecular and Cellular Biology · doi:10.1128/mcb.8.11.4936 · Cited by 967
Static and dynamic lipid asymmetry in cell membranes
Devaux, P. F. (1991) · Biochemistry · doi:10.1021/bi00219a001 · Cited by 827
Self-citationA gene encoding a P-type ATPase mutated in two forms of hereditary cholestasis
Bull, L. N., van Eijk, M. J. T., Pawlikowska, L., DeYoung, J. A., Juijn, J. A., Liao, M., Klomp, L. W. J., Lomri, N., Berger, R., Scharschmidt, B. R., et al. (1998) · Nature Genetics · doi:10.1038/ng0398-219 · Cited by 736
Seigneuret, M., & Devaux, P. F. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.12.3751 · Cited by 699
Self-citationHeterologousHIS3 Marker and GFP Reporter Modules for PCR-Targeting inSaccharomyces cerevisiae
Wach, A., Brachat, A., Alberti-Segui, C., Rebischung, C., & Philippsen, P. (1997) · Yeast · doi:10.1002/(sici)1097-0061(19970915)13:11<1065::aid-yea159>3.0.co;2-k · Cited by 696
The role of phospholipids and factor Va in the prothrombinase complex.
Rosing, J., Tans, G., Govers-Riemslag, J. W., Zwaal, R. F., & Hemker, H. C. (1980) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)86294-4 · Cited by 668
Cited by
Membrane lipids: where they are and how they behave
van Meer, G., Voelker, D. R., & Feigenson, G. W. (2008) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2330 · Cited by 7,157
Self-citationThe Distribution and Function of Phosphatidylserine in Cellular Membranes
Leventis, P. A., & Grinstein, S. (2010) · Annual Review of Biophysics · doi:10.1146/annurev.biophys.093008.131234 · Cited by 988
An Apoptotic ‘Eat Me’ Signal: Phosphatidylserine Exposure
Segawa, K., & Nagata, S. (2015) · Trends in Cell Biology · doi:10.1016/j.tcb.2015.08.003 · Cited by 783
Palmgren, M. G., & Nissen, P. (2011) · Annual Review of Biophysics · doi:10.1146/annurev.biophys.093008.131331 · Cited by 687
Exposure of phosphatidylserine on the cell surface
Nagata, S., Suzuki, J., Segawa, K., & Fujii, T. (2016) · Cell Death & Differentiation · doi:10.1038/cdd.2016.7 · Cited by 512
Metadata
- Authors
- Pomorski, T., Lombardi, R., Riezman, H., Devaux, P. F., van Meer, G., & Holthuis, J. C. M.
- Venue
- Molecular Biology of the Cell
- Published
- 2003-03
- Volume
- 14
- Issue
- 3
- Pages
- 1240-1254
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- OpenAlex
- W2135585405
- Cited by
- 353
- References
- 39
- FWCI
- 8.26
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Drs2p-related P-type ATPases Dnf1p and Dnf2p Are Required for Phospholipid Translocation across the Yeast Plasma Membrane and Serve a Role in Endocytosis. https://app.cochat.ai/papers/10.1091/mbc.e02-08-0501
- The paper
- Pomorski, T., Lombardi, R., Riezman, H., Devaux, P. F., van Meer, G., & Holthuis, J. C. M. (2003). Drs2p-related P-type ATPases Dnf1p and Dnf2p Are Required for Phospholipid Translocation across the Yeast Plasma Membrane and Serve a Role in Endocytosis. Molecular Biology of the Cell. https://doi.org/10.1091/mbc.e02-08-0501
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
