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Cited by 231Cellular Mechanics and InteractionsWound Healing and TreatmentsCell Adhesion Molecules Research

The DOI matches its Crossref record; 81 of the 81 references it cites were found in a registry or an index.

Myofibroblast Development Is Characterized by Specific Cell-Cell Adherens Junctions

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Hinz, B., Pittet, P., Smith-Clerc, J., Chaponnier, C., Meister, J.-J.·2004·Molecular Biology of the Cell
DOI 10.1091/mbc.e04-05-0386PMC PMC515361
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Abstract

Myofibroblasts of wound granulation tissue, in contrast to dermal fibroblasts, join stress fibers at sites of cadherin-type intercellular adherens junctions (AJs). However, the function of myofibroblast AJs, their molecular composition, and the mechanisms of their formation are largely unknown. We demonstrate that fibroblasts change cadherin expression from N-cadherin in early wounds to OB-cadherin in contractile wounds, populated with α-smooth muscle actin (α-SMA)-positive myofibroblasts. A similar shift occurs during myofibroblast differentiation in culture and seems to be responsible for the homotypic segregation of α-SMA-positive and -negative fibroblasts in suspension. AJs of plated myofibroblasts are reinforced by α-SMA–mediated contractile activity, resulting in high mechanical resistance as demonstrated by subjecting cell pairs to hydrodynamic forces in a flow chamber. A peptide that inhibits α-SMA–mediated contractile force causes the reorganization of large stripe-like AJs to belt-like contacts as shown for enhanced green fluorescent protein-α–catenin-transfected cells and is associated with a reduced mechanical resistance. Anti-OB-cadherin but not anti-N-cadherin peptides reduce the contraction of myofibroblast-populated collagen gels, suggesting that AJs are instrumental for myofibroblast contractile activity.

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Metadata

Authors
Hinz, B., Pittet, P., Smith-Clerc, J., Chaponnier, C., & Meister, J.-J.
Affiliations
  • École Polytechnique Fédérale de Lausanne (CH)
  • University of Geneva (CH)
ORCID
Venue
Molecular Biology of the Cell
Publisher
American Society for Cell Biology (ASCB)
Published
2004-09
Volume
15
Issue
9
Pages
4310-4320
ISSN
  • 1059-1524 (ISSN-L)
  • 1939-4586
DOI
10.1091/mbc.e04-05-0386
PMC
PMC515361
OpenAlex
W2096945892
Cited by
231
References
80
Citations by year
2026: 9 · 2025: 10 · 2024: 10 · 2023: 7 · 2022: 11 · 2021: 15 · 2020: 13 · 2019: 7 · 2018: 14 · 2017: 8 · 2016: 8 · 2015: 16 · 2014: 18 · 2013: 12 · 2012: 16
FWCI
2.04
Type
journal article
Language
en
Open access
Locations
  • Molecular Biology of the Cell (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion)
  • Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
  • Archive ouverte UNIGE (University of Geneva) (submittedVersion)
  • Archive ouverte UNIGE (University of Geneva) (submittedVersion, cc-by)
Funders
  • National Science Foundation
  • Biogen
  • Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (102150, 3100A0)
  • Université de Genève
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Health Sciences › Medicine › Surgery › Wound Healing and Treatments
  • Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
Keywords
myofibroblast differentiation, adherens junctions, N-cadherin, alpha-smooth muscle actin, cell contractility, alpha-catenin, stress fibers, mechanical resistance, collagen gel contraction, fibroblast differentiation
MeSH terms
Actins, Animals, Biomechanical Phenomena, Cell Differentiation, Cells, Cultured, Collagen, Female, Fibroblasts, Gels, Models, Biological, Skin, Wound Healing, Cadherins, Rats, Wistar, Adherens Junctions, Myoblasts, Rats

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