The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.
Binding of Src to Na+/K+-ATPase Forms a Functional Signaling Complex
Open accessAbstract
We have shown that ouabain activates Src, resulting in subsequent tyrosine phosphorylation of multiple effectors. Here, we tested if the Na+/K+-ATPase and Src can form a functional signaling complex. In LLC-PK1 cells the Na+/K+-ATPase and Src colocalized in the plasma membrane. Fluorescence resonance energy transfer analysis indicated that both proteins were in close proximity, suggesting a direct interaction. GST pulldown assay showed a direct, ouabain-regulated, and multifocal interaction between the α1 subunit of Na+/K+-ATPase and Src. Although the interaction between the Src kinase domain and the third cytosolic domain (CD3) of α1 is regulated by ouabain, the Src SH3SH2 domain binds to the second cytosolic domain constitutively. Functionally, binding of Src to either the Na+/K+-ATPase or GST-CD3 inhibited Src activity. Addition of ouabain, but not vanadate, to the purified Na+/K+-ATPase/Src complex freed the kinase domain and restored the Src activity. Consistently, exposure of intact cells to ouabain apparently increased the distance between the Na+/K+-ATPase and Src. Concomitantly, it also stimulated tyrosine phosphorylation of the proteins that are associated with the Na+/K+-ATPase. These new findings illustrate a novel molecular mechanism of signal transduction involving the interaction of a P-type ATPase and a nonreceptor tyrosine kinase.
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Cites
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Regulation, substrates and functions of src
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Structural changes in the calcium pump accompanying the dissociation of calcium
Toyoshima, C., & Nomura, H. (2002) · Nature · doi:10.1038/nature00944 · Cited by 894
Identification and characterization of a ouabain-like compound from human plasma.
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Structure and regulation of Src family kinases
Boggon, T. J., & Eck, M. J. (2004) · Oncogene · doi:10.1038/sj.onc.1208081 · Cited by 712
NMDA Channel Regulation by Channel-Associated Protein Tyrosine Kinase Src
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Src Tyrosine Kinase Is a Novel Direct Effector of G Proteins
Ma, Y.-C., Huang, J., Ali, S., Lowry, W., & Huang, X.-Y. (2000) · Cell · doi:10.1016/s0092-8674(00)00086-6 · Cited by 423
Self-citationSrc Family Tyrosine Kinases and Growth Factor Signaling
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Glutamate Transporter Coupling to Na,K-ATPase
Rose, E. M., Koo, J. C. P., Antflick, J. E., Ahmed, S. M., Angers, S., & Hampson, D. R. (2009) · Journal of Neuroscience · doi:10.1523/jneurosci.1081-09.2009 · Cited by 311
Identification of a Pool of Non-pumping Na/K-ATPase
Liang, M., Tian, J., Liu, L., Pierre, S., Liu, J., Shapiro, J., & Xie, Z.-J. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.m609181200 · Cited by 263
Self-citationCD36 and Na/K-ATPase-α1 Form a Proinflammatory Signaling Loop in Kidney
Kennedy, D. J., Chen, Y., Huang, W., Viterna, J., Liu, J., Westfall, K., Tian, J., Bartlett, D. J., Tang, W. H. W., Xie, Z., et al. (2013) · Hypertension · doi:10.1161/hypertensionaha.112.198770 · Cited by 101
Self-citationL’Hôte, V., Courbeyrette, R., Pinna, G., Cintrat, J., Le Pavec, G., Delaunay‐Moisan, A., Mann, C., & Thuret, J. (2021) · Aging Cell · doi:10.1111/acel.13447 · Cited by 37
Metadata
- Authors
- Tian, J., Cai, T., Yuan, Z., Wang, H., Liu, L., Haas, M., Maksimova, E., Huang, X.-Y., & Xie, Z.-J.
- Venue
- Molecular Biology of the Cell
- Published
- 2006-01
- Volume
- 17
- Issue
- 1
- Pages
- 317-326
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- OpenAlex
- W2101313549
- Cited by
- 358
- References
- 37
- FWCI
- 6.34
- Type
- journal article
- Language
- en
- Open access
- green
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