Cited by 63Nuclear Structure and FunctionGenomics and Chromatin DynamicsRNA and protein synthesis mechanisms

Pol I transcription and pre-rRNA processing are coordinated in a transcription-dependent manner in mammalian cells

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Kopp, K., Gasiorowski, J. Z., Chen, D., Gilmore, R., Norton, J. T., Wang, C., Leary, D. J., Chan, E.K.L. +2 more·2007·Molecular Biology of the Cell

Abstract

Pre-rRNA synthesis and processing are key steps in ribosome biogenesis. Although recent evidence in yeast suggests that these two processes are coupled, the nature of their association is unclear. In this report, we analyze the coordination between rDNA transcription and pre-rRNA processing in mammalian cells. We found that pol I transcription factor UBF interacts with pre-rRNA processing factors as analyzed by immunoprecipitations, and the association depends on active rRNA synthesis. In addition, injections of plasmids containing the human rDNA promoter and varying lengths of 18S rDNA into HeLa nuclei show that pol I transcription machinery can be recruited to rDNA promoters regardless of the product that is transcribed, whereas subgroups of pre-rRNA processing factors are recruited to plasmids only when specific pre-rRNA fragments are produced. Our observations suggest a model for sequential recruitment of pol I transcription factors and pre-rRNA processing factors to elongating pre-rRNA on an as-needed basis rather than corecruitment to sites of active transcription.

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Crossref · 10.1091/mbc.e06-03-0249

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Authors
Kopp, K., Gasiorowski, J. Z., Chen, D., Gilmore, R., Norton, J. T., Wang, C., Leary, D. J., Chan, E. K. L., Dean, D. A., & Huang, S.
Affiliations
  • Northwestern University (US)
  • *Departments of Cell and Molecular Biology and
  • University of Florida (US)
Venue
Molecular Biology of the Cell
Published
2007-02
Volume
18
Issue
2
Pages
394-403
ISSN
  • 1059-1524 (ISSN-L)
  • 1939-4586
OpenAlex
W2109313324
Cited by
63
References
56
Citations by year
2026: 3 · 2025: 3 · 2024: 7 · 2023: 5 · 2022: 2 · 2021: 3 · 2020: 1 · 2019: 5 · 2018: 3 · 2017: 1 · 2016: 3 · 2015: 2 · 2014: 3 · 2013: 9 · 2012: 2
FWCI
0.54
Type
journal article
Language
en
Open access
Locations
  • Molecular Biology of the Cell (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • American Heart Association
  • National Institutes of Health (5r33ca097761-03, HL‐71643)
  • National Heart, Lung, and Blood Institute (HL 71643, P01HL071643, R01HL059956, HL 59956)
  • National Cancer Institute (CA097761, R01CA077560, CA077560, R33 CA097761)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
Keywords
RNA polymerase I transcription, pre-rRNA processing, ribosome biogenesis, UBF, rDNA promoter, 18S rDNA, transcription factor recruitment, mammalian cells
MeSH terms
Dactinomycin, DNA, Ribosomal, HeLa Cells, Humans, Plasmids, Promoter Regions, Genetic, Ribonuclease, Pancreatic, RNA Precursors, RNA Processing, Post-Transcriptional, RNA, Ribosomal, Transcription, Genetic, RNA, Small Nucleolar, Pol1 Transcription Initiation Complex Proteins

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