The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Intraflagellar Transport and Functional Analysis of Genes Required for Flagellum Formation in Trypanosomes
Open accessAbstract
Intraflagellar transport (IFT) is the bidirectional movement of protein complexes required for cilia and flagella formation. We investigated IFT by analyzing nine conventional IFT genes and five novel putative IFT genes (PIFT) in Trypanosoma brucei that maintain its existing flagellum while assembling a new flagellum. Immunostaining against IFT172 or expression of tagged IFT20 or green fluorescent protein GFP::IFT52 revealed the presence of IFT proteins along the axoneme and at the basal body and probasal body regions of both old and new flagella. IFT particles were detected by electron microscopy and exhibited a strict localization to axonemal microtubules 3–4 and 7–8, suggesting the existence of specific IFT tracks. Rapid (>3 μm/s) bidirectional intraflagellar movement of GFP::IFT52 was observed in old and new flagella. RNA interference silencing demonstrated that all individual IFT and PIFT genes are essential for new flagellum construction but the old flagellum remained present. Inhibition of IFTB proteins completely blocked axoneme construction. Absence of IFTA proteins (IFT122 and IFT140) led to formation of short flagella filled with IFT172, indicative of defects in retrograde transport. Two PIFT proteins turned out to be required for retrograde transport and three for anterograde transport. Finally, flagellum membrane elongation continues despite the absence of axonemal microtubules in all IFT/PIFT mutant.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Nachury, M. V., Loktev, A. V., Zhang, Q., Westlake, C. J., Peränen, J., Merdes, A., Slusarski, D. C., Scheller, R. H., Bazan, J. F., Sheffield, V. C., et al. (2007) · Cell · doi:10.1016/j.cell.2007.03.053 · Cited by 1,384
A motility in the eukaryotic flagellum unrelated to flagellar beating.
Kozminski, K. G., Johnson, K. A., Forscher, P., & Rosenbaum, J. L. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.12.5519 · Cited by 1,007
Cole, D. G., Diener, D. R., Himelblau, A. L., Beech, P. L., Fuster, J. C., & Rosenbaum, J. L. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.141.4.993 · Cited by 898
Kozminski, K. G., Beech, P. L., & Rosenbaum, J. L. (1995) · The Journal of cell biology · doi:10.1083/jcb.131.6.1517 · Cited by 586
The DHC1b (DHC2) Isoform of Cytoplasmic Dynein Is Required for Flagellar Assembly
Pazour, G. J., Dickert, B. L., & Witman, G. B. (1999) · The Journal of Cell Biology · doi:10.1083/jcb.144.3.473 · Cited by 471
Cytoplasmic Dynein Heavy Chain 1b Is Required for Flagellar Assembly in Chlamydomonas
Porter, M. E., Bower, R., Knott, J. A., Byrd, P., & Dentler, W. (1999) · Molecular Biology of the Cell · doi:10.1091/mbc.10.3.693 · Cited by 333
Cited by
Ciliogenesis: building the cell's antenna
Ishikawa, H., & Marshall, W. F. (2011) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3085 · Cited by 961
Chapter Two Intraflagellar Transport (IFT)
Pedersen, L. B., & Rosenbaum, J. L. (2008) · Current Topics in Developmental Biology · doi:10.1016/s0070-2153(08)00802-8 · Cited by 521
Molecular Basis of Tubulin Transport Within the Cilium by IFT74 and IFT81
Bhogaraju, S., Cajanek, L., Fort, C., Blisnick, T., Weber, K., Taschner, M., Mizuno, N., Lamla, S., Bastin, P., Nigg, E. A., et al. (2013) · Science · doi:10.1126/science.1240985 · Cited by 317
Self-citationQin, J., Lin, Y., Norman, R. X., Ko, H. W., & Eggenschwiler, J. T. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1011410108 · Cited by 176
Novel Bilobe Components in Trypanosoma brucei Identified Using Proximity-Dependent Biotinylation
Morriswood, B., Havlicek, K., Demmel, L., Yavuz, S., Sealey-Cardona, M., Vidilaseris, K., Anrather, D., Kostan, J., Djinović-Carugo, K., Roux, K. J., et al. (2013) · Eukaryotic Cell · doi:10.1128/ec.00326-12 · Cited by 138
Metadata
- Authors
- Absalon, S., Blisnick, T., Kohl, L., Toutirais, G., Doré, G., Julkowska, D., Tavenet, A., & Bastin, P.
- Venue
- Molecular Biology of the Cell
- Published
- 2008-03
- Volume
- 19
- Issue
- 3
- Pages
- 929-944
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- OpenAlex
- W2167377309
- Cited by
- 186
- References
- 6
- FWCI
- 6.53
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Intraflagellar Transport and Functional Analysis of Genes Required for Flagellum Formation in Trypanosomes. https://app.cochat.ai/papers/10.1091/mbc.e07-08-0749
- The paper
- Absalon, S., Blisnick, T., Kohl, L., Toutirais, G., Doré, G., Julkowska, D., Tavenet, A., & Bastin, P. (2008). Intraflagellar Transport and Functional Analysis of Genes Required for Flagellum Formation in Trypanosomes. Molecular Biology of the Cell. https://doi.org/10.1091/mbc.e07-08-0749
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
