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Cited by 413Neuroscience and Neuropharmacology ResearchCellular Mechanics and InteractionsGenetics and Neurodevelopmental Disorders

The DOI matches its Crossref record; 51 of the 51 references it cites were found in a registry or an index.

Molecular Architecture of Synaptic Actin Cytoskeleton in Hippocampal Neurons Reveals a Mechanism of Dendritic Spine Morphogenesis

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Korobova, Farida, Svitkina, Tatyana·2010·Molecular Biology of the Cell
DOI 10.1091/mbc.e09-07-0596PMC PMC2801710
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Abstract

Excitatory synapses in the brain play key roles in learning and memory. The formation and functions of postsynaptic mushroom-shaped structures, dendritic spines, and possibly of presynaptic terminals, rely on actin cytoskeleton remodeling. However, the cytoskeletal architecture of synapses remains unknown hindering the understanding of synapse morphogenesis. Using platinum replica electron microscopy, we characterized the cytoskeletal organization and molecular composition of dendritic spines, their precursors, dendritic filopodia, and presynaptic boutons. A branched actin filament network containing Arp2/3 complex and capping protein was a dominant feature of spine heads and presynaptic boutons. Surprisingly, the spine necks and bases, as well as dendritic filopodia, also contained a network, rather than a bundle, of branched and linear actin filaments that was immunopositive for Arp2/3 complex, capping protein, and myosin II, but not fascin. Thus, a tight actin filament bundle is not necessary for structural support of elongated filopodia-like protrusions. Dynamically, dendritic filopodia emerged from densities in the dendritic shaft, which by electron microscopy contained branched actin network associated with dendritic microtubules. We propose that dendritic spine morphogenesis begins from an actin patch elongating into a dendritic filopodium, which tip subsequently expands via Arp2/3 complex-dependent nucleation and which length is modulated by myosin II-dependent contractility.

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Metadata

Authors
Korobova, F., & Svitkina, T.
Affiliations
University of Pennsylvania (US)
Corresponding author
Svitkina, Tatyana
ORCID
Venue
Molecular Biology of the Cell
Publisher
American Society for Cell Biology (ASCB)
Published
2010-01
Volume
21
Issue
1
Pages
165-176
ISSN
  • 1059-1524 (ISSN-L)
  • 1939-4586
DOI
10.1091/mbc.e09-07-0596
PMC
PMC2801710
OpenAlex
W2031516445
Cited by
399
References
51
Citations by year
2026: 7 · 2025: 13 · 2024: 19 · 2023: 22 · 2022: 21 · 2021: 25 · 2020: 28 · 2019: 23 · 2018: 30 · 2017: 24 · 2016: 30 · 2015: 27 · 2014: 36 · 2013: 34 · 2012: 27
FWCI
10.09
Type
journal article
Language
en
Open access
Locations
  • Molecular Biology of the Cell (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health
  • National Institute of General Medical Sciences (R01GM070898, GM 70898)
  • National Center for Research Resources (RR-22482, S10RR022482)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
Keywords
dendritic spine morphogenesis, hippocampal neurons, Arp2/3 complex, capping protein, myosin II, dendritic filopodia, presynaptic terminals, actin filament nucleation, microtubules, excitatory synapses
MeSH terms
Actins, Cytoskeletal Proteins, Cytoskeleton, Fluorescent Antibody Technique, Hippocampus, Models, Biological, Pseudopodia, Synapses, Biomarkers, Cell Shape, Dendritic Spines

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