The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
MiR-322/424 and -503 Are Induced during Muscle Differentiation and Promote Cell Cycle Quiescence and Differentiation by Down-Regulation of Cdc25A
Open accessAbstract
Induction of a G1 phase cell cycle arrest, caused primarily by the inhibition of cyclin-dependent-kinase 2 (cdk2), is a critical step in the differentiation of myoblasts into myotubes. Here, we report that two microRNAs, miR-322/424 and miR-503, are induced and promote cdk2 inhibition during myogenesis. These microRNAs down-regulate Cdc25A, the phosphatase responsible for removing inhibitory phosphorylation of cdk2, both in myoblasts differentiating into myotubes and in nonmuscle cells. Cdc25A is down-regulated during muscle differentiation by multiple pathways: action of these two microRNAs, proteasomal degradation of Cdc25A protein and transcriptional repression. Overexpression of Cdc25A or of cdk2 with mutations on T14 and Y15 (cdk2-AF), so that it cannot be inhibited by phosphorylation, decreases differentiation and differentiation-induced cell cycle quiescence. Introduction of miR-322/424 and miR-503 in heterologous cancer cells induces G1 arrest, which is also attenuated by overexpression of the cdk2-AF mutant. Until now Cdc25A and the inhibitory phosphorylation on T14 and Y15 of cdk2 have only been implicated in the intra-S phase checkpoint pathway after DNA damage. Our results reveal an unexpected role of Cdc25A down-regulation and the inhibitory phosphorylation of cdk2 T14 and Y15 in cell cycle quiescence during muscle differentiation and implicate two muscle differentiation-induced microRNAs in the process.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Ketting, R. F., Fischer, S. E. J., Bernstein, E., Sijen, T., Hannon, G. J., & Plasterk, R. H. A. (2001) · Genes & Development · doi:10.1101/gad.927801 · Cited by 1,852
Living with or without cyclins and cyclin-dependent kinases
Sherr, C. J., & Roberts, J. M. (2004) · Genes & Development · doi:10.1101/gad.1256504 · Cited by 1,089
Rapid Destruction of Human Cdc25A in Response to DNA Damage
Mailand, N., Falck, J., Lukas, C., Syljuåsen, R. G., Welcker, M., Bartek, J., & Lukas, J. (2000) · Science · doi:10.1126/science.288.5470.1425 · Cited by 787
Degradation of Cdc25A by β-TrCP during S phase and in response to DNA damage
Busino, L., Donzelli, M., Chiesa, M., Guardavaccaro, D., Ganoth, D., Valerio Dorrello, N., Hershko, A., Pagano, M., & Draetta, G. F. (2003) · Nature · doi:10.1038/nature02082 · Cited by 447
MicroRNAs regulate the expression of the alternative splicing factor nPTB during muscle development
Boutz, P. L., Chawla, G., Stoilov, P., & Black, D. L. (2007) · Genes & Development · doi:10.1101/gad.1500707 · Cited by 304
Kanemori, Y., Uto, K., & Sagata, N. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0501873102 · Cited by 128
RB, the conductor that orchestrates life, death and differentiation
Khidr, L., & Chen, P.-L. (2006) · Oncogene · doi:10.1038/sj.onc.1209612 · Cited by 123
Cited by
Kim, J., Kang, Y., Kojima, Y., Lighthouse, J. K., Hu, X., Aldred, M. A., McLean, D. L., Park, H., Comhair, S. A., Greif, D. M., et al. (2013) · Nature Medicine · doi:10.1038/nm.3040 · Cited by 356
Zhao, H., Wang, J., Gao, L., Wang, R., Liu, X., Gao, Z., Tao, Z., Xu, C., Song, J., Ji, X., et al. (2013) · Stroke · doi:10.1161/strokeaha.111.000504 · Cited by 188
Bovy, N., Blomme, B., Frères, P., Dederen, S., Nivelles, O., Lion, M., Carnet, O., Martial, J. A., Noël, A., Thiry, M., et al. (2015) · Oncotarget · doi:10.18632/oncotarget.3520 · Cited by 147
Nie, Y., Han, B.-M., Liu, X.-B., Yang, J.-J., Wang, F., Cong, X.-F., & Chen, X. (2011) · International Journal of Biological Sciences · doi:10.7150/ijbs.7.762 · Cited by 104
Xiao, F., zhang, W., Chen, L., Chen, F., Xie, H., Xing, C., Yu, X., Ding, S., Chen, K., Guo, H., et al. (2013) · Journal of Translational Medicine · doi:10.1186/1479-5876-11-195 · Cited by 71
Metadata
- Authors
- Sarkar, S., Dey, B. K., & Dutta, A.
- Venue
- Molecular Biology of the Cell
- Published
- 2010-07
- Volume
- 21
- Issue
- 13
- Pages
- 2138-2149
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- OpenAlex
- W2123631180
- Cited by
- 204
- References
- 7
- FWCI
- 7.56
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: MiR-322/424 and -503 Are Induced during Muscle Differentiation and Promote Cell Cycle Quiescence and Differentiation by Down-Regulation of Cdc25A. https://app.cochat.ai/papers/10.1091/mbc.e10-01-0062
- The paper
- Sarkar, S., Dey, B. K., & Dutta, A. (2010). MiR-322/424 and -503 Are Induced during Muscle Differentiation and Promote Cell Cycle Quiescence and Differentiation by Down-Regulation of Cdc25A. Molecular Biology of the Cell. https://doi.org/10.1091/mbc.e10-01-0062
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
