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Cited by 87Selenium in Biological SystemsOrganoselenium and organotellurium chemistryFungal and yeast genetics research

Jen1p: A High Affinity Selenite Transporter in Yeast

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McDermott, Joseph R., Rosen, Barry P., Liu, Zijuan·2010·Molecular Biology of the Cell
DOI 10.1091/mbc.e10-06-0513PMC PMC2982120
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Abstract

Selenium is a micronutrient in most eukaryotes, including humans, which is well known for having an extremely thin border between beneficial and toxic concentrations. Soluble tetravalent selenite is the predominant environmental form and also the form that is applied in the treatment of human diseases. To acquire this nutrient from low environmental concentrations as well as to avoid toxicity, a well-controlled transport system is required. Here we report that Jen1p, a proton-coupled monocarboxylate transporter in S. cerevisiae, catalyzes high-affinity uptake of selenite. Disruption of JEN1 resulted in selenite resistance, and overexpression resulted in selenite hypersensitivity. Transport assay showed that overexpression of Jen1p enables selenite accumulation in yeast compared with a JEN1 knock out strain, indicating the Jen1p transporter facilitates selenite accumulation inside cells. Selenite uptake by Jen1p had a K m of 0.91 mM, which is comparable to the K m for lactate. Jen1p transported selenite in a proton-dependent manner which resembles the transport mechanism for lactate. In addition, selenite and lactate can inhibit the transport of each other competitively. Therefore, we postulate selenite is a molecular mimic of monocarboxylates which allows selenite to be transported by Jen1p.

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Authors
McDermott, J. R., Rosen, B. P., & Liu, Z.
Affiliations
  • Oakland University (US)
  • Florida International University (US)
Corresponding author
Liu, Zijuan
ORCID
Venue
Molecular Biology of the Cell
Publisher
American Society for Cell Biology (ASCB)
Published
2010-11-15
Volume
21
Issue
22
Pages
3934-3941
ISSN
  • 1059-1524 (ISSN-L)
  • 1939-4586
DOI
10.1091/mbc.e10-06-0513
PMC
PMC2982120
OpenAlex
W2122713144
Cited by
87
References
2
Citations by year
2026: 3 · 2025: 6 · 2024: 7 · 2023: 5 · 2022: 2 · 2021: 8 · 2020: 4 · 2019: 4 · 2018: 5 · 2017: 9 · 2016: 9 · 2015: 8 · 2014: 3 · 2013: 6 · 2012: 1
FWCI
3.95
Type
journal article
Language
en
Open access
Licence
cc-by-nc-sa
Locations
  • Molecular Biology of the Cell (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
  • Florida International University Digital Commons (Florida International University) (submittedVersion, cc-by)
Funders
  • National Institutes of Health
  • National Institute of General Medical Sciences (GM55425, R01GM055425, R37GM055425)
  • National Institute of Environmental Health Sciences (ES016856, R15ES016856)
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Selenium in Biological Systems
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Toxicology › Organoselenium and organotellurium chemistry
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Keywords
selenium uptake, lactate transport, competitive inhibition, molecular mimicry, Saccharomyces cerevisiae
MeSH terms
Amino Acid Sequence, Biological Transport, Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone, Cell Membrane, Formates, Kinetics, Lactates, Mercury, Microscopy, Fluorescence, Molecular Sequence Data, Mutation, Nigericin, Saccharomyces cerevisiae, Sodium Selenite, Arsenites, Pyruvic Acid, Monocarboxylic Acid Transporters, Symporters, Saccharomyces cerevisiae Proteins

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