The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.
Mammalian Atg2 proteins are essential for autophagosome formation and important for regulation of size and distribution of lipid droplets
Open accessAbstract
Macroautophagy is an intracellular degradation system by which cytoplasmic materials are enclosed by the autophagosome and delivered to the lysosome. Autophagosome formation is considered to take place on the endoplasmic reticulum and involves functions of autophagy-related (Atg) proteins. Here, we report the identification and characterization of mammalian Atg2 homologues Atg2A and Atg2B. Simultaneous silencing of Atg2A and Atg2B causes a block in autophagic flux and accumulation of unclosed autophagic structures containing most Atg proteins. Atg2A localizes on the autophagic membrane, as well as on the surface of lipid droplets. The Atg2A region containing amino acids 1723–1829, which shows relatively high conservation among species, is required for localization to both the autophagic membrane and lipid droplet and is also essential for autophagy. Depletion of both Atg2A and Atg2B causes clustering of enlarged lipid droplets in an autophagy-independent manner. These data suggest that mammalian Atg2 proteins function both in autophagosome formation and regulation of lipid droplet morphology and dispersion.
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Cites
Autophagy regulates lipid metabolism
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Autophagy in immunity and inflammation
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Self-citationThe Role of Atg Proteins in Autophagosome Formation
Mizushima, N., Yoshimori, T., & Ohsumi, Y. (2011) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-092910-154005 · Cited by 2,978
Self-citationThe role of autophagy during the early neonatal starvation period
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Self-citationAutophagy in mammalian development and differentiation
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Self-citationNetwork organization of the human autophagy system
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Beclin 1 Forms Two Distinct Phosphatidylinositol 3-Kinase Complexes with Mammalian Atg14 and UVRAG
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Self-citationMizushima, N., Kuma, A., Kobayashi, Y., Yamamoto, A., Matsubae, M., Takao, T., Natsume, T., Ohsumi, Y., & Yoshimori, T. (2003) · Journal of Cell Science · doi:10.1242/jcs.00381 · Cited by 791
Self-citationAutophagy requires endoplasmic reticulum targeting of the PI3-kinase complex via Atg14L
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Self-citationLipid droplets: A dynamic organelle moves into focus
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Self-citationGuidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1
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Self-citationAutophagy in healthy aging and disease
Aman, Y., Schmauck-Medina, T., Hansen, M., Morimoto, R. I., Simon, A. K., Bjedov, I., Palikaras, K., Simonsen, A., Johansen, T., Tavernarakis, N., et al. (2021) · Nature Aging · doi:10.1038/s43587-021-00098-4 · Cited by 1,313
Autophagy and Neurodegeneration: Pathogenic Mechanisms and Therapeutic Opportunities
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Nguyen, T. N., Padman, B. S., Usher, J., Oorschot, V., Ramm, G., & Lazarou, M. (2016) · Journal of Cell Biology · doi:10.1083/jcb.201607039 · Cited by 668
Metadata
- Authors
- Velikkakath, A. K. G., Nishimura, T., Oita, E., Ishihara, N., & Mizushima, N.
- Venue
- Molecular Biology of the Cell
- Published
- 2012-03
- Volume
- 23
- Issue
- 5
- Pages
- 896-909
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- OpenAlex
- W2150723052
- Cited by
- 430
- References
- 11
- FWCI
- 15.94
- Type
- journal article
- Language
- en
- Open access
- green
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