Lymphocyte propionyl-CoA carboxylase and its activation by biotin are sensitive indicators of marginal biotin deficiency in humans
Open accessAbstract
Background: Marginal biotin deficiency may be a human teratogen. A biotin status indicator that is not dependent on renal function may be useful in studies of biotin status during pregnancy. A previous study of experimental biotin deficiency suggested that propionyl-coenzyme A carboxylase (PCC) activity in peripheral blood lymphocytes (PBLs) is a sensitive indicator of biotin status. Objective: We examined the utility of measuring PCC activity and the activation of PCC by biotin in detecting marginal biotin deficiency. Design: Marginal biotin deficiency was induced in 7 adults (3 women) by egg-white feeding for 28 d. Blood and urine were obtained on days 0, 14, and 28 (depletion phase) and 44 and 65 (repletion phase). PBLs were incubated with (activated) or without (control) biotin before PCC assay. The activation coefficient of PCC is the ratio of PCC activity in activated PBLs to that in control PBLs. The significance of differences for all measurements was tested by repeated-measures analysis of variance with Fisher’s post hoc test and Bonferroni correction. Results: Changes in the urinary excretion of biotin and of 3-hydroxyisovaleric acid confirmed that marginal biotin deficiency was successfully induced. By day 14, PCC activity had decreased (P < 0.0001) to below the lower limit of normal in all subjects. By day 28, the activation coefficient of PCC had increased significantly (P = 0.003) and was above the upper limit of normal in 6 of 7 subjects. Conclusion: PCC activity is the most sensitive indicator of biotin status tested to date. In future pregnancy studies, the use of lymphocyte PCC activity data should prove valuable in the assessment of biotin status.
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3-hydroxy-isovaleric acid has been demonstrated to have efficacy for inhibiting muscle wasting when used in conjunction with physical exercise.
“3-hydroxy-isovaleric acid (potentially from egg consumption) which has been demonstrated to have efficacy for inhibiting muscle wasting when used in conjunction of physical exercise.[40]”
From The microbiome of professional athletes differs from that of more sedentary subjects in composition and particularly at the functional metabolic level (2018) · cites this paper as [40]
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- Authors
- Stratton, S. L., Bogusiewicz, A., Mock, M. M., Mock, N. I., Wells, A. M., & Mock, D. M.
- Affiliations
- John L. McClellan Memorial Veterans Hospital (US)
- Corresponding author
- Mock, Donald M
- Venue
- The American Journal of Clinical Nutrition
- Publisher
- Elsevier BV
- Published
- 2006-08
- Volume
- 84
- Issue
- 2
- Pages
- 384-388
- ISSN
- 0002-9165 (ISSN-L)
- OpenAlex
- W4231992700
- Cited by
- 26
- References
- 23
- Citations by year
- 2023: 2 · 2020: 1 · 2017: 1 · 2015: 2 · 2014: 1 · 2013: 2 · 2012: 3
- FWCI
- 0.69
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- American Journal of Clinical Nutrition (publishedVersion, cc-by)
- Funders
- Vanderbilt University
- University of Arkansas for Medical Sciences
- Deutsches Krebsforschungszentrum
- National Institutes of Health
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Biotin and Related Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Metabolism and Genetic Disorders
- Health Sciences › Medicine › Rheumatology › Folate and B Vitamins Research
- Keywords
- propionyl-CoA carboxylase, peripheral blood lymphocytes, 3-hydroxyisovaleric acid, biotin supplementation, pregnancy, teratogens
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