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The Use of Antiavidin Antibody and Avidin-Biotin-Peroxidase Complex in Immunoperoxidase Technics
Abstract
Avidin has an extraordinary affinity for the small-molecule vitamin biotin. Covalently coupling biotin or avidin to peroxidase molecules does not interfere with their normal biochemical functions. The avidin or biotin molecules, either peroxidase conjugated or unconjugated, can be brought to the antigen sites by means of an antiavidin antibody. Several immunohistochemical staining technics based on this principal have been described. The method utilizing an avidin-biotin-peroxidase complex was found to be more sensitive than the unlabeled antibody (PAP) method. This method involved four sequential staining procedures: (1) primary antibody (goat anti-human antigen); (2) secondary antibody (rabbit antigoat IgG) added in relative excess; (3) goat antiavidin antibody; (4) avidin-biotin-peroxidase complex. The applications of this technic are discussed.
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Cited by
Fero, M. L., Rivkin, M., Tasch, M., Porter, P., Carow, C. E., Firpo, E., Polyak, K., Tsai, L.-H., Broudy, V., Perlmutter, R. M., et al. (1996) · Cell · doi:10.1016/s0092-8674(00)81239-8 · Cited by 1,457
Gunsilius, E., Duba, H.-C., Petzer, A. L., Kahler, C. M., Grunewald, K., Stockhammer, G., Gabl, C., Dirnhofer, S., Clausen, J., & Gastl, G. (2000) · The Lancet · doi:10.1016/s0140-6736(00)02241-8 · Cited by 337
Baker, C. S. R., Hall, R. J. C., Evans, T. J., Pomerance, A., Maclouf, J., Creminon, C., Yacoub, M. H., & Polak, J. M. (1999) · Arteriosclerosis, Thrombosis, and Vascular Biology · doi:10.1161/01.atv.19.3.646 · Cited by 296
A direct projection from superior colliculus to substantia nigra for detecting salient visual events
Comoli, E., Coizet, V., Boyes, J., Bolam, J. P., Canteras, N. S., Quirk, R. H., Overton, P. G., & Redgrave, P. (2003) · Nature Neuroscience · doi:10.1038/nn1113 · Cited by 295
Mild hypercholesterolemia is an early risk factor for the development of Alzheimer amyloid pathology
Pappolla, M. A., Bryant-Thomas, T. K., Herbert, D., Pacheco, J., Fabra Garcia, M., Manjon, M., Girones, X., Henry, T. L., Matsubara, E., Zambon, D., et al. (2003) · Neurology · doi:10.1212/01.wnl.0000070182.02537.84 · Cited by 247
Metadata
- Authors
- Hsu, S.-M., Raine, L., & Fanger, H.
- Venue
- American Journal of Clinical Pathology
- Publisher
- Oxford University Press (OUP)
- Published
- 1981-06-01
- Volume
- 75
- Issue
- 6
- Pages
- 816-821
- ISSN
- 0002-9173 (ISSN-L)
- 1943-7722
- OpenAlex
- W1550441131
- Cited by
- 1,052
- References
- 0
- FWCI
- 2.38
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: The Use of Antiavidin Antibody and Avidin-Biotin-Peroxidase Complex in Immunoperoxidase Technics. https://app.cochat.ai/papers/10.1093/ajcp/75.6.816
- The paper
- Hsu, S.-M., Raine, L., & Fanger, H. (1981). The Use of Antiavidin Antibody and Avidin-Biotin-Peroxidase Complex in Immunoperoxidase Technics. American Journal of Clinical Pathology. https://doi.org/10.1093/ajcp/75.6.816
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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