The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites
Open accessAbstract
Summary: CRISPRdirect is a simple and functional web server for selecting rational CRISPR/Cas targets from an input sequence. The CRISPR/Cas system is a promising technique for genome engineering which allows target-specific cleavage of genomic DNA guided by Cas9 nuclease in complex with a guide RNA (gRNA), that complementarily binds to a ∼20 nt targeted sequence. The target sequence requirements are twofold. First, the 5′-NGG protospacer adjacent motif (PAM) sequence must be located adjacent to the target sequence. Second, the target sequence should be specific within the entire genome in order to avoid off-target editing. CRISPRdirect enables users to easily select rational target sequences with minimized off-target sites by performing exhaustive searches against genomic sequences. The server currently incorporates the genomic sequences of human, mouse, rat, marmoset, pig, chicken, frog, zebrafish, Ciona, fruit fly, silkworm, Caenorhabditis elegans, Arabidopsis, rice, Sorghum and budding yeast. Availability: Freely available at http://crispr.dbcls.jp/. Contact: y-naito@dbcls.rois.ac.jp Supplementary information: Supplementary data are available at Bioinformatics online.
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“Conflict of interest: none declared.”
Cited by
Haeussler, M., Schönig, K., Eckert, H., Eschstruth, A., Mianné, J., Renaud, J.-B., Schneider-Maunoury, S., Shkumatava, A., Teboul, L., Kent, J., et al. (2016) · Genome biology · doi:10.1186/s13059-016-1012-2 · Cited by 1,756
Cysteinyl-tRNA synthetase governs cysteine polysulfidation and mitochondrial bioenergetics
Akaike, T., Ida, T., Wei, F.-Y., Nishida, M., Kumagai, Y., Alam, M. M., Ihara, H., Sawa, T., Matsunaga, T., Kasamatsu, S., et al. (2017) · Nature Communications · doi:10.1038/s41467-017-01311-y · Cited by 544
Glioblastoma Model Using Human Cerebral Organoids
Ogawa, J., Pao, G. M., Shokhirev, M. N., & Verma, I. M. (2018) · Cell Reports · doi:10.1016/j.celrep.2018.03.105 · Cited by 391
Genome-wide characterization of mammalian promoters with distal enhancer functions
Dao, L. T. M., Galindo-Albarrán, A. O., Castro-Mondragon, J. A., Andrieu-Soler, C., Medina-Rivera, A., Souaid, C., Charbonnier, G., Griffon, A., Vanhille, L., Stephen, T., et al. (2017) · Nature Genetics · doi:10.1038/ng.3884 · Cited by 281
Peng, S., Xiao, W., Ju, D., Sun, B., Hou, N., Liu, Q., Wang, Y., Zhao, H., Gao, C., Zhang, S., et al. (2019) · Science Translational Medicine · doi:10.1126/scitranslmed.aau7116 · Cited by 312
Metadata
- Authors
- Naito, Y., Hino, K., Bono, H., & Ui-Tei, K.
- Venue
- Bioinformatics
- Publisher
- Oxford University Press (OUP)
- Published
- 2014-12-09
- Volume
- 31
- Issue
- 7
- Pages
- 1120-1123
- ISSN
- 1367-4803 (ISSN-L)
- 1367-4811
- OpenAlex
- W2145782793
- Cited by
- 1,281
- References
- 12
- FWCI
- 17.16
- Type
- journal article
- Language
- en
- Open access
- hybrid
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