The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Error filtering, pair assembly and error correction for next-generation sequencing reads
Open accessAbstract
Motivation: Next-generation sequencing produces vast amounts of data with errors that are difficult to distinguish from true biological variation when coverage is low. Results: We demonstrate large reductions in error frequencies, especially for high-error-rate reads, by three independent means: (i) filtering reads according to their expected number of errors, (ii) assembling overlapping read pairs and (iii) for amplicon reads, by exploiting unique sequence abundances to perform error correction. We also show that most published paired read assemblers calculate incorrect posterior quality scores. Availability and implementation: These methods are implemented in the USEARCH package. Binaries are freely available at http://drive5.com/usearch. Contact: robert@drive5.com Supplementary information: Supplementary data are available at Bioinformatics online.
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Cited by
DADA2: High–resolution sample inference from Illumina amplicon data
Callahan, B. J., McMurdie, P. J., Rosen, M. J., Han, A. W., Johnson, A. J. A., & Holmes, S. P. (2016) · Nature Methods · doi:10.1038/nmeth.3869 · Cited by 35,698
VSEARCH: a versatile open source tool for metagenomics
(2016) · doi:10.7717/peerj.2584 · Cited by 10,773
Exact sequence variants should replace operational taxonomic units in marker-gene data analysis
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Salt-responsive gut commensal modulates TH17 axis and disease
Wilck, N., Matus, M. G., Kearney, S. M., Olesen, S. W., Forslund, K., Bartolomaeus, H., Haase, S., Mähler, A., Balogh, A., Markó, L., et al. (2017) · Nature · doi:10.1038/nature24628 · Cited by 1,310
Low‐gluten, nontransgenic wheat engineered with CRISPR/Cas9
Sánchez‐León, S., Gil‐Humanes, J., Ozuna, C. V., Giménez, M. J., Sousa, C., Voytas, D. F., & Barro, F. (2018) · Plant Biotechnology Journal · doi:10.1111/pbi.12837 · Cited by 587
Metadata
- Authors
- Edgar, R. C., & Flyvbjerg, H.
- Venue
- Bioinformatics
- Publisher
- Oxford University Press (OUP)
- Published
- 2015-07-02
- Volume
- 31
- Issue
- 21
- Pages
- 3476-3482
- ISSN
- 1367-4803 (ISSN-L)
- 1367-4811
- OpenAlex
- W2162667876
- Cited by
- 1,275
- References
- 8
- FWCI
- 28.05
- Type
- journal article
- Language
- en
- Open access
- bronze
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