The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Cellular background level of 8-oxo-7,8-dihydro-2'-deoxyguanosine: an isotope based method to evaluate artefactual oxidation of DNA during its extraction and subsequent work-up
Open accessAbstract
The measurement of oxidative damage to cellular DNA is a challenging analytical problem requiring highly sensitive and specific methods. In addition, artefactual DNA oxidation during its extraction and subsequent work-up may give rise to overestimated levels of oxidized DNA bases. In the present study, we have used (18)O-labelled 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) as an internal standard to evaluate the extent of artefactual DNA oxidation during the critical steps preceding the measurement. The labelled oxidized purine nucleoside was specifically generated in cellular DNA using the recently available generator of (18)O-labelled singlet oxygen. Artefactual DNA oxidation that could take place during the work-up increases the level of 8-oxodGuo but not of the (18)O-oxidized nucleoside. Therefore, the ratio between the two compounds, as measured by high performance liquid chromatography coupled to tandem mass spectrometry, allows an unambiguous comparison of different methodologies. The comparison of different DNA extraction protocols led to the conclusion that artefactual DNA oxidation during the extraction step could be minimized if: (i) nuclei are isolated after cell lysis; (ii) desferrioxamine, a transition metal chelator is added to the different extraction buffers; and (iii) sodium iodide (or alternatively guanidine thiocyanate) is used for DNA precipitation. It was also demonstrated that sodium iodide does not decompose the targeted oxidized purine nucleoside. In addition, three different DNA digestion protocols were evaluated and they were found to give rise to similar results. Using the best-studied protocol, the steady-state cellular background level of 8-oxodGuo, in a lymphocyte cell line, was determined to be approximately 0.5 lesions/10(6) DNA nucleosides.
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Cites
Vitamin C exhibits pro-oxidant properties
Podmore, I. D., Griffiths, H. R., Herbert, K. E., Mistry, N., Mistry, P., & Lunec, J. (1998) · Nature · doi:10.1038/33308 · Cited by 800
Self-citationFrelon, S., Douki, T., Ravanat, J.-L., Pouget, J.-P., Tornabene, C., & Cadet, J. (2000) · Chemical Research in Toxicology · doi:10.1021/tx000085h · Cited by 271
Self-citation8-Hydroxydeoxyguanosine and 8-hydroxyguanine as biomarkers of oxidative DNA damage
Helbock, H. J., Beckman, K. B., & Ames, B. N. (1999) · Methods in Enzymology · doi:10.1016/s0076-6879(99)00123-8 · Cited by 215
Singlet oxygen induces oxidation of cellular DNA.
Ravanat, J.-L., Di Mascio, P., Martinez, G. R., Medeiros, M. G., & Cadet, J. (2001) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)46360-6 · Cited by 42
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Cited by
Mouret, S., Baudouin, C., Charveron, M., Favier, A., Cadet, J., & Douki, T. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0604213103 · Cited by 684
Singlet Molecular Oxygen Reactions with Nucleic Acids, Lipids, and Proteins
Di Mascio, P., Martinez, G. R., Miyamoto, S., Ronsein, G. E., Medeiros, M. H. G., & Cadet, J. (2019) · Chemical Reviews · doi:10.1021/acs.chemrev.8b00554 · Cited by 659
Cadet, J., Douki, T., & Ravanat, J.-L. (2008) · Accounts of Chemical Research · doi:10.1021/ar700245e · Cited by 555
Self-citationThe genomics of oxidative DNA damage, repair, and resulting mutagenesis
Poetsch, A. R. (2020) · Computational and Structural Biotechnology Journal · doi:10.1016/j.csbj.2019.12.013 · Cited by 420
Chiorcea-Paquim, A.-M. (2022) · Molecules · doi:10.3390/molecules27051620 · Cited by 188
Metadata
- Authors
- Ravanat, J.-L.
- Venue
- Carcinogenesis
- Publisher
- Oxford University Press (OUP)
- Published
- 2002-11-01
- Volume
- 23
- Issue
- 11
- Pages
- 1911-1918
- ISSN
- 0143-3334 (ISSN-L)
- 1460-2180
- OpenAlex
- W2153781976
- Cited by
- 309
- References
- 4
- FWCI
- 4.90
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Ravanat, J.-L. (2002). Cellular background level of 8-oxo-7,8-dihydro-2'-deoxyguanosine: an isotope based method to evaluate artefactual oxidation of DNA during its extraction and subsequent work-up. Carcinogenesis. https://doi.org/10.1093/carcin/23.11.1911
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