Laminar and Areal Expression of Unc5d and Its Role in Cortical Cell Survival
Abstract
The UNC-5 family of netrin receptors is known to regulate axon guidance, cell migration, and cell survival. We have previously demonstrated that unc5d, one of the UNC-5 family member genes, is specifically expressed in layer 4 of the developing rat neocortex (Zhong Y, Takemoto M, Fukuda T, Hattori Y, Murakami F, Nakajima D, Nakayama M, Yamamoto N. 2004. Identification of the genes that are expressed in the upper layers of the neocortex. Cereb Cortex. 14:1144-1152). However, the role of UNC5D in cortical development is still unknown. In this study, we revealed that unc5d was highly expressed in the primary sensory areas of the mouse neocortex at around postnatal day 7. Netrin-4 was also found to be predominantly expressed in layer 4 of the sensory cortex and sensory thalamic nuclei. Cell surface binding assay showed that netrin-4 protein bound to UNC5D-expressing cells. An in vitro study further demonstrated that cell death of unc5d-expressing layer 4 cells was reduced by exogenous application of netrin-4 protein, whereas UNC5D is not sufficient to mediate the effect of netrin-4 in deep layer cells. Taken together, these results suggest that UNC5D is primarily expressed by layer 4 cells in the primary sensory areas of the developing neocortex and may mediate the effect of netrin-4 on cortical cell survival in a lamina-specific manner.
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We find one of the most deleterious regulatory SNCs in the Netrin receptor UNC5D, which is critical for axon guidance.
“we find one of the most deleterious regulatory SNCs in the Netrin receptor UNC5D, which is critical for axon guidance103.”
From A catalog of single nucleotide changes distinguishing modern humans from archaic hominins (2019) · cites this paper as [103]
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- Authors
- Takemoto, M., Hattori, Y., Zhao, H., Sato, H., Tamada, A., Sasaki, S., Nakajima, K., & Yamamoto, N.
- Affiliations
- The University of Osaka (JP)
- RIKEN Center for Brain Science (JP)
- Keio University (JP)
- Venue
- Cerebral Cortex
- Publisher
- Oxford University Press (OUP)
- Published
- 2011-01-07
- Volume
- 21
- Issue
- 8
- Pages
- 1925-1934
- ISSN
- 1047-3211 (ISSN-L)
- 1460-2199
- OpenAlex
- W2126513997
- Cited by
- 49
- References
- 58
- Citations by year
- 2026: 2 · 2025: 4 · 2023: 3 · 2022: 5 · 2021: 5 · 2020: 1 · 2019: 1 · 2018: 2 · 2017: 5 · 2016: 2 · 2014: 6 · 2013: 2 · 2012: 6
- FWCI
- 2.52
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cerebral Cortex (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science (22240041)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Keywords
- UNC5D, netrin-4, netrin receptors, neocortex development, layer 4, primary sensory cortex, sensory thalamus, neuronal cell death, axon guidance
- MeSH terms
- Netrins, Animals, Animals, Newborn, Cell Survival, Cells, Cultured, Female, Mice, Inbred C57BL, Mice, Inbred ICR, Nerve Growth Factors, Neural Pathways, Neurons, Pregnancy, Receptors, Cell Surface, Thalamus, Rats, Sprague-Dawley, Neocortex, Mice, Rats
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